Isolation and Characterization of Microsatellite Markers from the Endangered Karner Blue Butterfly Lycaeides Melissa Samuelis (Lepidoptera)

Anthony, Nicola M.; Gelembiuk, Gregory William; Raterman, Denise; Nice, Chris C.; ffrench-Constant, Richard H.

doi:10.1111/j.1601-5223.2001.00271.x

Cited by 17 publications

(11 citation statements)

References 15 publications

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“…Observations of excess homozygosity and null homozygote individuals at these novel loci further support this hypothesis. Interestingly, significant homozygote excess has also been noted in all other reports of butterfly microsatellites (P alo et al 1995; M eglécz and Solignac 1998; H arper et al 2000; A nthony et al 2001). Thus, high variability in the regions flanking microsatellite repeats may be widespread in butterflies and may contribute to the difficulty of microsatellite marker development in this group.…”

Section: Resultssupporting

confidence: 62%

“…Unfortunately, progress in this area has been hampered by the difficulty of isolating useful microsatellite markers from butterfly genomes (M eglécz and Solignac 1998; K eyghobadi et al 1999; N ève and Meglécz 2000). Very few microsatellite loci have been reported for butterflies or in fact for any Lepidoptera ( Palo et al 1995; B ogdanowicz et al 1997; M eglécz and Solignac 1998; K eyghobadi et al 1999; R eddy et al 1999; H arper et al 2000; A nthony et al 2001) and isolation and characterization of microsatellite markers is clearly more difficult in these insects than in most other organisms (M eglécz and Solignac 1998; N ève and Meglécz 2000). Here, we describe four novel microsatellite loci useful for population studies in the butterfly, Parnassius smintheus (Doubleday).…”

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confidence: 99%

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Isolation of novel microsatellite loci in the Rocky Mountain apollo butterfly, Parnassius smintheus

2002

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Section: Resultssupporting

confidence: 62%

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confidence: 99%

Isolation of novel microsatellite loci in the Rocky Mountain apollo butterfly, Parnassius smintheus

2002

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“…Subsequently, we scored these SNPs by visualizing PCR products on 2% agarose gels following digestion with Taq α 1 and Hin dIII for gapdh and inv respectively. The four microsatellite markers we amplified were Msat4, Msat6, MsatZ12 and Msat201, which were designed from a L. melissa genomic DNA library (Anthony et al. 2001) and were known to be polymorphic within and among L. melissa and L. idas populations (Anthony et al.…”

Section: Methodsmentioning

confidence: 99%

Secondary contact between Lycaeides idas and L. melissa in the Rocky Mountains: extensive admixture and a patchy hybrid zone

et al. 2010

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Studies of hybridization have increased our understanding of the nature of species boundaries, the process of speciation, and the effects of hybridization on the evolution of populations and species. In the present study we use genetic and morphological data to determine the outcome and consequences of secondary contact and hybridization between the butterfly species Lycaeides idas and L. melissa in the Rocky Mountains. Admixture proportions estimated from structure and geographical cline analysis indicate L. idas and L. melissa have hybridized extensively in the Rocky Mountains and that reproductive isolation was insufficient to prevent introgression for much of the genome. Geographical patterns of admixture suggest that hybridization between L. idas and L. melissa has led to the formation of a hybrid zone. The hybrid zone is relatively wide, given estimates of dispersal for Lycaeides butterflies, and does not show strong evidence of cline concordance among characters. We believe the structure of the Lycaeides hybrid zone might be best explained by the patchy distribution of Lycaeides, local extinction and colonization of habitat patches, environmental variation and weak overall selection against hybrids. We found no evidence that hybridization in the Rocky Mountains has resulted in the formation of independent hybrid species, in contrast to the outcome of hybridization between L. idas and L. melissa in the Sierra Nevada. Finally, our results suggest that differences in male morphology between L. idas and L. melissa might contribute to isolation, or perhaps even that selection has favoured the spread of L. melissa male genitalia alleles.

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“…PCR coamplification of fragments primed at [1 microsatellite locus often occur due to the presence of multilocus microsatellites (microsatellite families) that share nucleotide sequence similarity at genome regions that flank the tandem repeat (Zhang 2004;Meglécz et al 2004; Van't Hof et al 2007). Difficulties are encountered because alleles that originate from independently segregating loci typically are scored, and render genetic markers unsuitable for population or linkage analysis (Anthony et al 2001;Fauvelot et al 2006;Anderson et al 2007). Microsatellite families are known among insect species (Meglécz et al 2007), but appear pronounced within lepidopteran species due to a yet undescribed common ancestry that is shared among genomes.…”

Section: Introductionmentioning

confidence: 99%

A Helitron-Like Transposon Superfamily from Lepidoptera Disrupts (GAAA)n Microsatellites and is Responsible for Flanking Sequence Similarity within a Microsatellite Family

et al. 2010

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Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A + T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA)n microsatellite loci, and was named the microsatellite-associated interspersed nuclear element ( MINE-1). Non-autonomous MINE-1 superfamily members also were identified downstream of (GAAA)n microsatellites within Bombyx mori and Pectinophora gossypiella genomes. Of 316 (GAAA)n microsatellites from the B. mori whole genome sequence, 201 (63.6%) have associated autonomous or non-autonomous MINE-1 elements. Autonomous B. mori MINE-1s a encode a helicase and endonuclease domain RepHel-like protein (BMHELp1) indicating their classification as Helitron-like transposons and were renamed Helitron1_BM. Transposition of MINE-1 members in Lepidoptera has resulted in the disruption of (GAAA)n microsatellite loci, has impacted the application of microsatellite-based genetic markers, and suggests genome sequence that flanks TT/AA dinucleotides may be required for target site recognition by RepHel endonuclease domains. Abstract Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A ? T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA) n microsatellite loci, and was named the microsatellite-associated interspersed nuclear element (MINE-1). Non-autonomous MINE-1 superfamily members also were identified downstream of (GAAA) n microsatellites within Bombyx mori and Pectinophora gossypiella genomes. Of 316 (GAAA) n microsatellites from the B. mori whole genome sequence, 201 (63.6%) have associated autonomous or non-autonomous MINE-1 elements. Autonomous B. mori MINE-1s a encode a helicase and endonuclease domain RepHel-like protein (BMHELp1) indicating their classification as Helitron-like transposons and were renamed Helitron1_BM. Transposition of MINE-1 members in Lepidoptera has resulted in the disruption of (GAAA) n microsatellite loci, has impacted the application of microsatellite-based genetic markers, and suggests genome sequence that flanks TT/AA dinucleotides may be required for target site recognition by RepHel endonuclease domains.

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Isolation and Characterization of Microsatellite Markers from the Endangered Karner Blue Butterfly Lycaeides Melissa Samuelis (Lepidoptera)

Cited by 17 publications

References 15 publications

Isolation of novel microsatellite loci in the Rocky Mountain apollo butterfly, Parnassius smintheus

Isolation of novel microsatellite loci in the Rocky Mountain apollo butterfly, Parnassius smintheus

Secondary contact between Lycaeides idas and L. melissa in the Rocky Mountains: extensive admixture and a patchy hybrid zone

A Helitron-Like Transposon Superfamily from Lepidoptera Disrupts (GAAA)n Microsatellites and is Responsible for Flanking Sequence Similarity within a Microsatellite Family

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