2018
DOI: 10.1007/s12041-018-1012-y
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Isolation and characterization of microsatellite marker loci in the Wagner’s mustached bat Pteronotus psilotis (Chiroptera: Mormoopidae) and cross-amplification in other related species

Abstract: Pteronotus psilotis, a mormoopid bat, is an insectivorous, gregarious and strict cave-dwelling species that is found areas between the sea level and an elevation of about 1000 masl. This species is present in diverse habitats ranging from rain forest to dry deciduous forest. Nine microsatellite loci were developed for Wagner's mustached bat, Pteronotus psilotis using the next-generation sequencing approach, and their utility for population genetics studies was assessed. All loci were polymorphic (7-15 alleles)… Show more

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“…Thirteen polymorphic microsatellite markers were selected for the two species (P. fulvus and P. gymnonotus); 12 of these markers had been previously designed and characterized for Wagner's mustached bat (Pteronotus psilotis): Pps1, Pps3, Pps6, Pps7 [62], Pps10-Pps17 (this study, Table S3) and one was originally designed for Jamaican fruit bat (Artibeus jamaicensis): NAC8 [63]. Allele identification was carried out with the ABI PRISM 370xl equipment with LIZ (GeneScan™ 500 ® LIZ Size Standard) and the alleles' size was determined with the software GeneMarker v. 2.4.2 (SoftGenetics, LLC, State College, PA, USA).…”
Section: Dna Extraction Sequencing Editing and Alignmentmentioning
confidence: 99%
“…Thirteen polymorphic microsatellite markers were selected for the two species (P. fulvus and P. gymnonotus); 12 of these markers had been previously designed and characterized for Wagner's mustached bat (Pteronotus psilotis): Pps1, Pps3, Pps6, Pps7 [62], Pps10-Pps17 (this study, Table S3) and one was originally designed for Jamaican fruit bat (Artibeus jamaicensis): NAC8 [63]. Allele identification was carried out with the ABI PRISM 370xl equipment with LIZ (GeneScan™ 500 ® LIZ Size Standard) and the alleles' size was determined with the software GeneMarker v. 2.4.2 (SoftGenetics, LLC, State College, PA, USA).…”
Section: Dna Extraction Sequencing Editing and Alignmentmentioning
confidence: 99%