Isolation and characterization of microsatellite loci in <i>Alasmidonta heterodon</i> (Bivalvia: Unionidae)

Shaw, Kristine; King, Timothy L.; Lellis, William A.; Eackles, Michael S.

doi:10.1111/j.1471-8286.2005.01235.x

Cited by 6 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microsatellite markers for unionids are now being developed at a fast pace, and include five published sets (Eackles & King, 2002; Geist et al ., 2003; Jones et al ., 2004; Shaw et al ., 2006; Zanatta & Murphy, 2006). Using microsatellite markers, significant population structure was found in the European pearl mussel, Margaritifera margaritifera.…”

Section: Introductionmentioning

confidence: 99%

The phylogeographical and management implications of genetic population structure in the imperiled snuffbox mussel, Epioblasma triquetra (Bivalvia: Unionidae)

Zanatta

Murphy

2008

Biological Journal of the Linnean Society

View full text Add to dashboard Cite

Seven populations of the imperiled snuffbox mussel, Epioblasma triquetra, were sampled from across the central basin of North America. Samples were genotyped using 15 microsatellite DNA loci, and maternal history was inferred using mitochondrial DNA (mtDNA) cytochrome c oxidase subunit-I (COI) sequences. Populations in the Clinch and St Francis rivers were quite distinct in their mtDNA. The population in the St Francis River had a unique, fixed haplotype. Among a suite of haplotypes, the population in the Clinch River had two unique haplotypes of common ancestry. The other populations were dominated by a common haplotype, which also occurred in the Clinch River population. Analysis of DNA microsatellites revealed much greater divergences and showed significant genetic structure between populations in the formerly glaciated regions. Divergence has occurred between the populations, as evidenced by moderate to high fixation indices (FST and RST values) and nearly perfect assignment tests. These results indicate the occurrence of three glacial refugia for E. triquetra: the Tennessee River, rivers south of the Ozark Crest, and the lower Ohio River drainage near the confluence with the Mississippi. Populations in the lower Ohio River were likely to be responsible for the postglacial reinvasion into formerly glaciated regions, and into the upper Tennessee River drainage. The population of the St Francis River may constitute a distinct taxonomic entity. Conservation efforts, if necessary for this imperiled species, should not mix populations.

show abstract

Section: Introductionmentioning

confidence: 99%

The phylogeographical and management implications of genetic population structure in the imperiled snuffbox mussel, Epioblasma triquetra (Bivalvia: Unionidae)

Zanatta

Murphy

2008

Biological Journal of the Linnean Society

View full text Add to dashboard Cite

show abstract

“…This paucity of information is attributable, at least in part, to a lack of suitable molecular markers for studies on population-level genetic variation. Microsatellite markers have been developed to explore genetic structure in a number of organisms, including unionids (Eackles and King 2002;Shaw et al 2006;Zanatta and Murphy 2006). No microsatellite markers specific to the genus Elliptio have been reported to date, prompting our efforts to identify highly polymorphic microsatellite loci in the eastern elliptio.…”

mentioning

confidence: 99%

Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio

Ward

Shaw

Small

et al. 2010

Conservation Genet Resour

Self Cite

View full text Add to dashboard Cite

Fourteen microsatellite loci were isolated and characterized in the eastern elliptio (Elliptio complanata). The loci were screened across 24 individuals from a single population sampled in the Delaware River. All loci were found to be polymorphic with the number of alleles per locus ranging from 8 to 20. Observed heterozygosity ranged from 0.350 to 0.900. Deviations from Hardy-Weinberg equilibrium were observed in five of 14 microsatellite loci. In each case, failure to meet Hardy-Weinberg expectation was due to heterozygote deficiency. Cross-species amplification was tested using four additional Elliptio species: E. hoptenensis, E. shepardiana, E. dariensis, andE. icterina.

show abstract

Range-wide population genetic analysis of the endangered northern riffleshell mussel, Epioblasma torulosa rangiana (Bivalvia: Unionoida)

Zanatta

Murphy

2007

Conserv Genet

View full text Add to dashboard Cite

The northern riffleshell (Epioblasma torulosa rangiana) is a critically endangered unionoid species in need of conservation throughout its range. It is the only unionoid to be federally protected in both Canada and the U.S. We use sequences from two mtDNA genes and 15 microsatellite loci to assess genetic variation among 86 individuals from the four populations in the three remaining drainages in which E. t. rangiana is known to be reproducing. All of these populations are in formerly glaciated landscapes that emerged <10 kya. The mtDNA sequence data do not indicate significant geographic structure among the populations. However, allelic data from the microsatellite loci reveal highly significant population structuring. Individuals of E. t. rangiana can be assigned to their own river of origin with 98.8% accuracy. Significant isolation-by-distance occurs. This analysis will be useful to conservation managers in documenting the genetic structure, patterns of isolation, and genetic variability within and among populations of E. t. rangiana.

show abstract

Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)

Cited by 6 publications

References 5 publications

The phylogeographical and management implications of genetic population structure in the imperiled snuffbox mussel, Epioblasma triquetra (Bivalvia: Unionidae)

The phylogeographical and management implications of genetic population structure in the imperiled snuffbox mussel, Epioblasma triquetra (Bivalvia: Unionidae)

Development and characterization of microsatellite loci in Elliptio complanata (Mollusca: Unionidae) and cross-species amplification within the genus Elliptio

Range-wide population genetic analysis of the endangered northern riffleshell mussel, Epioblasma torulosa rangiana (Bivalvia: Unionoida)

Contact Info

Product

Resources

About