Isolation and Characterization of Mouse Testis Specific Serine/Threonine Kinase 5 Possessing Four Alternatively Spliced Variants

Wei, Youheng; Fu, Guolong; Hu, Hairong; Lin, Gang; Yang, Jingchun; Guo, Jinhu; Zhu, Qiquan; Liu, Yu

doi:10.5483/bmbrep.2007.40.5.749

Cited by 12 publications

(15 citation statements)

References 18 publications

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“…The VIb region forms the catalytic loop in the phosphotransfer process and is important for normal kinase activity. In vitro kinase activity assays demonstrated that mouse TSSK4-2 and 3 showed no kinase activity, which suggests that these two variants are pseudokinases and the VIb region is crucial for kinase activity (Wei et al, 2007). Referring to results of mouse TSSK4, we can infer that porcine TSSK4-3 is also a pseudokinase and has lost its protein phosphorylation activity, but further experimental analysis is necessary to clarify this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…: SM00220; Figure 3). The S_TKc domain functions by helping to form the precise spatial arrangement during the phosphotransfer process, and human and mouse TSSK proteins also have this domain (Wei et al, 2007). Multiple alignment for whole amino acids was performed between the porcine TSSK proteins and their orthologs in three other species (human, cattle, and mouse; Figure 4).…”

Section: Conserved Domains and Evolutionary Relationships Of Tssk Promentioning

confidence: 99%

“…The S_ TKc domain is helpful in forming the precise spatial arrangement required to catalyze the transfer of γ-phosphate from ATP to the hydroxyl group of serine, threonine, or tyrosine of the target protein (Kueng et al, 1997;Chen et al, 2005;Wei et al, 2007). To date, five members of the TSSK family, including TSSK1, TSSK2, TSSK3, TSSK4, and TSSK6 have been identified in mouse and human (Kueng et al, 1997;Zuercher et al, 2000;Visconti et al, 2001;Hao et al, 2004;Bucko-Justyna et al, 2005;Chen et al, 2005;Spiridonov et al, 2005;Gnanaraj et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Considering the integral role of protein phosphorylation, it is not surprising that it is involved in spermatogenesis. As a protein kinase family, the testis-specific serine kinases (TSSKs) are noteworthy because they are testis specific (Bielke et al, 1994;Kueng et al, 1997;Zuercher et al, 2000;Hao et al, 2004;Chen et al, 2005;Wei et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Cloning, sequence characterization, and expression patterns of members of the porcine TSSK family

Wang¹,

Huo²,

Wang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Testis-specific serine kinases (TSSKs) are a family of serine/ threonine kinases highly expressed in the testes that are responsible for regulating many spermatogenesis-related protein activities. Mutations in this family have a positive relationship with oligospermia and azoospermia in human and mouse. Here, five members of the TSSK family from a Banna mini-pig inbred line (BMI) were cloned, sequenced, and characterized. The (2015) full-length coding sequences of BMI TSSKs varied from 807 (TSSK3) to 1095 bp (TSSK1) and encoded 268 to 364 amino acids with molecular weights in the range 30.11 to 41.34 kDa. Following comparison with TSSK4 genes in other species, BMI TSSK4 was found to contain three alternatively spliced variants, inform1, inform 3, and inform 4. BMI TSSK1 and TSSK2 are co-localized on the Sus scrofa chromosome (SSC) 14, and consist of a single exon; TSSK3, TSSK4, and TSSK6 are on SSC6, SSC7, and SSC2, and consist of two, four, and one exon, respectively. Multiple protein sequence alignment and phylogenetic analysis showed that the regions spanning the S_TKc domains were more conserved between pig and other animals: with TSSK1 and TSSK2 and TSSK3 and TSSK6 displaying the greatest degree of homology across species, and the TSSK4 protein clearly distinct from other members. Multi-tissue RT-PCR showed BMI TSSK1, TSSK3, and TSSK4 were only expressed in the testes and seminal vesicle, TSSK2 was confined to testes only, while TSSK6 was expressed widely in adult tissues but was highest in the testes.

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Section: Discussionmentioning

confidence: 99%

Section: Conserved Domains and Evolutionary Relationships Of Tssk Promentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cloning, sequence characterization, and expression patterns of members of the porcine TSSK family

Wang¹,

Huo²,

Wang³

et al. 2015

Genet. Mol. Res.

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“…Step 10 spermatids are the earliest cells to show abnormalities and SSTK may be required for proper removal of histones from chromatin (Spiridonov et al, 2005 (Wei et al, 2007), and TESK1 [ID: T3-545], with the latter being under the control of CREM transcription activity (Toshima et al, 1998 .…”

Section: Microscopy Research and Techniquementioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

Hermo¹,

Pelletier²,

Cyr³

et al. 2009

Microscopy Res & Technique

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Spermiogenesis constitutes the steps involved in the metamorphosis of spermatids into spermatozoa. It involves modification of several organelles in addition to the formation of several structures including the flagellum and cytoplasmic droplet. The flagellum is composed of a neck region and middle, principal, and end pieces. The axoneme composed of nine outer microtubular doublets circularly arranged to form a cylinder around a central pair of microtubules is present throughout the flagellum. The middle and principal pieces each contain specific components such as the mitochondrial sheath and fibrous sheath, respectively, while outer dense fibers are common to both. A plethora of proteins are constituents of each of these structures, with each playing key roles in functions related to the fertility of spermatozoa. At the end of spermiogenesis, a portion of spermatid cytoplasm remains associated with the released spermatozoa, referred to as the cytoplasmic droplet. The latter has as its main feature Golgi saccules, which appear to modify the plasma membrane of spermatozoa as they move down the epididymal duct and hence may be partly involved in male gamete maturation. The end product of spermatogenesis is highly streamlined and motile spermatozoa having a condensed nucleus equipped with an acrosome. Spermatozoa move through the female reproductive tract and eventually penetrate the zona pellucida and bind to the egg plasma membrane. Many proteins have been implicated in the process of fertilization as well as a plethora of proteins involved in the development of spermatids and sperm, and these are high lighted in this review.

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Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197

Wei

Wang

et al. 2012

Mol Biol Rep

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The testis specific serine/threonine protein kinase family (Tssk) members play important roles in spermatogenesis and/or spermiogenesis. Similar to other Tssk family members, Tssk4 protein shows exclusive expression in testis, but its biochemical and biological functions are still largely unknown. In present work, we generate a polyclonal antibody which specifically recognizes Tssk4 but not the other three Tssk family members (Tssk1, Tssk2 and Tssk3). By using the qualified antibody, we show that Tssk4 protein is constantly expressed in testis from haploid round spermatids to morphological mature spermatozoa. Further experiments reveal that Tssk4 has autophosphorylation activity and self-association character in vitro. Importantly, we find that autophosphorylation of Tssk4 at Thr-197 in the T-loop region is essential to its kinase activity. Taken together, these findings suggest that autophosphorylation at Thr-197 plays a critical role in maintaining Tssk4 kinase activity, which might be involved in spermiogenesis.

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Isolation and Characterization of Mouse Testis Specific Serine/Threonine Kinase 5 Possessing Four Alternatively Spliced Variants

Cited by 12 publications

References 18 publications

Cloning, sequence characterization, and expression patterns of members of the porcine TSSK family

Cloning, sequence characterization, and expression patterns of members of the porcine TSSK family

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: Developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane

Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197

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