Isolation and characterization of multiple cell types from a single human colonic carcinoma: Tumourigenicity of these cell types in a xenograft system

Abstract

The HCA-7 cell line was established from a moderately well differentiated mucinous adenocarcinoma of the colon, which showed histological heterogeneity. This was reflected in the morphological heterogeneity in early passages of the HCA-7 cell line but diminished as the cells were passaged in vitro. Nine subpopulations were isolated from early passage cultures of the HCA-7 line and maintained as cell lines. Each subpopulation demonstrated a unique set of stable biological characteristics in vitro. When established in vivo, there was a wide variation in xenograft generation time. The parent cell line gave rise to six distinct xenograft patterns, two of which had been observed in the primary carcinoma. Individual subpopulations yielded characteristic tumours which were composed of between one and four organizational patterns. Xenografts differed in both the organization of cells and the cell differentiation within the different patterns. Mucous cells were absent from some tumours while abundant in others. The subpopulations isolated from the HCA-7 cell line provide a new and extensive model system for studying the generation and maintenance of phenotypic heterogeneity in colorectal carcinoma.

Desmethyl Derivatives of Indomethacin and Sulindac as Probes for Cyclooxygenase-Dependent Biology

“…Transient Transfection Assay. The HCA7 human colon cancer cell line was established from a moderately differentiated adenocarcinoma of the colon (14). The cell line was maintained in DMEM supplemented with 100 IU/mL penicillin, 100 g/mL streptomycin, and 10% FBS.…”

“…Indomethacin and 1 were compared for their ability to activate the nuclear transcription factor PPAR␥ in the human colon cancer cell line HCA-7, which basally expresses this nuclear receptor (14,15) Cells were transfected with an expression vector containing luciferase under the control of a PPRE. Addition of either compound triggered a concentration-dependent increase in luciferase activity ( Figure 2, panel a).…”

Type I collagen inhibits differentiation and promotes a stem cell-like phenotype in human colorectal carcinoma cells

“…Three human colorectal cell lines were used in this study: HCA-7 Colony 29 (Marsh et al, 1993) termed Colony 29 in this study, Caco-2 (ECACC) and a twice cloned population of HRA-19 cells (Kirkland, 1988), which have been subjected to multiplex PCR analysis (ECACC; Porton Down, Salisbury, UK) to confirm their unique profile. Cell lines were grown in Dulbecco's Eagle's medium (Invitrogen, Paisley, UK) supplemented with 10% foetal bovine serum (FBS) in 7.5% CO 2 .…”

Comparison of the effects of the chemopreventive agent resveratrol and its synthetic analog trans 3,4,5,4′‐tetramethoxystilbene (DMU‐212) on adenoma development in the ApcMin+ mouse and cyclooxygenase‐2 in human‐derived colon cancer cells

“…HCA-7 cells, derived from a mucinous adenocarcinoma of the colon, 41 and human colon epithelial cells (HCECs) were provided by Drs. S. Kirkland (Imperial College, London, U.K.) and A. Pfeifer (Nestec Research Center, Lausanne, Switzerland), respectively.…”