Isolation and Characterization of Mutants of the Plant Growth-Promoting Rhizobacterium Pseudomonas putida GR12-2 That Overproduce Indoleacetic Acid

Xie, Hui; Pasternak, J.; Glick, Bernard R.

doi:10.1007/s002849900012

Cited by 318 publications

(161 citation statements)

References 22 publications

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“…It has been reported that rhizobacteria induces plant growth by contributing to the host plant endogenous pool of phytohormones, such as IAA (Khalid et al 2004;Patten and Glick 2002). A low level of IAA promotes primary root elongation, whereas a high level stimulates lateral and adventitious root formation (Xie et al 1996). In this study, all strains were able to synthesize IAA in the presence of the precursor Ltryptophan (Fig.…”

Section: Resultsmentioning

confidence: 52%

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Pereira

Barbosa

Castro

2014

Int. J. Environ. Sci. Technol.

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In this work, we evaluated the ability of rhizobacteria isolated from a metal-contaminated site to produce plant growth-promoting substances, such as indole-3-acetic acid (IAA), ammonia, hydrogen cyanide, siderophores and extracellular enzymes, to solubilize phosphate and for 1-aminocyclopropane-1-carboxylate (ACC)-deaminase activity, while assessing their capacity to enhance Trifolium repens growth. The rhizobacteria that better performed were used as bioinoculants for plants grown in zinc-and cadmium-spiked soils. Rhizobacteria were inoculated onto white clover seedlings growing in 250 and 500 mg kg -1 of zinc and in 10 and 30 mg kg -1 of cadmium-spiked soils and plant traits determined. The available and exchangeable rhizosphere metal concentrations were analyzed. Twenty rhizobacteria stimulated white clover growth under axenic conditions. Overall, strains Rhodococcus erythropolis EC 34, Achromobacter sp. 1AP2 and Microbacterium sp. 3ZP2 increased clover biomass in the absence and in presence of metals; however, in soils with 250 mg kg -1 of zinc, bacterial inoculation did not enhance plant biomass. Biomass enhancement may be related to the multiple bacterial promoting traits, especially the production of high levels of IAA and siderophores and the activity of ACC-deaminase. To the best of our knowledge, this is the first study reporting the potential of R. erythropolis to increase plant growth in metal-contaminated soils. In general, strains Microbacterium sp. 3ZP2 and Arthrobacter sp. EC 10 enhanced the available and exchangeable metal concentrations in rhizosphere showing their potential to improve phytoremediation efficiency. This work clearly indicates that rhizobacteria are promising for the development of bioinoculants useful for phytoremediation of polluted soils.

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Section: Resultsmentioning

confidence: 52%

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Pereira

Barbosa

Castro

2014

Int. J. Environ. Sci. Technol.

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“…Further, the IAA produced by PGPB promotes root growth by directly stimulating plant cell elongation or cell division (Glick et al, 1998). A low level of IAA produced by rhizosphere bacteria promotes primary root elongation whereas a high level of IAA stimulates lateral and adventitious root formation but inhibit primary root growth (Xie et al, 1996). Thus PGPB can facilitate plant growth by altering the plant hormonal balance.…”

Section: Discussionmentioning

confidence: 99%

Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

2008

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The metal resistant-plant growth-promoting bacterial (PGPB) strains PsM6 and PjM15 isolated from a serpentine soil were characterized as Pseudomonas sp. and Pseudomonas jessenii, respectively, on the basis of their morphological, physiological, biochemical characteristics and 16S rDNA sequences. Assessment of plant growth-promoting parameters revealed the intrinsic ability of the strains for the utilization of 1-aminocyclopropane-1-carboxylic acid as the sole N source, solubilization of insoluble phosphate and production of indole-3-acetic acid (IAA). Further, a pot experiment was conducted to elucidate the effects of inoculating metal resistant PGPB on the plant growth and the uptake of Ni, Cu and Zn by Ricinus communis. Inoculation of Pseudomonas sp. PsM6 or P. jessenii PjM15 increased the shoot and root biomass of R. communis grown in non-contaminated and contaminated soil. However, the maximum biomass was observed in the plants inoculated with strain PjM15. This effect can be attributed to the solubilization of phosphate and production of IAA. Inoculation of Pseudomonas sp. PsM6 and PjM15 did not greatly alter the organ metal concentrations except Zn which concentration was higher in root, stem and leaf of inoculated plants. The results of metal extraction with PGPB strains showed that PsM6 was more efficient at solubilizing Zn than PjM15, and that PjM15 was better at solubilising Ni and Cu than PsM6. Owing to its wide action spectrum, the metal resistant PGPB could serve as an effective metal sequestering and growth-promoting bioinoculant for plants in metal-stressed soil. The present study has provided a new insight into the phytoremediation of metal-contaminated soil.

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“…As a matter of fact, low levels of IAA produced by rhizobacteria promote primary root elongation, whereas high levels of IAA stimulate lateral and adventitious root formation (Glick, 1995) but inhibit primary root growth (Xie et al, 1996). Thus, plant growth-promoting bacteria can facilitate plant growth by altering the hormonal balance within the affected plant .…”

Section: Rhizobacteria Secretionmentioning

confidence: 99%

Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils

Jing

2007

J. Zhejiang Univ. - Sci. B

403

159

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Heavy metal pollution of soil is a significant environmental problem and has its negative impact on human health and agriculture. Rhizosphere, as an important interface of soil and plant, plays a significant role in phytoremediation of contaminated soil by heavy metals, in which, microbial populations are known to affect heavy metal mobility and availability to the plant through release of chelating agents, acidification, phosphate solubilization and redox changes, and therefore, have potential to enhance phytoremediation processes. Phytoremediation strategies with appropriate heavy metal-adapted rhizobacteria have received more and more attention. This article paper reviews some recent advances in effect and significance of rhizobacteria in phytoremediation of heavy metal contaminated soils. There is also a need to improve our understanding of the mechanisms involved in the transfer and mobilization of heavy metals by rhizobacteria and to conduct research on the selection of microbial isolates from rhizosphere of plants growing on heavy metal contaminated soils for specific restoration programmes.

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Isolation and Characterization of Mutants of the Plant Growth-Promoting Rhizobacterium Pseudomonas putida GR12-2 That Overproduce Indoleacetic Acid

Cited by 318 publications

References 22 publications

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Rhizobacteria isolated from a metal-polluted area enhance plant growth in zinc and cadmium-contaminated soil

Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils

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