Isolation and characterization of new phenolic siderophores with antimicrobial properties from <i>Pseudomonas</i> sp. UIAU-6B

Oluwabusola, Emmanuel T.; Adebisi, Olusoji Olusegun; Reyes, Fernando; Acquah, Kojo Sekyi; Cruz, Mercedes de la; Mweetwa, Larry L; Rajakulendran, Joy Ebenezer; Warner, Digby F.; Deng, Hai; Ebel, Rainer; Jaspars, Marcel

doi:10.3762/bjoc.17.156

Cited by 3 publications

(6 citation statements)

References 46 publications

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“…Measurements of 2 were not possible due to the low quantity of isolated material, but it stands to reason that its configuration is identical to 5 under the assumption of a shared biosynthetic origin. Compound 6 shows an optical rotation of = −10.6, which is consistent with the published value of pseudomonin A ( = −9.5) [ 12 ]. This natural product is structurally closely related to 6 , featuring a terminal amide instead of a carboxylic acid function.…”

Section: Resultssupporting

confidence: 87%

“…Compound 6 represents a possible shunt product of amamistatin biosynthesis. A similar molecule and its putative biosynthetic pathway were recently described by Jaspars et al [ 12 ]. In accordance with this proposal, salicylic acid and ʟ-threonine would form a salimethyloxazolinyl-thioester intermediate, which then undergoes a C–N bond opening resulting in an ester formation.…”

Section: Resultsmentioning

confidence: 70%

“…Previous studies have demonstrated that, in nature, hydroxamates arise from the oxidation of terminal amino groups in amino acid side chains, followed by formylation or acylation of the resulting hydroxylamines. While the oxidations are catalyzed by flavin-dependent monooxygenases [ 12 – 16 ], the carbon transfer is mediated either by tetrahydrofolate-dependent formyl [ 17 ] or by acyl-CoA-dependent acyl transferases [ 13 , 15 – 16 ]. It is widely assumed that the oxidation precedes the formylation or acylation [ 17 – 22 ].…”

Section: Resultsmentioning

confidence: 99%

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Amamistatins isolated from Nocardia altamirensis

Steinmetz¹,

Hiller²,

Nett³

2022

Beilstein J. Org. Chem.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 99%

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Amamistatins isolated from Nocardia altamirensis

Steinmetz¹,

Hiller²,

Nett³

2022

Beilstein J. Org. Chem.

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“…In the isolated Pseudomonas strains, this points to a potential role of this siderophore in the inhibition of the three lettuce bacterial pathogens. Pseudomonine and pseudomonine-like compounds are siderophores identified in various Pseudomonas strains of biocontrol interest ( Mercado-Blanco et al, 2001 ; Loper et al, 2012 ; Biessy et al, 2019 ; Oluwabusola et al, 2021 ). They share structural similarities with pyochelin ( Gross and Loper, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

“…They share structural similarities with pyochelin ( Gross and Loper, 2009 ). Some of these compounds have been shown to inhibit the human bacterial pathogen Mycobacterium tuberculosis in vitro ( Oluwabusola et al, 2021 ). Another family of BGCs found in the genome of the 35 strains under study pertains to NRPS and PKS.…”

Section: Discussionmentioning

confidence: 99%

Harnessing the genomic diversity of Pseudomonas strains against lettuce bacterial pathogens

et al. 2022

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Lettuce is a major vegetable crop worldwide that is affected by numerous bacterial pathogens, including Xanthomonas hortorum pv. vitians, Pseudomonas cichorii, and Pectobacterium carotovorum. Control methods are scarce and not always effective. To develop new and sustainable approaches to contain these pathogens, we screened more than 1,200 plant-associated Pseudomonas strains retrieved from agricultural soils for their in vitro antagonistic capabilities against the three bacterial pathogens under study. Thirty-five Pseudomonas strains significantly inhibited some or all three pathogens. Their genomes were fully sequenced and annotated. These strains belong to the P. fluorescens and P. putida phylogenomic groups and are distributed in at least 27 species, including 15 validly described species. They harbor numerous genes and clusters of genes known to be involved in plant-bacteria interactions, microbial competition, and biocontrol. Strains in the P. putida group displayed on average better inhibition abilities than strains in the P. fluorescens group. They carry genes and biosynthetic clusters mostly absent in the latter strains that are involved in the production of secondary metabolites such as 7-hydroxytropolone, putisolvins, pyochelin, and xantholysin-like and pseudomonine-like compounds. The presence of genes involved in the biosynthesis of type VI secretion systems, tailocins, and hydrogen cyanide also positively correlated with the strains’ overall inhibition abilities observed against the three pathogens. These results show promise for the development of biocontrol products against lettuce bacterial pathogens, provide insights on some of the potential biocontrol mechanisms involved, and contribute to public Pseudomonas genome databases, including quality genome sequences on some poorly represented species.

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Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

Vitorino,

Pinto,

Martín

et al. 2024

Antonie van Leeuwenhoek

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An appealing strategy for finding novel bioactive molecules in Nature consists in exploring underrepresented and -studied microorganisms. Here, we investigated the antimicrobial and tumoral anti-proliferative bioactivities of twenty-three marine and estuarine bacteria of the fascinating phylum Planctomycetota. This was achieved through extraction of compounds produced by the Planctomycetota cultured in oligotrophic medium followed by an antimicrobial screening against ten relevant human pathogens including Gram-positive and Gram-negative bacteria, and fungi. Cytotoxic effects of the extracts were also evaluated against five tumoral cell lines. Moderate to potent activities were obtained against Enterococcus faecalis, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and vancomycin-sensitive and vancomycin-resistant Enterococcus faecium. Anti-fungal effects were observed against Trichophyton rubrum, Candida albicans and Aspergillus fumigatus. The highest cytotoxic effects were observed against human breast, pancreas and melanoma tumoral cell lines. Novipirellula caenicola and Rhodopirellula spp. strains displayed the widest spectrum of bioactivities while Rubinisphaera margarita ICM_H10T affected all Gram-positive bacteria tested. LC-HRMS analysis of the extracts did not reveal the presence of any known bioactive natural product, suggesting that the observed activities are most likely caused by novel molecules, that need identification. In summary, we expanded the scope of planctomycetal species investigated for bioactivities and demonstrated that various strains are promising sources of novel bioactive compounds, which reenforces the potential biotechnological prospects offered by Planctomycetota.

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Isolation and characterization of new phenolic siderophores with antimicrobial properties from Pseudomonas sp. UIAU-6B

Cited by 3 publications

References 46 publications

Amamistatins isolated from Nocardia altamirensis

Amamistatins isolated from Nocardia altamirensis

Harnessing the genomic diversity of Pseudomonas strains against lettuce bacterial pathogens

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

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