Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments

Padua, Jewel C. De; Cruz, Thomas Edison E. dela

doi:10.3390/jof7080591

Cited by 15 publications

(9 citation statements)

References 54 publications

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“…All examined strains exhibited similar tolerance to increased metal concentrations, both from heavily polluted and unpolluted soils ( Table 2 and Table 3 ). Tripathi [ 53 ] demonstrated high tolerance of selected Trichoderma isolates to increased concentrations of nickel, arsenate, and copper in the range of 100 to 250 mg/L. According to our results, T. longibrachiatum could tolerate a Ni concentration of 60 mg/L.…”

Section: Resultssupporting

confidence: 66%

“…strains, which included the dimensions of conidia and phialides, the influence of different concentrations of heavy metals Cu and Ni on their morphology was not observed ( Table S1 ). Good growth of fungi in combination with normal phialides and conidia morphologies is considered an indicator of highly tolerant strains to increased heavy metal concentrations [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

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Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

et al. 2023

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Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

et al. 2023

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“…The success of species belonging to the genus Trichoderma as biocontrol agents in the soil ecosystems results from their ability to rapid growth, the possibility of utilizing a variety of substrates, and resistance to many toxic chemicals, including fungicides (e.g., azoxystrobin, 3,4-dichloroaniline, and trifloxystrobin), herbicides, and other organic pollutants [28,[35][36][37][38]. Other than that, Trichoderma was found to degrade some toxic contaminants through enzymes involved in cellulose/lignin degradation that have been shown to have xenobiotic-metabolizing enzyme potential [36,38].…”

Section: Characteristics Of the Trichoderma Allowing Its Use In Agric...mentioning

confidence: 99%

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

Tyśkiewicz

Nowak

Ozimek

et al. 2022

IJMS

275

136

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Rhizosphere filamentous fungi of the genus Trichoderma, a dominant component of various soil ecosystem mycobiomes, are characterized by the ability to colonize plant roots. Detailed knowledge of the properties of Trichoderma, including metabolic activity and the type of interaction with plants and other microorganisms, can ensure its effective use in agriculture. The growing interest in the application of Trichoderma results from their direct and indirect biocontrol potential against a wide range of soil phytopathogens. They act through various complex mechanisms, such as mycoparasitism, the degradation of pathogen cell walls, competition for nutrients and space, and induction of plant resistance. With the constant exposure of plants to a variety of pathogens, especially filamentous fungi, and the increased resistance of pathogens to chemical pesticides, the main challenge is to develop biological protection alternatives. Among non-pathogenic microorganisms, Trichoderma seems to be the best candidate for use in green technologies due to its wide biofertilization and biostimulatory potential. Most of the species from the genus Trichoderma belong to the plant growth-promoting fungi that produce phytohormones and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme. In the present review, the current status of Trichoderma is gathered, which is especially relevant in plant growth stimulation and the biocontrol of fungal phytopathogens.

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“…The fungus Penicillium glabra series exhibited promising enzymatic activities for protease (720.64 U g −1 ) in 6 h of extraction, with the optimal conditions of pH 4, temperature of 20 °C and agitation of 150 rpm and also at pH 8, temperature of 36 °C and agitation of 150 rpm, presenting 706.29 U g −1 . De Matos 37 found in his study that proteases from fungi species Penicillium have tendencies to optimal results in more alkaline solutions and at lower temperatures. This work obtained satisfactory results at acidic pH (4) and alkaline pH (8).…”

Section: Penicillium Glabra Seriesmentioning

confidence: 99%

Extraction of enzymes produced by endophytic fungi isolated from mangroves

Paliga,

Bonatto,

Camargo

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUNDThis work aimed to study different methodologies for extracting enzymes produced by the endophytic fungi isolated from mangrove Neofusicoccum parvum, Penicillium glabra series and Bjerkandera sp. grown on organic rice. The enzymatic activities of amylase, cellulase, laccase, lipase, peroxidase, protease and keratinase were evaluated for future applications in biotechnology and enzymology. The evaluation of the extraction was carried out using the design of experiments methodology, where variations were made in pH (4–8), temperature (20–36 °C) and agitation (150–200 rpm), in times of 1, 6 and 12 h.RESULTSAmong the enzymes analyzed, promising activities were found for amylase, cellulase, lipase, protease, peroxidase and especially keratinase, presenting activities of up to 7049.47 U g−1 in the fermentation carried out with the fungus Neofusicoccum parvum, 3538.52 U g−1 with the fungus Penicillium glabra series and 5068.20 U g−1 with the fungus Bjerkandera sp. This indicates that the fungi are promising for the production of multienzyme cocktails.CONCLUSIONIt should be emphasized that the microorganisms and extraction conditions evaluated resulted in a series of promising results, that is, in the case of aiming to obtain different enzymes by other extraction conditions, after fermentation of various microorganisms on rice, several options of situations are presented and validated in this work, which allows the choice of the same, depending on the future application potential of these biocatalysts. © 2023 Society of Chemical Industry (SCI).

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Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments

Cited by 15 publications

References 54 publications

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

Extraction of enzymes produced by endophytic fungi isolated from mangroves

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