Isolation and characterization of non-Frankia actinobacteria from root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia

Ghodhbane‐Gtari, Faten; Essoussi, Imene; Chattaoui, Mayssa; Chouaia, Bessem; Jaouani, Atef; Daffonchio, Daniele; Boudabous, Abdellatif; Gtari, Maher

doi:10.1007/s13199-009-0029-7

Cited by 50 publications

(42 citation statements)

References 46 publications

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“…This was demonstrated long back by Fedorov and Kalininskaya (1961) by their ability to grow on N-free medium and acetylene reduction activity. Recent studies using 15 N isotope dilution analysis and identification of nif genes further support this phenomenon (Valdes et al 2005;Ghodhbane-Gtari et al 2010). Molecular studies have established the fact that many actinobacteria can occur as endophytes in various leguminous and non-leguminous plants without forming nodule.…”

Section: Nitrogen Fixationmentioning

confidence: 92%

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

2017

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Grain legumes are a cost-effective alternative for the animal protein in improving the diets of the poor in South-East Asia and Africa. Legumes, through symbiotic nitrogen fixation, meet a major part of their own N demand and partially benefit the following crops of the system by enriching soil. In realization of this sustainability advantage and to promote pulse production, United Nations had declared 2016 as the ''International Year of pulses''. Grain legumes are frequently subjected to both abiotic and biotic stresses resulting in severe yield losses. Global yields of legumes have been stagnant for the past five decades in spite of adopting various conventional and molecular breeding approaches. Furthermore, the increasing costs and negative effects of pesticides and fertilizers for crop production necessitate the use of biological options of crop production and protection. The use of plant growth-promoting (PGP) bacteria for improving soil and plant health has become one of the attractive strategies for developing sustainable agricultural systems due to their eco-friendliness, low production cost and minimizing consumption of non-renewable resources. This review emphasizes on how the PGP actinobacteria and their metabolites can be used effectively in enhancing the yield and controlling the pests and pathogens of grain legumes.

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Section: Nitrogen Fixationmentioning

confidence: 92%

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

2017

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“…While the ability to grow on nitrogen-free media is used to demonstrate atmospheric nitrogen fixation by bacteria, this line of reasoning may not be true for slowly growing actinobacteria (Ghodhbane- Gtari et al 2010). It is difficult to obtain completely nitrogen-free media that is not contaminated by trace amounts of combined nitrogen in distilled water and other chemical compounds and/or glassware in a routine microbiological laboratory.…”

Section: Conclusion and Prospectivementioning

confidence: 99%

Phylogenetic perspectives of nitrogen-fixing actinobacteria

et al. 2011

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It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. During the last decade, Mycobacterium flavum, Corynebacterium autotrophicum and a fluorescent Arthrobacter sp. have been reported to have nitrogenase activity, but these studies have not been further verified. Additional reports of nitrogen fixation by Agromyces, Microbacterium, Corynebacterium and Micromonospora isolated from root nodules of leguminous and actinorhizal plants have increased. For several actinobacteria, nitrogen fixation was demonstrated by the ability to grow on nitrogen-free medium, acetylene reduction activity, 15N isotope dilution analysis and identification of a nifH gene via PCR amplification. Moreover, the analyses of draft genome sequences of actinobacteria including Slackia exigua, Rothia mucilaginosa and Gordonibacter pamelaeae have also revealed the presence of nifH-like sequences. Whether these nifH sequences are associated with effective nitrogen fixation in these actinobacteria taxa has not yet been demonstrated. These genes may be vertically or horizontally transferred and be silent sequences. These ideas merit further investigation. This minireview presents a phylogenetic comparison of nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of this catalytic ability among actinobacteria.

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“…In addition, Carro et al (2013) found that Micromonospora strains are ubiquitous in actinorhizal nodules and Micromonospora strains with nitrogenase activity were isolated from nitrogen-fixing root nodules of actinorhizal plants (Niner et al 1996;Valdés et al 2005;Trujillo et al 2006a). This work and others demonstrated the existence in root nodules of non-Frankia symbionts that harbors nitrogenase gene and fix nitrogen (Valdés et al 2005;Gtari et al 2007; Ghodhbane- Gtari et al 2010). Carro et al (2013) stated that Micromonospora isolates are normal occupant of actinorhizal nodules but their function is unknown at the moment.…”

Section: Symbiotic Actinobacteria: Genus Frankiamentioning

confidence: 94%

“…However, the in situ role of endophytic Actinobacteria to the plant life is relative poorly understood although some works reported relationship between their presence and resistance to various biotic and abiotic stress such as light deficiency, diseases (Sessitsch et al 2002), drought (Hasegawa et al 2004) or as PGP that produce IAA (El-Tarabily et al 2009;Ghodhbane-Gtari et al 2010;Nimnoi et al 2010), pteridic acids (Igarashi et al 2002) or indole-3-pyruvic acid (IPYA) (ElTarabily et al (2009). Association of endophytic Actinobacteria with Rhizobiumlegume symbionts was also reported.…”

Section: Endophytic Actinobacteriamentioning

confidence: 99%

Diversity of Plant Associated Actinobacteria

Bouizgarne

Aouamar

2014

Sustainable Development and Biodiversity

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The phylum Actinobacteria encompasses Gram-positive bacteria with a high DNA G+C. In soils, they present multiple lifestyles: rhizosphere saprophytes, endophytes, facultative symbionts and obligate phytopathogens. They play either beneficial or adverse effects towards plants. Numerous studies focused on their taxonomy and preservation. Also, adequate strategies were developed to enable their isolation. Phenotypically, the Actinobacteria is one of the most diverse phyla within bacteria. Their presence was once monitored by classical culture dependent techniques and phenetic characterization. Development of culture independent methods including chemotaxonomic and genomic approaches such as 16S rRNA gene analysis allowed to detect the so called unculturable bacteria. Major works on their diversity combine culture dependant and independant techniques. This chapter focuses on the phenetic and genetic diversity of Actinobacteria in plant-soil systems. It begins with some knowledge of their biology and their taxonomy, followed by a brief overview of the methods that have facilitated advances in the understanding of their diversity. It also discusses diversity of ecologically important plant associated Actinobacteria (rhizosphere and phyllosphere colonizers, endophytes, symbionts and phytopathogens).

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Isolation and characterization of non-Frankia actinobacteria from root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia

Cited by 50 publications

References 46 publications

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes

Phylogenetic perspectives of nitrogen-fixing actinobacteria

Diversity of Plant Associated Actinobacteria

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