Isolation and characterization of novel EST-SSRs in the showy dendrobium, Dendrobium nobile (Orchidaceae)

Abstract

ABSTRACT. Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers could enrich the current resource of molecular markers. In this study, microsatellite markers were developed for Dendrobium nobile Lindl. by mining the ESTs. Twenty-eight ESTSSRs amplified 2 to 6 nucleotide repeats with a mean number of 2.82 alleles per locus. The observed and expected heterozygosities per locus ranged from 0.158 to 0.579 and 0.422 to 0.752, respectively. These novel EST-SSRs enriched the current resource of molecular markers for the Dendrobium genus and would facilitate further applications in germplasm appraisal, evolution and genetic diversity studies, genetic mapping, and plant breeding of D. nobile and other congeneric species.

Quantitative analysis of six sesquiterpene glycosides from Dendrobium nobile Lindl. under different growth conditions by high‐performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry in MRM mode

“…According to the previous research of our group 36 and the present data analysis, the contents of SGs from D. nobile may be correlated with altitude. To visualize our findings, we performed HCA on six SGs from D. nobile grown in different environments.…”

“…According to the previous research of our group 36 At present, studies have shown that the accumulation of secondary metabolites is closely related to the environment. 37 Different attachment methods (rock wall, pear tree, greenhouse potted plant) affect the secondary metabolite contents of D. officinale.…”

Analysis of microsatellites in the vulnerable orchid Gastrodia flavilabella: the development of microsatellite markers, and cross-species amplification in Gastrodia

“…The genetic diversity, including the means of the observed ( Ho ) and expected heterozygosity ( He ) (Table 4), of G. flabilabella was low compared with that of other Orchidaceae species, such as Dendrobium huoshanense (0.512 and 0.569) (Wang et al [2012b]), Dendrobium officinale (0.720 and 0.740) (Xie et al [2010]), Dendrobium officinale (0.514 for Ho ) (Lu et al [2012]), and Dendrobium nobile (0.350 and 0.608) (Lu et al [2014]). Unfortunately, no data for any Gastrodia taxa or mycoheterotrophic orchids are available for the comparison of genetic variability.…”

Development and Characterization of Expressed Sequence-tagged Simple Sequence Repeat Markers for Denphal-type Dendrobium Cultivars and Transferability to Dendrobium Species

“…Simple sequence repeat (SSR) markers have been used for germplasm management and marker-assisted selection of plants because of their advantages of being highly polymorphic, highly reproducible, and readily transferable to other populations within the same species and related species and genera (Kang et al 2015;Lu et al 2013;Tsai et al 2015). Recently, a more informative co-dominant type of marker (i.e., SSRs) has been developed to identify Dendrobium and genetic linkage maps of Dendrobium (Liu et al 2014;Lu et al 2012a;Lu et al 2012b;Lu et al 2014. However, these studies were most focused on medical Dendrobium and Nobile-type Dendrobium plants.…”