Isolation and characterization of novel microsatellite markers for molecular genetic diversity in Siganus fuscescens

Abstract

ABSTRACT. The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats. Polymorphisms in these 10 microsatellite markers were determined from 32 wild individuals. The number of alleles per locus and the polymorphism information content ranged from 2 to 5 and from 0.059 to 0.668, respectively. The observed and expected heterozygosities varied from 0.063 to 0.781 and from 0.062 to 0.731, respectively. Although 1 locus (LZY-X7, P < 0.005) showed significant deviation from the Hardy-Weinberg equilibrium, no deviations were detected in the other 9 loci. These microsatellite loci may be useful for further population genetic studies, conservation studies, population structure assessment, and linkage map construction of S. fuscescens.

Screening and characterization of novel microsatellite loci in Lateolabrax japonicus

“…For these reasons, they have been the genetic marker of choice for studies of population and evolutionary genetics; population differentiation (Li, 2006); and genome mapping and forensic studies (Goldstein and Schlötterer, 1999). The use of microsatellite markers has been reported in many fishes such as Oncorhynchus mykiss (Jackson, 1995), Oreochromis niloticus (Lee and Kocher, 1996), Gadus morhua (Herbinger et al, 1997), Carassius gibelio (Zhou et al, 2001), Siganus fuscescens (Ning et al, 2015), and Coilia nasus (Fang et al, 2015). There are also examples of studies of L. japonicus using microsatellite markers (Zhu and Chang, 1999;Jiang et al, 2007Jiang et al, , 2008Jiang et al, , 2009Liu et al, 2009;Zhao et al, 2011).…”

Isolation and characterization of new microsatellite markers in red tail prawn, Fenneropenaeus penicillatus, an endangered species in China

“…They can be used to assess genetic variability and inbreeding, parentage assignment, species and strain identification, hybridization, and marker-assisted identification of quantitative trait loci through the construction of genetic linkage maps (Liu and Cordes, 2004;Dawson et al, 2013). Microsatellite markers are widely used in the study of wild and cultured aquatic species such as Sebastiscus marmoratus , Branchiostoma belcheri Gray , and Siganus fuscescens (Ning et al, 2015). Eighteen polymorphic microsatellite loci of F. penicillatus have been developed for genetic conservation (Cao et al, 2012;Shangguan et al, 2014).…”

Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii

“…Thirty genomic DNA samples were used to confirm the conditions for each primer pair. The total volume of the reaction mix for PCR was 10 µL, and cycling parameters were set according to the following conditions: a) 94°C for 10 min to ensure the DNA was denatured; b) 28-32 cycles for PCR amplification acted as 94°C for 40s, annealing temperature (Table 1) for 40 s, 60s at 72°C, for extension; c) 72°C for 10 min (Ning et al, 2015). Finally, polymorphism was acknowledged by the Sequi-Gen Sequencing Cell machine (Bio-Rad, Hercules, CA, USA) using 6% denaturing polyacrylamide gels and, ultimately, makes it visible through the silver staining.…”