Isolation and characterization of novel Streptomyces strain from Algeria and its in-vitro antimicrobial properties against microbial pathogens

Djebbah, Fatima Zohra; Belyagoubi, Larbi; Abdelouahid, Djamel Eddine; Kherbouche, Farid; Al-Dhabi, Naïf Abdullah; Arasu, Mariadhas Valan; Ravindran, Balasubramani

doi:10.1016/j.jiph.2021.09.019

Cited by 16 publications

(6 citation statements)

References 25 publications

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“…The sporophore nature of the selected potential isolate was observed in the Scanning Electron Microscope (SEM). The optimum culture conditions for maximum production of active compound was determined by using various ranges of temperature (4-50EC), pH (4-10), incubation period and salinity (1-32%) on different culture media, including ISP (1-7) media recommended by the International Streptomyces Project (ISP) and other standard procedures 10 .…”

Section: Phenotypic Characterization and Optimization Of Cultural Con...mentioning

confidence: 99%

Antimicrobial Efficiency of a Marine Streptomyces sp. VPTSA1-4 Isolated from Coromandel Coast of Bay of Bengal, India

Vijayakumar¹,

Raja²,

Thirumurugan³

et al. 2023

J. of Applied Sciences

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Background and Objective: The need and search for therapeutic agents in the treatment of existing and emerging microbial diseases have intensified. The ever-reliable marine streptomycetes have been less explored for such antimicrobial agents and the present study is an attempt in that direction, wherein actinobacteria from marine sources are assessed for their antimicrobial activity. Materials and Methods: The actinobacteria isolated from coastal soils were screened for antimicrobial activity against microbial pathogens. Streptomycetes with the best activity were identified by 16S rRNA gene sequencing. The bioactive compound was extracted and subjected to spectral studies. Mean values of the data derived in antimicrobial efficacy were statistically analyzed. Results: Out of 19 isolates, Streptomyces sp., VPTSA1-4 exhibited the best antimicrobial activity and hence, 16S rRNA genome was sequenced to ascertain the identity of the isolate produced maximum antimicrobial compound at 30EC on the 9th day with a pH of 8 and salinity of 4%, on a starch-casein medium. Further, the potential isolate was identified as Streptomyces tuirus VPTSA1. Conclusion: The study suggested that antimicrobial compounds from Streptomyces tuirus could be used as alternative antimicrobial drugs to drug-resistant pathogens.

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Section: Phenotypic Characterization and Optimization Of Cultural Con...mentioning

confidence: 99%

Antimicrobial Efficiency of a Marine Streptomyces sp. VPTSA1-4 Isolated from Coromandel Coast of Bay of Bengal, India

Vijayakumar¹,

Raja²,

Thirumurugan³

et al. 2023

J. of Applied Sciences

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“…Soil samples from Kerala were acquired, sealed in sterile Ziploc bags, and transported to the laboratory. They were air-dried and suspended in sterile distilled water (1 g/100 mL), homogenized by vortexing, and 0.1 mL of the serially diluted sample of 10 -5 dilution was pour plated on the International Streptomyces Project 4 (ISP-4) agar for isolation of Streptomyces [16]. Post incubation, the antimicrobial activity of the isolate was recorded by observing the inhibition zone around the inoculated spot with the isolated strains.…”

Section: Isolation Of Streptomyces Strain and Preliminary Antimicrobi...mentioning

confidence: 99%

Streptomyces peucetius M1 and Streptomyces lavendulae M3 Soil Isolates as a Promising Source for Antimicrobials Discovery

Jha¹,

Jain²,

Nikumb³

et al. 2022

JPRI

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The ever-increasing fatality due to the surge of drug resistance has demanded the development of therapeutic metabolites with novel modes of mechanism. Despite the discovery of new antimicrobials, antibiotic resistance is increasing at an alarming rate. The emergence of novel molecules is necessary for targeting the Multidrug (MDR) and Pan-Drug resistant (PDR) pathogens which are the principal cause of life-threatening infections. Streptomyces strains commonly found in the soil produce secondary metabolites which have outstanding potential for being an antifungal and antibacterial agent. This study aims to isolate, identify, and characterize Streptomyces strains isolated from soil and extract their crude metabolites to determine their hidden therapeutic property. Out of four isolates identified, Streptomyces peucetius and Streptomyces lavendulae have shown significant antimicrobial activity against test organisms. To further determine the activity of their extracts GC-MS analysis, and MIC, as well as radical scavenging activity, were determined. GC-MS analysis revealed the presence of compounds 2,3-butanediol, 1-methyl-2-pyrrolidone-4-carboxamide, and L-Lactic acid which are frequently associated with potent antimicrobial nature. The most vulnerable pathogen to M1 extract was Candida albicans with a MIC value of 7.8125 μg/mL while the susceptible bacteria to M3 extract included Staphylococcus aureus with a MIC value of 3.90 μg/mL. The data obtained for antioxidant activity in the case of metabolic extracts M1 and M3 had an IC50 value of 43.17 µg/mL and 22.65 µg/mL respectively. The above findings hint at the unrealized potential of Streptomyces strains.

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“…The morphology of the colonies was elevated with wrinkled surface and solid density was similar to other studies on Streptomyces sp. (Djebbah et al 2021). Streptomyces is characterized by dry, tiny compact, soft to powdery colonies constantly attached to the medium forming aerial and substrate mycelium with an earthy smell (Sathi et al 2001;Yanti et al 2019).…”

Section: Desizingmentioning

confidence: 99%

Partial Purification of Extracellular Amylase From Halotolerant Actinomycetes Streptomyces brasiliensis MML2028

Abirami¹,

Ramprasath²,

Suganthi³

et al. 2022

J Exp Bio & Ag Sci

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Amylase is considered as an industrially important enzyme as it occupies the most important function in the food, paper, and pharmaceutical industries. The present study is concerned with the optimization, production and partial purification of halotolerant amylase from newly isolated Streptomyces brasiliensis MML2028, from Kelambakkam salt pan, Tamil Nadu, India. The primary screening was carried out by well diffusion assay to find the zone of lysis. The assay was observed for each media optimization by measuring the release of reducing sugar (RS) by the 3,5 dinitro salicylic acid (DNS) method and expressed in the international unit (UI). Ammonium sulphate precipitation was used to partially purify the enzyme and then lyophilized. SDS-PAGE was performed to identify the molecular weight. The production medium was optimized with 1% of the starch substrate, 3% of NaCl at 24˚C and pH 9, and incubation of 9 days. The total activity of the partially purified α-amylase was observed to be 1806.9U/mL. The partially purified enzyme was more active with 3% NaCl, pH 8, and 24˚C which is known to be a halotolerant alkaline α-amylase. The enzyme showed tolerance towards magnesium, manganese ions, Triton x-100, and urea. De-inking of α-amylase showed good results proving that the enzyme activity is more efficient. Hence, the alkaliphilic amylase from Halotolerant actinomycetes S. Brasiliensis MML2028 could be a better microbial source that can be used in many industries, especially in paper and textiles.

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Isolation and characterization of novel Streptomyces strain from Algeria and its in-vitro antimicrobial properties against microbial pathogens

Cited by 16 publications

References 25 publications

Antimicrobial Efficiency of a Marine Streptomyces sp. VPTSA1-4 Isolated from Coromandel Coast of Bay of Bengal, India

Antimicrobial Efficiency of a Marine Streptomyces sp. VPTSA1-4 Isolated from Coromandel Coast of Bay of Bengal, India

Streptomyces peucetius M1 and Streptomyces lavendulae M3 Soil Isolates as a Promising Source for Antimicrobials Discovery

Partial Purification of Extracellular Amylase From Halotolerant Actinomycetes Streptomyces brasiliensis MML2028

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