ISOLATION AND CHARACTERIZATION OF NUTRITIONALLY DEFICIENT CELLS FROM CULTURED CHINESE HAMSTER &lt;i&gt;HAI&lt;/i&gt; CELLS BY A REPLICA PLATING METHOD

Suzuki, Fumio; Horikawa, Masakatsu

doi:10.1266/jjg.50.247

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…The origin, culture procedures and properties of the Chinese hamster hai cell line used in the present study have been described previously (Suzuki and Horikawa 1973). Alanine-, asparagine-, proline-, aspartic acid-, hypoxanthine-, and glutamic acid-prototrophic CH-hai Cl 23 cells (Suzuki and Horikawa 1975;Ban et al 1976) isolated from the cultures of Chinese hamster hai cells by a replica plating method were used as 8AG-sensitive cells. In this study, however, CH-hai C123 cells were cultured in a medium composed of 90% Eagle's MEM+N17 medium (which lacked 1) Present address:…”

Section: Methodsmentioning

confidence: 99%

Studies on Somatic Cell Mutations

Horikawa

Suzuki

Ban

1976

The Japanese journal of genetics

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It is very importantto establish the sensitive assay systems for the mutations using mammalian cells in vitro for detecting various potential mutagens present in the human environment.These systems are also useful for studying the mechanisms of somatic mutation (involving the problems of phenotypic expression, repair of premutational damage, specific stage in the cell cycle and so on) in cultured mammalian cells. We found in the previous study (Ban et al. 1976) that the forward mutation system using prototrophic CH-hai C123 cells isolated from an original Chinese hamster hai cell line is much more sensitive than the reverse mutation system using auxotrophic CH-hai Cl 3 cells for detecting the mutations induced by radiations and chemical carcinogens or mutagens.In the present study, as a series of these experiments, we examined the frequencies of the forward and reverse mutations induced by X-rays and UV, using 8-azaguanine (8AG)-sensitive cells (the prototrophic CH-hai C123 cells) and 8AG-resistan cells which are isolated from them, and compared the sensitivities as a mutation assay system with those of the nutritionally forward and reverse mutations used previously (Ban et al. 1976) . Contrary to expectation, the results obtained in this study were rather negative as far as mutation induction are concerned. MATERIALS AND METHODSCells and medium. The origin, culture procedures and properties of the Chinese hamster hai cell line used in the present study have been described previously (Suzuki and Horikawa 1973). Alanine-, asparagine-, proline-, aspartic acid-, hypoxanthine-, and glutamic acid-prototrophic CH-hai Cl 23 cells (Suzuki and Horikawa 1975;Ban et al. 1976) isolated from the cultures of Chinese hamster hai cells by a replica plating method were used as 8AG-sensitive cells. In this study, however, CH-hai C123 cells were cultured in a medium composed of 90% Eagle's MEM+N17 medium (which lacked 1) Present address:

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Section: Methodsmentioning

confidence: 99%

Studies on Somatic Cell Mutations

Horikawa

Suzuki

Ban

1976

The Japanese journal of genetics

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“…Alanine-, asparagine-, proline-, aspartic acid-, and hypoxanthine-prototrophic clone (CH-hai Cl 23 cells (Suzuki and Horikawa 1975)) and thymidine-auxotrophic clone (CHhai Cl 3 cells (Suzuki and Horikawa 1975)) isolated from an original Chinese hamster hai cells by our replica plating method (Suzuki and Horikawa 1973) were used in the present studies.…”

Section: Cells and Mediummentioning

confidence: 99%

“…The culture procedures and properties of these clones have been described previously (Suzuki and Horikawa 1975). Briefly, auxotrophic CH-hai Cl 3 cells were maintained in a complete medium composed of 90% Eagle's MEM+N18 medium (Suzuki and Horikawa 1973) and 10% dialyzed calf serum (Suzuki and Horikawa 1973) after the isolation.…”

Section: Cells and Mediummentioning

confidence: 99%

“…Additionally, thymidine-prototrophic CH-hai Cl 7 cells (colone 7 in Table 2 (Suzuki and Horikawa 1975)) isolated from the original Chinese hamster hai cells by the replica plating method were used as a control for the assay of thymidylate synthetase activity. These cells were maintained in a medium composed of 90% Eagle's MEM +N17 medium (which lacked thymidine from Eagle's MEM+ N18 medium) and 10% dialyzed calf serum.…”

Section: Cells and Mediummentioning

confidence: 99%

“…In the present study, we examined the frequency of the forward and reverse mutations induced by radiations (X-rays and UV) and chemicals (4-nitroquinoline 1-oxide, N methyl-N'-nitro-N nitrosoguanidine, ethyl methanesulf onate), by using prototrophic CH-hai C123 cells and auxotrophic CH-hai Cl 3 cells (Suzuki and Horikawa 1975) isolated from an original Chinese hamster hai cell line, and compared the sensitivity as the assay systems for mutagens.…”

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Studies on Somatic Cell Mutations

Horikawa

Suzuki

Ban

1976

The Japanese journal of genetics

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Isolation of UV‐sensitive clones from mouse cell lines by lederberg style replica plating

Kuroki

Miyashita

1977

Journal Cellular Physiology

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Seven UV-sensitive clones were isolated from the mouse cell lines, FM3A and L5178Y, using Lederberg style replica plating. The UV-sensitive clones were found at a frequency of 1 in 886 clones of FM3A and of 6 in 420 clones of L5178Y, after treatment with N-methyl-N'-nitro-N-nitrosoguanidine, but no UV-sensitive clones were obtained in the untreated controls. The Do values of freshly isolated clones were 1 0 to 2 1 ergs/mmZ (mainly 10 to 13 ergs/ mmz), while the original FM3A and L5178Y lines had Do values of 32 ergs/mm2. The UV-sensitive clones were found to be rather stable for a period ranging from two to eight months after isolation. However, later some of them tended to lose their UV-sensitivity, in terms of increase in Do values or n values. More stable UV-sensitive subclones could be isolated from these populations by cloning using the replica plating method. Caffeine was found to potentiate the lethal action of UV-irradiation on the UV-sensitive clones as well as on the original FM3A cells.

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ISOLATION AND CHARACTERIZATION OF NUTRITIONALLY DEFICIENT CELLS FROM CULTURED CHINESE HAMSTER <i>HAI</i> CELLS BY A REPLICA PLATING METHOD

Cited by 6 publications

References 19 publications

Studies on Somatic Cell Mutations

Studies on Somatic Cell Mutations

Studies on Somatic Cell Mutations

Isolation of UV‐sensitive clones from mouse cell lines by lederberg style replica plating

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