Isolation and Characterization of Phenol-Degrading Soil Bacterium<i>Xanthobacter flavus</i>

Lowry, Madan; Nagamani, A.; Kuruva, Sreenivasulu; Soligalla, Rupadevi

doi:10.1080/10889860802686388

Cited by 10 publications

(2 citation statements)

References 21 publications

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“…It is interesting to note that higher phenol concentrations had a negative effect on the growth of the bacterium. This could be due to several factors, such as toxicity or inhibitory effects of accumulated metabolic by-products [ 31 ]. However, Nouri et al reported a strain of Trichosporon cutaneum that can remove phenol with an efficiency of 99% at a concentration of 1250 mg/L [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation

Mirdamadian,

Asad,

Dastgheib

et al. 2024

BMC Microbiol

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The present study aimed to develop a system using a combination of enzymatic and microbial degradation techniques for removing phenol from contaminated water. In our prior research, the HRP enzyme extracted from horseradish roots was utilized within a core-shell microcapsule to reduce phenolic shock, serving as a monolayer column. To complete the phenol removal process, a second column containing degrading microorganisms was added to the last column in this research. Phenol-degrading bacteria were isolated from different microbial sources on a phenolic base medium. Additionally, encapsulated calcium peroxide nanoparticles were used to provide dissolved oxygen for the microbial population. Results showed that the both isolated strains, WC1 and CC1, were able to completely remove phenol from the contaminated influent water the range within 5 to 7 days, respectively. Molecular identification showed 99.8% similarity for WC1 isolate to Stenotrophomonas rizophila strain e-p10 and 99.9% similarity for CC1 isolate to Bacillus cereus strain IAM 12,605. The results also indicated that columns using activated sludge as a microbial source had the highest removal rate, with the microbial biofilm completely removing 100% of the 100 mg/L phenol concentration in contaminated influent water after 40 days. Finally, the concurrent use of core-shell microcapsules containing enzymes and capsules containing Stenotrophomonas sp. WC1 strain in two continuous column reactors was able to completely remove phenol from polluted water with a concentration of 500 mg/L for a period of 20 days. The results suggest that a combination of enzymatic and microbial degrading systems can be used as a new system to remove phenol from polluted streams with higher concentrations of phenol by eliminating the shock of phenol on the microbial population.

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Section: Resultsmentioning

confidence: 99%

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation

Mirdamadian,

Asad,

Dastgheib

et al. 2024

BMC Microbiol

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“…(Zhai et al, 2012), Acinetobacter sp. (Ahmad et al, 2011), Bacillus cereus (Banerjee and Ghoshal, 2010), Staphylococcus epidermis (Mohite et al, 2010), Xanthobacter flavus (Lowry et al, 2009), Burkholderia cepacia (El-Sayed et al, 2003), Pseudomonas (Mahiudddin et al, 2012;Ahmad et al, 2014). Therefore, the aim of this work was to isolate and characterize phenol degrading bacteria from wastewater.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Phenol degrading Bacteria from Wastewater

Mousa

2023

IJBPCS

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Out of 30 bacterial isolates from wastewater were checked for growth on a minimal salt medium amended with different concentrations of phenol by flask culture technique. The eight most tolerant bacterial strains to the higher concentrations of phenol, designated as W2, W5, W9, W12, W14, W15, W19 and W29, were investigated for their ability to grow and degrade phenol. Among the eight higher phenol degrading isolates, W15 can tolerate up to 1000 ppm of phenol concentrations and grow and degrade 94% of phenol within 72 hrs. The optimum temperature and pH condition were 37C◦ and 7, respectively. The yeast extract is the best organic nitrogen source, while ammonium chloride is the best inorganic nitrogen source for the growth and degradation of phenol.

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Efficacy of Acinetobacter sp. B9 for simultaneous removal of phenol and hexavalent chromium from co-contaminated system

Bhattacharya

Gupta

Kaur

et al. 2014

Appl Microbiol Biotechnol

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The present study shows the feasibility of a newly isolated strain Acinetobacter sp. B9 for concurrent removal of phenol and Cr (VI) from wastewater. The experiments were conducted in a batch reactor under aerobic conditions. Initially, when mineral salt solution was used as the culture medium, the strain was found to utilize phenol as sole carbon and energy source while no Cr (VI) removal was observed. However, the addition of glucose as co-carbon source resulted in the removal of both toxicants. This co-removal efficiency of the strain was further improved with nutrient-rich media (NB). Optimum co-removal was determined at 188 mg L(-1) of phenol and 3.5 mg L(-1) of Cr (VI) concentrations at pH 7.0. Strain B9 followed the orthometabolic pathway for phenol degradation. Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR) studies showed sorption of chromium as one of the major mechanisms for Cr (VI) removal by B9 cells. Acinetobacter sp. B9 was later on checked for bioremediation of real tannery wastewater. After 96 h of batch treatment of tannery effluent containing an initial 47 mg L(-1) phenol and 16 mg L(-1) Cr (VI), complete removal of phenol and 87 % reduction of Cr (VI) were attained, showing high efficiency of the bacterial strain for potential application in industrial pollution control.

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Isolation and Characterization of Phenol-Degrading Soil BacteriumXanthobacter flavus

Cited by 10 publications

References 21 publications

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation

Isolation and Characterization of Phenol degrading Bacteria from Wastewater

Efficacy of Acinetobacter sp. B9 for simultaneous removal of phenol and hexavalent chromium from co-contaminated system

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