Isolation and characterization of phenol degrading bacteria immobilized onto cyclodextrin-hydrogel particles within a draft tube spouted bed bioreactor

Safont, Bárbara; Vitas, Ana Isabel; Peñas, Francisco Javier

doi:10.1016/j.bej.2012.03.005

Cited by 16 publications

(7 citation statements)

References 37 publications

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“…The solid phase consisted of different batches (100 or 200 mL) of monodisperse size particles of a cyclodextrin‐based polymer (CDP) or glass beads. The solid holdup values employed here were in agreement with those used in earlier studies with CDP bioparticles 6, 9, 10. The range of the experimental conditions used in the oxygenation experiments is summarized in Tab.…”

Section: Methodssupporting

confidence: 79%

“…On the other hand, the presence of the draft tube allows better bed circulation with lower energy requirements but with poor aeration. Indeed, in spite of its good performance, the maximum elimination capacity reached with this system was only 2.8 kg phenol m −3 d −1 and was limited by the oxygen transfer to the bulk liquor 6.…”

Section: Introductionmentioning

confidence: 94%

“…In a previous work, we studied the removal of aqueous phenol in a draft‐tube spouted‐bed (DTSB) bioreactor loaded with beads of a cyclodextrin‐based support inoculated with a mixed culture 6. Briefly, in a DTSB reactor, solid particles are lifted through the central tube by the rising fluid and drop towards the annular region 7.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Modeling of Oxygen Transfer in a Spouted‐Bed Reactor with Auxiliary Aeration

Peñas

Safont

2016

Chem Eng & Technol

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A draft-tube spouted-bed (DTSB) reactor was equipped with an auxiliary aeration device to provide air into the annular region and thereby improve the oxygen transfer efficiency. The effects of the total air flow rate and its distribution between spout and annulus, the liquid phase viscosity (water and carboxymethyl cellulose solutions), and the solid holdup (glass and cyclodextrin polymer beads) on the oxygen transfer efficiency were discussed. The oxygen transfer coefficient increased with the air flow rate and the ratio of air flowing through the annulus, whereas it decreased with increasing viscosity and solid holdup. A correlation was proposed to predict the transfer coefficient in DTSB reactors with primary and auxiliary aeration. A good fitting was achieved between the experimental data and those estimated with the model.

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Section: Methodssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 94%

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Characterization and Modeling of Oxygen Transfer in a Spouted‐Bed Reactor with Auxiliary Aeration

Peñas

Safont

2016

Chem Eng & Technol

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“…According to a preliminary GC-MS identification, the likely possibility of the odd peak would be an aromatic material, dimethyl phthalate, or vitamin A aldehyde, which could be a bacterial enzyme released at those conditions. However, it has been pointed out by several studies (Chung et al, 2003;Lu et al, 1996;Reardon et al, 2000;Annadurai et al, 2002;Safont et al, 2012) that the formation of dead end metabolic intermediates or the toxic effect of the substrate on the cells is often associated with a yellowish to brownish colour change, often resulting from involving the meta-pathway for degradation, which is more related to incomplete mineralization (Solyanikova and Golovleva, 2004;Lu et al, 1996). Such a colour change has never been detected in any situation in the present study.…”

Section: Effect Of Temperaturecontrasting

confidence: 55%

Biodegradation of 2, 4 Dichlorophenol

Al-Khalid¹,

El‐Naas²

2017

American Journal of Engineering and Applied Sciences

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Abstract:The present study focuses on the optimization of process parameters of the aerobic biodegradation of 2, 4 Dichlorophenol (2, 4 DCP) by a commercial strain of P. putida immobilized in PVA gel matrix, using design of experiments by Response Surface Methodology (RSM). The degradation rates obtained from these experiments were used to evaluate the effects of the main factors and their interactions during the biodegradation of 2, 4 DCP. An effective quadratic regression model predicting the rate in terms of the main independent variables, namely temperature, initial pH and initial concentration of 2, 4 DCP, was developed. The optimum conditions for DCP degradation were obtained as follows: Temperature 32.6°C, pH 5.0 and initial DCP concentration 70.5 mg L −1 , resulting in a maximum predicted degradation rate of 41.8 mg L. Under these optimized conditions, a degradation rate of 40.1 mg L −1 h −1was experimentally obtained, thus validating the model.

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“…Similarly, Sevillano et al used β-CD hydrogels as bacterial supports in a fluidized bed reactor system (Sevillano et al, 2008 ). More recently, Safont et al ( 2012 ) have used β-CD polymer hydrogels to immobilize phenol-degrading microorganisms. Thus, cross-linked CDs offer a potential alternative as dual adsorbent/immobilization materials for biotechnology and environmental remediation ( cf .…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

2018

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Dual function polymer materials with immobilized Sphingobium Chorophenolicum (SpC) bacterium cells are reported herein that undergo tandem adsorption and biodegradation of phenolic compounds. The cross-linked polymer materials contain β-cyclodextrin (β-CD) with incremental hexamethylene diisocyanate (HDI) cross-linker at variable mole ratios (X = 1, 3, or 6), denoted as HDI-X systems. The adsorptive uptake properties of the insoluble HDI-X polymers (X = 3 and 6) with various phenolic compounds [pentachlorophenol (PCP), 2,4,6-trichlorophenol (TCP), and 2,4,6-trimethylphenol (TMP)] were studied using batch adsorption isotherms. The molecular selective phenol removal (SR) capacity of the HDI-3 and HDI-6 materials was evaluated by electrospray ionization mass spectrometry (ESI-MS). The results were compared against granular activated carbon (GAC) and native β-CD, where 1D/2D 1H NMR spectral characterization of the complexes formed between phenolic guests and a soluble polymer (HDI-1) in aqueous solution provide insight on the intermolecular interactions and the role of cross-linking effects. Immobilization of SpC onto HDI-3 was shown to form a composite polymer/bacterium material. The composite system displays synergistic removal effects due to tandem PCP adsorption and SpC biodegradation to yield by-products such as 2,6-dichloro-1,4-hydroquinone (DCHQ). Apoptosis and cytotoxicity of DCHQ were evaluated using three breast cancer cell lines.

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Isolation and characterization of phenol degrading bacteria immobilized onto cyclodextrin-hydrogel particles within a draft tube spouted bed bioreactor

Cited by 16 publications

References 37 publications

Characterization and Modeling of Oxygen Transfer in a Spouted‐Bed Reactor with Auxiliary Aeration

Characterization and Modeling of Oxygen Transfer in a Spouted‐Bed Reactor with Auxiliary Aeration

Biodegradation of 2, 4 Dichlorophenol

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

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