Isolation and Characterization of Phosphate-Solubilizing Bacteria from Rhizosphere Soil

Sadiq, Hafiza Madeha; Jahangir, Ghulam Zahara; Nasir, Idrees Ahmad; Iqtidar, Mehwish; Iqbal, Muhammad Asif

doi:10.5504/bbeq.2013.0091

Cited by 36 publications

(24 citation statements)

References 22 publications

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“…are recognized as phosphate solubilizing bacteria from the rhizosphere of crop plants as reported from Korea (Chung et al 2005). The different strains of phosphate solubilizing bacteria Proteus vulgaris, K. pneumoniae, E. aerogens, Burkholderia cepaciae, Citrobacter freundii, A. lwoffi and Pseudomonas fluorescens isolated from the rhizosphere of different plants has been reported as efficient for phosphate solubilization by forming halozones on agar plates of Pikovskaya growth medium (PVK) by solubilizing tricalcium phosphate of the medium (Sadiq et al 2013). The plant growth-promoting P-solubilizing bacteria such as Bacillus circulans has been investigated as the potential strain from the rhizospheric region of apple plant, Himachal Pradesh, India (Mehta et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Kshetri

Pandey

Sharma

2018

Journal of Plant Interactions

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This work describes integrated nutrient management for cultivation of Allium hookeri by using phosphate solubilizing bacteria (PSB) applied in rhizosphere, along with tricalcium phosphate (TCP). Arthrobacter luteolus S4C7, Enterobacter asburiae S5C7, Klebsiella pneumoniae S4C9, S4C10 and S6C1, and K. quasipneumoniae S6C2, were isolated from rhizosphere of Allium hookeri Thwaites, and were found to release substantial amount of soluble phosphate (124.8-266.4 μg/mL) from TCP in vitro conditions. These isolates were experimented for plant growth promoting attributes, including IAA, siderophore, and nitrogen-fixation. Treatment with PSB resulted in enhanced growth of A. hookeri Th., which was even better with TCP amendment with PSB. K.quasipneumoniae S6C2 resulted in 39.1% and 533.3% increase (p ≤ 0.05) of root length and weight respectively. The treatment with these isolates, in TCP amended soil also resulted in 200-250% increase in available P in soil, which was maximum for K. quasipneumoniae (1.866 mg/g). ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Kshetri

Pandey

Sharma

2018

Journal of Plant Interactions

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“…In addition, such inoculants have been shown to influence root growth and the expression of Pi transporter genes [24][25][26]. Some recent studies have focused on the isolation, characterization and evaluation of PSB and PMB with potential to promote plant growth under sub-optimal conditions [27][28][29][30][31]. In this context, the aim of the present work was to evaluate the effect of plant inoculation with two autochthonous Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation

et al. 2020

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Certain phosphorous solubilizing (PSB) and phosphorous mineralizing (PMB) bacteria may improve plant growth by improving nutrient availability. The aim of this work was to evaluate the effect of inoculation with two Bacillus spp. strains, 12A and 25A, on wheat seedlings growth. To this aim, a durum and a bread wheat genotype were grown under controlled conditions in a low P compost medium to evaluate: (i) the effect of the bacterial isolates on plant growth and root system architecture; (ii) the expression of two key genes indicative of the P-starvation response and phosphate (Pi) uptake, TaIPS1 and TaPHT1.6-B1. The results showed that 12A Bacillus sp. significantly increased root length, surface area and biomass. Furthermore, an enhanced shoot dry weight and P content were observed. This might be explained by the capacity of strain 12A to produce indole-3-acetic acid (IAA) in addition to P mineralizing and P solubilizing capability. No effect on plant growth was observed for 25A strain. The semi-quantitative gene expression analysis showed an overall lower expression of TaIPS1 in the inoculated plants and highest expression of TaPHT1.6-B1 in 12A inoculated plants. This suggests that Pi-responsive genes might be useful molecular indicators for the effectiveness of PSB and PMB.

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“…As Bashan et al ( 23 ) proposed, our results highlight that adequate selection of phosphate-solubilizing strains requires the assessment of this ability in both solid and liquid media. Phosphorus solubilization in the NBRIP broth was also found to depend on the nitrogen and carbon sources present ( 25 – 27 ). As observed for B02, L3, and Sp20, Kshetri et al ( 28 ) obtained higher solubilization in rhizobia when diammonium sulfate was used as a nitrogen source.…”

Section: Discussionmentioning

confidence: 99%

A Framework for the Selection of Plant Growth-Promoting Rhizobacteria Based on Bacterial Competence Mechanisms

Amaya-Gómez

Porcel

Mesa-Garriga

et al. 2020

Appl Environ Microbiol

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ABSTRACT The use of plant growth-promoting rhizobacteria (PGPR) is increasingly meaningful for the development of more environmentally friendly agricultural practices. However, often the PGPR strains selected in the laboratory fail to confer the expected beneficial effects when evaluated in plant experiments. Insufficient rhizosphere colonization is pointed out as one of the causes. With the aim of minimizing this inconsistency, we propose that besides studying plant growth promotion traits (PGP), the screening strategy should include evaluation of the microbial phenotypes required for colonization and persistence. As a model, we carried out this strategy in three Rhizobium sp. strains that showed phosphorus solubilization ability and production of siderophores. All strains displayed colonization phenotypes like surface spreading, resistance to hydrogen peroxide, and formed biofilms. Regarding their ability to persist, biofilm formation was observed to be influenced by pH and the phosphorus nutrient provided in the growth media. Differences in the competence of the strains to use several carbon substrates were also detected. As part of our framework, we compared the phenotypic characteristics of the strains in a quantitative manner. The data analysis was integrated using a multicriteria decision analysis (MCDA). All our results were scored, weighted, and grouped as relevant for PGP, colonization, or persistence. MCDA demonstrated that, when the phenotypes related to PGP and colonization are weighted over those for persistence, strain B02 performs better than the other two Rhizobium sp. strains. The use of our framework could assist the selection of more competent strains to be tested in greenhouse and field trials. IMPORTANCE Numerous plant growth-promoting rhizobacteria (PGPR) have been inoculated into the soil with the aim of improving the supply of nutrients to crop plants and decreasing the requirement of chemical fertilizers. However, sometimes these microbes fail to competitively colonize the plant roots and rhizosphere. Hence, the plant growth promotion effect is not observed. Here, we describe a new screening strategy aiming at the selection of more competent PGPR. We evaluated bacterial phenotypes related to plant growth promotion, colonization, and persistence. Our results demonstrated that despite the fact that our Rhizobium sp. strains successfully solubilized phosphorus and produced siderophores, their abilities to spread over surfaces, resist hydrogen peroxide, and form biofilms varied. Additionally, a multicriteria decision analysis was used to analyze the data that originated from bacterial physiological characterizations. This analysis allowed us to innovatively evaluate each strain as a whole and compare the performances of the strains under hypothetical scenarios of bacterial-trait requirements.

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Isolation and Characterization of Phosphate-Solubilizing Bacteria from Rhizosphere Soil

Cited by 36 publications

References 22 publications

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation

A Framework for the Selection of Plant Growth-Promoting Rhizobacteria Based on Bacterial Competence Mechanisms

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