Isolation and characterization of pigment-protein particles from the light-harvesting complex of Phaeodactylum tricornutum

Gugliemelli, L. A.

doi:10.1016/0005-2728(84)90215-9

Cited by 22 publications

(8 citation statements)

References 17 publications

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“…Amino acid sequence analysis for both subunits and fragments thereof failed due to N-terminal blockage of the poly- Alberte (1984). Gugliemelli (1984) and Owens and Wold (1986). The inability to estimate Mr values of native complexes due to the easy disintegration by detergents is a well known problem for chromophyte LHCs.…”

Section: Discussionmentioning

confidence: 98%

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The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

Rhiel¹,

Marquardt²,

Eppard³

et al. 1997

Botanica Acta

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Section: Discussionmentioning

confidence: 98%

“…for several brown algae (Passaquet et al, 1991) and for F? tricornutum (Gugliemelli, 1984). These Mr values ranged from 50000 up to 240000 with the great variability being most probably caused by the different isolation procedures.…”

Section: Discussionmentioning

confidence: 99%

The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

Rhiel¹,

Marquardt²,

Eppard³

et al. 1997

Botanica Acta

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“…Biochemical work on FCPs dates back to the 80s, when mainly sucrose density centrifugation was used to separate photosystems from FCPs as a whole (Alberte et al 1981 ;Brown 1988 ;Caron and Brown 1987 ;Fawley et al 1986 ;Alberte 1984 , 1986 ;Gugliemelli 1984 ;Owens 1986Owens , 1988Owens and Wold 1986 ). Most FCPs can be isolated as 'free' FCPs, i.e.…”

Section: B Supramolecular Organization Of Fucoxanthin-chlorophyll Prmentioning

confidence: 99%

Structure and Functional Heterogeneity of Fucoxanthin-Chlorophyll Proteins in Diatoms

Gundermann

Büchel

2014

The Structural Basis of Biological Energy Generation

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SummaryFucoxanthin-chlorophyll proteins (FCPs) of diatoms are divided into three groups, the main light harvesting antennas Lhcf, the photosystem I-specifi c Lhcr, and Lhcx involved in photoprotection. All are closely related to higher plant light harvesting complexes (LHCs) when comparing sequences, albeit smaller and more hydrophobic. However, pigmentation differs from higher plant LHCs with around eight chlorophyll a , two chlorophyll c and six fucoxanthin per monomer. Fucoxanthin, with a carbonyl moiety conjugated to the polyene backbone, undergoes extreme bathochromic shifts upon protein binding, dividing the different fucoxanthins into more red, green and blue absorbing ones. Excitation energy transfer is extremely effi cient, either directly from chlorophyll c to chlorophyll a or from fucoxanthin to chlorophyll a involving the S 1 /ICT state of fucoxanthin. Most Lhcf assemble into trimers, whereby only in centric diatoms Lhcx was found in trimers as well, and specifi c oligomeric FCP complexes are present. Whereas the arrangement of FCPs around the photosystems is largely unknown, spectroscopic measurements together with homology considerations allow for a fi rst rough model of the pigment arrangement in trimeric and oligomeric FCP complexes. Blue fucoxanthin is bound analogously to lutein in LHCII, surrounded by the same four chlorophyll a , since binding sites are conserved. Additionally, chlorophyll a can be found in a604, a614, b605 and a611, although binding of the latter has to be different due to the lack of long wavelength absorption in FCPs. Chlorophyll c is most probably bound in b609 and a613. The red fucoxanthin cluster around helix 2, which has less sequence homology to LHCII. The green fucoxanthins are most probably located around the violaxanthin and b601 binding sites of LHCII, whereby the former is probably a mixed site for fucoxanthins and diadinoxanthin/diatoxanthin.

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“…Almost all investigations of diatom pigment-protein complexes have been carried out with one species, Phaeodactylum tricornutum. Chl-protein complexes which have either reaction centers for PSI (1,27)orantennapigments (1, [14][15][16]18,27) have been isolated from this species. My goal was to determine if other diatoms have similar complexes.…”

Section: Kej Index Words: Chlorophyll-carotenoid-protein Complexes; Dmentioning

confidence: 99%

PHOTOSYNTHETIC PIGMENT ORGANIZATION IN DIATOMS (BACILLARIOPHYCEAE)¹

Brown

1988

Journal of Phycology

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The isolation and characterization of six pigment‐protein complexes from five diatom species (Phaeodactylum tricornutum Bohlin, Chaetoceros gracilis Schutt, Nitzschia sp. Mono Lake, Nitzschia laevis Hust. and Thalassiosira pseudonana (Hust.) Hasle and Heimdal) was accomplished by membrane dissociation with digitonin followed by gel electrophoresis. Six analogous complexes obtained from all species were correlated in spectral characteristics and relative mass with complexes from higher plants obtained by the same procedure. The largest of these complexes, comprising about 15% of the total Chl a, contained reaction centers of Photosystem I (PSI) and antenna pigments (LHC1).Some PSI complexes also separated from LHC1 in the gel. For the first time in diatoms, a Photosystem II complex was isolated and identified from its position in the gels, absorption and fluorescence spectra, lack of P700, and enrichment carotene. Three antenna pigment‐protein complexes in addition to LHC1 occurred in varying proportion under different experimental conditions but in sum, they accounted for 70% of the total Chl a. All three bands were highly enriched in Chl c and fucoxanthin, although the ratio of Chl c/ xanthophyll decreased between the slowest migrating LHC2 and fastest moving LHC4 LHC3 contained the highest proportio of pigment‐protein and was composed primarily of polypeptides of about 18,000 D. Essentially all α‐ and β‐carotene was bound to the reaction center complexes. The Nitzschia from Mono Lake differed from the other species in that PSI complexes could not be readily dissociated from its membrane by digitonin treatment, a characteristic which may reflect a different chloroplast membrane structure in this alga.

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Isolation and characterization of pigment-protein particles from the light-harvesting complex of Phaeodactylum tricornutum

Cited by 22 publications

References 17 publications

The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

Structure and Functional Heterogeneity of Fucoxanthin-Chlorophyll Proteins in Diatoms

PHOTOSYNTHETIC PIGMENT ORGANIZATION IN DIATOMS (BACILLARIOPHYCEAE)¹

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Isolation and characterization of pigment-protein particles from the light-harvesting complex of Phaeodactylum tricornutum

Cited by 22 publications

References 17 publications

The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

The Light Harvesting System of the Diatom Cyclotella cryptica. Isolation and Characterization of the Main Light Harvesting Complex and Evidence for the Existence of Minor Pigment Proteins*

Structure and Functional Heterogeneity of Fucoxanthin-Chlorophyll Proteins in Diatoms

PHOTOSYNTHETIC PIGMENT ORGANIZATION IN DIATOMS (BACILLARIOPHYCEAE)1

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PHOTOSYNTHETIC PIGMENT ORGANIZATION IN DIATOMS (BACILLARIOPHYCEAE)¹