Isolation and characterization of plasma lipoproteins of common marmoset monkey. Comparison of effects of control and atherogenic diets.

Crook, David; Weisgraber, Karl H.; Boyles, J K; Mahley, Robert W.

doi:10.1161/01.atv.10.4.633

Cited by 19 publications

(18 citation statements)

References 36 publications

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“…Indeed, the invariant diameter of Lp(a) across the density interval from 1.048 to 1.076 g/ml distinguished it from LDL, which presented as one or more bands per subfraction and which decreased progressively in size (from a major component of 29 nm diameter at d=1.019 g/ml to one of 25.2 nm at d=1.060 g/ml) with an increase in density. Considerable overlap in the density distributions of LDL and HDL occurred in fraction 10 of d=1.055-1.065 g/ml, as reported earlier 15 and recently confirmed 18 ; in addition, Lp(a) was a component of this subfraction (Figure 2). …”

Section: Figure 2 Photograph Showing Results Of Nondenaturing Polyacsupporting

confidence: 76%

“…1516 More recently, the susceptibility of the marmoset to dietinduced atherosclerosis, as originally described by Dreizen et al, 17 has been confirmed 18 and in addition has been extended to demonstrate the occurrence of both foam cell lesions and proliferative intimal plaques in monkeys hyperresponsive to diets supplemented with cholesterol and saturated fat. 18 To date, both biochemical and immunologic studies have failed to reveal the presence of circulating Lp(a) in the plasmas of New World monkeys, including the common marmoset. 31518 As plasma Lp(a) levels may vary over a wide range both in humans and in nonhuman primates,…”

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confidence: 85%

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Lipoprotein(a) and apolipoprotein(a) in a New World monkey, the common marmoset (Callithrix jacchus). Association of variable plasma lipoprotein(a) levels with a single apolipoprotein(a) isoform.

Guo

Michel

Blouquit

et al. 1991

Arterioscler Thromb

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In an earlier report (Chapman et al, Biochemistry 1979;18:5096-5108), we suggested that the common marmoset may represent an important model for the study of human plasma lipoprotein metabolism. We now extend the interest of this monkey model to the study of lipoprotein(a) (Lp[a]) and apolipoprotein(a) (apo[a]). Density gradient ultracentrifugal fractionation of marmoset plasma revealed a bimodal distribution of Lp(a), with one peak of concentration occurring in association with very low density lipoproteins (VLDLs) and a second in the density range 1.040-1.080 g/ml. The dense Lp(a) subspecies displayed physicochemical properties (chemical composition, particle size, and electrophoretic mobility) that closely resembled those of its counterpart in humans and baboons but that were distinct from those of low density lipoprotein (LDL). Furthermore, the particle size of marmoset Lp(a) was invariant (31 nm) over the density interval 1.040-1.080 g/ml, whereas that of LDL decreased progressively with an increase in density (-26-25.2 nm). Use of polyclonal and monoclonal antibodies to human apo(a) and of a polyclonal antibody to marmoset Lp(a) allowed immunologic identification of a single apo(a) isoform in the marmoset whose size was similar to that of apo B-100; apo(a) and apo B-100 were associated in Lp(a) particles by a disulfide linkage. The total protein mass of apo-Lp(a) was estimated to be 800,000 or more by electrophoresis in sodium dodecyl sulfate-polyacrylamide-agarose gels. The amino acid compositions of marmoset and human apo(a) resembled each other but were distinct from those of the corresponding forms of apo B-100. Immunologic evidence is provided for a high degree of cross reactivity between apo(a) in marmosets, baboons, and humans, supporting the idea of the existence of a marked degree of structural homology between these proteins. In addition, electroimmunoblotting of marmoset apo(a) and marmoset plasminogen showed that these proteins shared certain epitopes in common, suggesting that marmoset apo (

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Section: Figure 2 Photograph Showing Results Of Nondenaturing Polyacsupporting

confidence: 76%

mentioning

confidence: 85%

Lipoprotein(a) and apolipoprotein(a) in a New World monkey, the common marmoset (Callithrix jacchus). Association of variable plasma lipoprotein(a) levels with a single apolipoprotein(a) isoform.

Guo

Michel

Blouquit

et al. 1991

Arterioscler Thromb

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“…The common marmoset, a New World monkey, has become increasingly attractive as an experimental animal model for studying the pharmacology [11], immunology [40], virology [43], and toxicology [26,33] involved in the the vertex, and the reference electrode (-), at the dorsal base of the stimulated ear. The ground electrode was subcutaneously placed at the dorsal part of the neck.…”

Section: Subjectsmentioning

confidence: 99%

Effects of Click Intensity and Frequency on the Brain-Stem Auditory Evoked Potentials in the Common Marmoset (Callithrix jacchus).

Harada

Tokuriki

1997

J. Vet. Med. Sci.

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ABSTRACT. Brain-stem auditory evoked potentials (BAEPs) were recorded in 20 common marmosets (Callithrix jacchus) to investigate the effects of click frequency up to 99 kHz, in consideration of the higher hearing range of the marmoset, and intensity on wave forms and peak latencies. According to the results of BAEP recordings at frequencies of 4, 32, and 99 kHz, the number of components recorded was affected by the stimulus intensity and the clicks at an intensity of 80 dB peak equivalent sound pressure level (pe SPL) had the maximum number of clear components. Therefore, it was indicated that click stimulations at an intensity of 80 dB pe SPL over a broad range of frequencies appears to be useful for recording the maximum number of components in marmosets and may increase the information obtainable from BAEPs. BAEP latencies were prolonged as the stimulus intensity decreased from 100 to 50 dB pe SPL. The effects of stimulus frequency on the wave latencies and amplitudes in response to 80 dB pe SPL at frequencies between 0.5 and 99 kHz revealed various changes: the amplitude of wave I increased at 16 and 32 kHz, but that of waves III and V increased at 4-8 and 64-99 kHz. These increases in amplitudes of the waves may correlate with higher synchronous activity of the peripheral or central auditory pathways. -KEY WORDS: brain-stem auditory evoked potential (BAEP), click stimulation, marmoset, stimulus frequency, stimulus intensity.

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“…In either the experimental animal or man, the steady-state concentration of cholesterol carried in low density lipoproteins is usually low when the dietary intake of cholesterol and triacylglycerol is minimal (1)(2)(3)(4)(5) (unpublished observations in cynomolgus monkey). In this situation where little lipid is reaching the liver in the chylomicron remnant, the rate at which LDL cholesterol is being produced and enters the plasma space is low, relative to the rate at which LDL can be removed from the plasma by receptor-dependent transport.…”

Section: Introductionmentioning

confidence: 99%

Regulatory effects of the saturated fatty acids 6:0 through 18:0 on hepatic low density lipoprotein receptor activity in the hamster.

Woollett¹,

Spady²

1992

J. Clin. Invest.

121

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The plasma concentration of cholesterol carried in low density lipoproteins is principally determined by the level of LDL receptor activity (P) and the LDL-cholesterol production rate (Jt) found in animals or man. This study delineates which saturated fatty acids alter JU and Jt and so increase the plasma LDL-cholesterol level. J' and Jt were measured in vivo in hamsters fed a constant level of added dietary cholesterol (0.12%) and triacylglycerol (10%), where the triacylglycerol contained only a single saturated fatty acid varying in chain length from 6 to 18 carbon atoms. After feeding for 30 d, the 12:0, 14:0, 16:0, and 18:0 fatty acids, but not the 6:0, 8:0, and 10:0 compounds, became significantly enriched in the liver total lipid fraction of the respective groups fed these fatty acids. However, only the 12:0, 14:0, and 16:0 fatty acids, but not the 6:0, 8:0, 10:0, and 18:0 compounds, suppressed J, increased Jt, and essentially doubled plasma LDL-cholesterol concentrations. Neither the 16:0 nor 18:0 compound altered rates of cholesterol synthesis in the extrahepatic organs, and both lowered the hepatic total cholesterol pool. Thus, the different effects of the 16:0 and 18:0 fatty acids could not be attributed to a difference in cholesterol delivery to the liver. Since these changes in LDL kinetics took place without an apparent alteration in external sterol balance, the regulatory effects of the 12:0, 14:0, and 16:0 fatty acids presumably are mediated through some change in a putative intrahepatic regulatory pool of sterol in the liver. (J.

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Isolation and characterization of plasma lipoproteins of common marmoset monkey. Comparison of effects of control and atherogenic diets.

Cited by 19 publications

References 36 publications

Lipoprotein(a) and apolipoprotein(a) in a New World monkey, the common marmoset (Callithrix jacchus). Association of variable plasma lipoprotein(a) levels with a single apolipoprotein(a) isoform.

Lipoprotein(a) and apolipoprotein(a) in a New World monkey, the common marmoset (Callithrix jacchus). Association of variable plasma lipoprotein(a) levels with a single apolipoprotein(a) isoform.

Effects of Click Intensity and Frequency on the Brain-Stem Auditory Evoked Potentials in the Common Marmoset (Callithrix jacchus).

Regulatory effects of the saturated fatty acids 6:0 through 18:0 on hepatic low density lipoprotein receptor activity in the hamster.

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