Isolation and characterization of plasma membrane Na+ /H+ antiporter (SOS1) gene during salinity stress in kallar grass (Leptochloa fusca)

Abstract

Background: Leptochloa fusca is a halophyte plant which is highly tolerant to saline and sodic soils and water. Moreover, L. fusca is an attractive model plant to study the mechanism of salt tolerance mainly due to its characteristics as a typical euhalophyte, having both accumulating and excreting salt properties. Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane Na + /H + antiporter that plays an important role in imparting salt stress tolerance of plants. Material and Methods: Using conserved sequences of SOS1, the coding sequence of plasma membrane Na + /H + antiporter (SOS1) in kallar grass was partially isolated and its expression profile during salinity stress was investigated. Results: The aa (amino acid) sequence of the isolated region of LfSOS1 possesses the maximum identity up to 96% of its orthologue in Distichlis spicata. The results of semi-quantitative RT-PCR revealed that salinization was affected SOS1 transcript level positively. The expression of LfSOS1 in leaves of kallar grass progressively increased under all salinity levels compared to control. Conclusions:The results suggest that LfSOS1 may play an essential role in the salt tolerance of L. fusca and may be useful for improving salt tolerance in other crop species.

Tasos1 and Tatm20 Genes Expression and Nutrient Uptake in Wheat Seedlings May Be Altered via Excess Cadmium Exposure and Inoculation With Azospirillum Brasilense Sp7 Under Saline Condition

“…Ramezani et al (2013) and Kim et al, (2008) The upregulation of salt overly sensitive 1 (TaSOS1) and TaTM20 under saline condition (Ramezani et al, 2013) and TaTM20 upregulation under Cd polluted conditions (Kim et al, 2008) also showed that yeast transgenic containing TaSOS1 gene has higher salt tolerance as compared to non-transgenic ones. In addition, Taherinia et al (2015) revealed that salinization was affected SOS1 transcript level positively in kallar grass (Leptochloa fusca L.) a halophyte plant which is highly tolerant to saline and sodic soil and water. Accordingly, it can be proposed that Azospirillum species may contribute to the upregulation of TaSOS1 and TaTM20 gene expression in wheat plants under saline and Cd polluted condition and improve salinity and Cd tolerance.…”