Isolation and characterization of plasmid DNA fromRuminococcus

Asmundson, R. V.; Kelly, William J.

doi:10.1007/bf01588178

Cited by 30 publications

(20 citation statements)

References 19 publications

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“…Small cryptic plasmids are abundant in rumen bacteria [8,19,20]. In this class, the plasmids, carrying both the replication and mobilization proteins, have been detected in Butyrivibrio ¢brisolvens [21,22], Prevotella ruminicola [8], and Ruminococcus albus [23].…”

Section: Discussionmentioning

confidence: 99%

A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

Ogata¹

1999

FEMS Microbiology Letters

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The complete nucleotide sequence of a small cryptic plasmid designated pRAO1, from the Gram-negative ruminal bacterium Ruminobacter amylophilus NIAH-3, was determined. The plasmid is a circular DNA molecule, 2140 bp in size, with a GC content of 40%. Computer-assisted analysis identified three open reading frames (ORFs), one of which, ORF3 (347 amino acids), displayed a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmid recombination of plasmids from Gram-positive bacteria. We proved the mobilization properties of pRAO1 in the Escherichia coli system using the coresident IncW broad-host-range conjugative plasmid R388. These data demonstrated, for the first time, the mobilization properties of small cryptic plasmids from Gram-negative inhabitants of the rumen. ß 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

A small cryptic plasmid from Ruminobacter amylophilus NIAH-3 possesses functional mobilization properties

Ogata¹

1999

FEMS Microbiology Letters

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“…R. albus F-40 used for isolation of chromosomal DNA was cultivated as described previously, 11) at 37 C overnight in 100 ml of the modified GS medium (pH 6.8) containing 0.5% cellobiose as a carbon source.…”

Section: Methodsmentioning

confidence: 99%

Cloning of theRuminococcus albus cel5Dandcel9AGenes Encoding Dockerin Module-containing Endoglucanases and Expression ofcel5DinEscherichia coli

Taguchi¹,

Hagiwara²,

Genma³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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“…To achieve stable introduction of heterologous DNA into rumen bacteria requires the development of a stable vector system. It should be noted that cryptic plasmids have been described in R. albus, R. jlavefaciens (Asmundson & Kelly, 1987), B. jibrisolvens (Teather, 1982;Mann et al 1986) and S. ruminantium (Orpin et al 1986b;Martin & Dean, 1989). Plasmids from the latter organism have been cloned into pUC-based vectors, and their mode of replication is being examined to assess their utility as cloning vectors (J. Murray and G. P. Hazlewood, unpublished results (Luchansky et al 1988), and it is likely that in the near future transformation systems for rumen bacteria, based on electroporation, will be developed.…”

Section: Genetic Manipulation Of Organismsmentioning

confidence: 99%