Isolation and characterization of polymorphic microsatellites in <i>Labeo rohita</i> and their cross‐species amplification in related species

Das, Paramananda; Barat, Ashoktaru; Meher, Prem Kumar; Ray, Phillip; Majumdar, D.

doi:10.1111/j.1471-8286.2005.00905.x

Cited by 38 publications

(34 citation statements)

References 3 publications

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“…gov). For cross-species amplification of microsatellite loci, a total of 40 microsatellite primers from Catla catla [24]; Cyprinus carpio [25,26]; Barbodes gonionotus [27]; Campostoma anamalum [28]; Labeo rohita [29] and Pimephales promelas [30] were used ( Table 1). The crossspecies amplification trials were done with eight specimens of G. curmuca and of the 40 primer pairs tested, 34 (85 %) provided successful amplification of homologous loci in G. curmuca.…”

Section: Pcr Amplification and Selection Of Microsatellite Locimentioning

confidence: 99%

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

et al. 2014

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Gonoproktopterus curmuca is an endangered red tailed barb found in Southern part of Western Ghat, India. As a part of stock-specific, propagation assisted rehabilitation and management program, polymorphic microsatellites markers were used to study the genetic diversity and population structure of this species from the three River systems of Southern Western Ghats, such as Periyar River, the Chalakkudy River, and the Chaliyar River. From selected eight polymorphic microsatellite markers, the number of alleles per locus ranged from 2 to 8, and the average number of alleles among 3 populations ranged from 5.0 to 5.75. The mean observed (H ob ) and expected (H ex ) heterozygosity ranged from 0.5148 to 0.5360 and from 0.5996 to 0.6067, respectively. Significant deviations from Hardy-Weinberg Equilibrium expectation were found at majority of the loci (except Gcur MFW72 and Gcur MFW19) and in all three populations in which heterozygote deficits were apparent. The analysis of molecular variance indicates that the percent of variance among populations and within populations were 6.73 and 93.27, respectively. The pairwise F ST values between populations indicate that there were significant deviations in genetic differentiations for the red-tailed barb populations from these three Rivers of the Western Ghats, India. The microsatellites methods reported a low degree of gene diversity and lack of genetic heterogeneity in the population of G. curmuca, which strongly emphasize the need of fishery management, conservation and rehabilitation of G. curmuca.

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Section: Pcr Amplification and Selection Of Microsatellite Locimentioning

confidence: 99%

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

et al. 2014

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“…Again in these same four loci the average observed heterozygosity and expected heterozygosity were 0.746 and 0.621 (Das et al, 2005) and 0.413 and 0.493 (Alam et al, 2009) in the Indian major carp populations. We observed that average observed heterozygosity and expected heterozygosity was lower than that of obtained in the study by Das et al (2005) but higher than that of obtained by Alam et al (2009).…”

Section: Discussionmentioning

confidence: 90%

“…Khan et al (2006) using allozyme marker reported 27 % polymorphic loci in the three river population of L. rohita. Das et al (2005) Nei's (1972) developed microsatellite markers obtained all the studied loci polymorphic in an Indian farmed population of L. rohita. Using four loci they obtained a total of 20 alleles (5 alleles in average) and Alam et al (2009) obtained only 14 alleles, while we obtained 20 alleles by using three loci.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, microsatellites are codominant markers, highly polymorphic, easily typed, and Mendelian inherited which facilitate for population structure study, pedigree analysis as well as detecting differences among closely related species (AbdulMuneer, 2014). Microsatellite markers have been found to be effective in detecting high levels of polymorphism and rare alleles as well as population structure analysis of three Indian major caprs (Naish & Skibinski, 1998;Das, Baret, Meher, Ray, & Majumdar, 2005). These markers are now widely used for the determination of genetic variation in wild and cultured fish populations (Was & Wenne, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Shah¹,

Sutapa²,

Islam³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Labeo rohita is an important commercial aquaculture species in Bangladesh, but it needs improved growth performance to meet the increasing demand for fish. Genetic improvement through inter-strain crossing, generating heterosis, is quicker than a selection strategy. Therefore, crosses were made between two strains of rohu: Padma River strain and a hatchery strain. Growth performance analyis showed the highest growth rate in the Padma × Padma cross followed by one of the reciprocal crosses Hatchery♂ × Padma♀ (hybrid). The lowest growth rate was observed in Hatchery × Hatchery cross. DNA variability study using three microsatellite markers showed polymorphism (P95) in these crosses. Variations were observed in number and frequencies of alleles and mean heterozygosity in different loci; the mean number of alleles varied from 4.667±0.882, 3.333±1.333 and 2.667±1.202 in Padma, hybrid and Hatchery strains respectively. Relatively high levels of gene flow and low Strain differentiation (FST ) values were found between the Padma and hybrid strains. The UPGMA dendrogram based on genetic distance placed the Padma population in one cluster and the hybrid and Hatchery populations in another. The results revealed that there was relatively low level of genetic variability in the riverine and hatchery reared populations of L. rohita in Bangladesh.

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“…But earlier, using same loci, Das et al, (2005) found the observed heterozygosity to be higher than expected heterozygosity in the Indian population of L. rohita which is in accordance to the present findings and these results can be due to the fact that several evolutionary forces like random genetic drift, migration, mutation and their mutual interactions act on the wild populations and influence the pattern of genetic differentiation (Avise, 1994). Charlesworth and Charlesworth, (2016) also suggested that the polymorphism present in natural populations differs markedly in different species and also within the species.…”

Section: Microsatellite Based Genetic Variations Between the Populationsmentioning

confidence: 98%

Genetic Diversity Analysis of Labeo rohita (Hamilton, 1822) Collected from Two Zones of River Ganga Using Microsatellite DNA Markers

Joshi¹,

Lohni²,

Ram³

2017

Int.J.Curr.Microbiol.App.Sci

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Isolation and characterization of polymorphic microsatellites in Labeo rohita and their cross‐species amplification in related species

Cited by 38 publications

References 3 publications

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

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Genetic Diversity Analysis of Labeo rohita (Hamilton, 1822) Collected from Two Zones of River Ganga Using Microsatellite DNA Markers

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