Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus)

Padilla, J. A.; Parejo, Juan Carlos; Salazar, J.; Martínez-Tranćon, M.; Rabasco, A.; Sansinforiano, E.; Quesada, Alberto

doi:10.1007/s10592-008-9711-1

Cited by 16 publications

(12 citation statements)

References 5 publications

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“…LD was found only between SNMS28 and SNMS32 (P \ 0.05). The present expected heterozygosity was lower than that obtained from microsatellite analyses of Northern Goshawks (Topinka and May 2004;Takaki et al 2009) and Falco species (Padilla et al 2009). This lower genetic diversity may result from the very narrow range from which the present samples were collected.…”

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confidence: 80%

“…The Philippine Hawk-Eagle (S. philippensis) is also threatened (BirdLife International 2009). Microsatellite primers are sometimes applicable to closely related species (Padilla et al 2009). If the present primer set or sequence information is applicable to these threatened species, this analysis may provide fundamental information for the conservation of threatened species in the genus Spizaetus.…”

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confidence: 99%

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Isolation and characterization of eleven microsatellite loci in an endangered species, Mountain Hawk-Eagle (Spizaetus nipalensis)

Hirai

Yamazaki²

2010

Conservation Genet Resour

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confidence: 80%

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confidence: 99%

Isolation and characterization of eleven microsatellite loci in an endangered species, Mountain Hawk-Eagle (Spizaetus nipalensis)

Hirai

Yamazaki²

2010

Conservation Genet Resour

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“…In recent years, a number of microsatellite techniques have been developed for several raptor species Martínez-Cruz 2002;Busch et al 2005;Ortego et al 2007;Tingay et al 2007;Padilla et al 2008) and a multiplex PCR assay has also been established for the whitetailed sea eagle (Hailer et al 2005). Multiplex PCR methods reduce several analyses into one (Edwards & Gibbs 1994), thereby simplifying analysis.…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR amplification of 13 microsatellite loci for Aquila chrysaetos in forensic applications

et al. 2010

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The golden eagle (Aquila chrysaetos) is an endangered raptor, which is threatened mainly by illegal egg and nestling robbery. Here we describe a fluorescently labeled, multiplex PCR method using 13 microsatellite markers, which provides a powerful tool for the individual identification and parentage testing of the Golden eagle. This test should be applicable to both forensic analysis and population studies. Fifteen polymorphic loci from A. chrysaetos were crossamplified. Subsequent PCR condition optimization led to the successful co-amplification of 13 different loci in a single PCR reaction. Fifty samples from wild-living individuals and 89 samples from captive-bred individuals were examined. The results indicated that both populations have similar levels of moderate inbreeding, unsurprising in a small population. This probability of excluding a random individual in parentage analysis was 0.9912 for the wild population and 0.9932 in the captive-bred one in the case that both the individual and its mother were examined together. The probability of identity was estimated to be 3 × 10 −8 for the wild and 4 × 10 −8 for the captive-bred populations. Given the size of the Slovak golden eagle population, this test should therefore be sufficient to reliably identify individual raptors and assess parentage in both conservation studies and forensic analysis.

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“…We used a ZR Genomic DNA II Kit (Zymo Research, Irvine, CA, USA) to extract and purify genomic DNA from the blood samples. The DNA samples were genotyped across 12 polymorphic microsatellite markers designed for F. naumanni species: FND1_2, FND1_4, FND1_5, FND1_6, FND1_7, FND1_8, FND2_1, FND2_2, FND2_3, FND2_4, FND2_5, and FND2_6 (Padilla et al, 2009;Ortego et al, 2008a). We used GIMLET 1.33 (Valiere, 2002) to perform two independent polymerase chain reaction (PCR) replicates to check the absence of allelic dropout or false alleles.…”

Section: Blood Sample Collection and Genetic Analysesmentioning

confidence: 99%

Do not disturb the family: roles of colony size and human disturbance in the genetic structure of lesser kestrel

et al. 2014

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Dispersal and philopatry are fundamental processes influencing the genetic structure and persistence of populations, and might be affected by isolation and habitat perturbation. Habitat degradation induced by human activities could have detrimental consequences on the genetic structure of populations. Therefore, it is crucial to understand the role of human impact in promoting or disrupting the genetic structure. Here, we conducted a genetic analysis using 12 polymorphic microsatellite markers of 70 lesser kestrels Falco naumanni from 10 breeding colonies of two subpopulations in Sicily (southern Italy). Genetic differentiation between the two subpopulations was negligible, and linear distances played no role in the level of genetic relatedness recorded in the two sites. Linear distances between nests also resulted in no effects on the relatedness recorded within and between colonies in the largest subpopulation. Clusters of more-versus less-related individuals resulted when the two-dimensional positions of colonies (i.e., latitude and longitude) were tested as predictors of genetic proximity instead of linear distances. Specifically, analyses of colony features showed colony size and human disturbance as factors negatively affecting the relatedness among chicks from different nests. Regardless of colony size, less-related individuals were born in colonies located in the core of the agricultural plain, where we quantified a higher level of human disturbance. In contrast, more related individuals were in colonies located in the marginal, less disturbed, agricultural area. Given the high philopatry of this species, our results are consistent with disruption of colony fidelity related to intensification of agricultural practices. We discuss the possible implications of long-term effects of genetic variability in small and disturbed colonies on fitness and population viability.

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Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus)

Cited by 16 publications

References 5 publications

Isolation and characterization of eleven microsatellite loci in an endangered species, Mountain Hawk-Eagle (Spizaetus nipalensis)

Isolation and characterization of eleven microsatellite loci in an endangered species, Mountain Hawk-Eagle (Spizaetus nipalensis)

Multiplex PCR amplification of 13 microsatellite loci for Aquila chrysaetos in forensic applications

Do not disturb the family: roles of colony size and human disturbance in the genetic structure of lesser kestrel

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