Isolation and characterization of polymorphic microsatellite loci from an EST library of turbot (Scophthalmus maximus) and cross‐species amplification

Abstract

In the present study, we report 12 polymorphic microsatellite loci developed from a cDNA library from the turbot, Scophthalmus maximus. Observed and expected heterozygosities varied from 0.20 to 1.00 and from 0.18 to 0.78, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Cross‐species amplifications of these microsatellites in five additional fish species revealed between five and 11 positive amplifications and between zero and four polymorphic loci per species.

Genetic analysis of gynogenetic and common populations of Verasper moseri using SSR markers

“…However, the low genetic variability usually associated with biochemical markers such as isozyme has limited their potential use. Currently, molecular markers [16][17][18] , especially microsatellite markers, have been widely used in many fish species for analyzing genetic diversity [19][20][21] , mapping of genes [22] , and screening of sex-specific markers [23] because of their many ideal traits, such as high polymorphism, ease of assay, and exhibition of codominant Mendelian inheritance. Microsatellite markers have been isolated in barfin flounder (Verasper moseri) [24] , and the genetic population diversity and interindividual relatedness for broodstock management were evaluated [25,26] .…”

Isolation and characterization of 31 polymorphic microsatellite markers in barfin flounder (Verasper moseri) and the cross-species amplification in spotted halibut (Verasper variegatus)

“…Finally, 82 pairs of primers were designed based on the microsatellite sequences from the two repeat-enriched libraries and seven pairs of primers were designed based on five genes (AB051424, AB051425, AB181833, AB287291 and AB287292) successfully. Polymerase chain reaction (PCR) were performed as described by Chen et al (2007) on a Peltier Thermal Cycler in 25 ll total volume that included 0.4 lM each primer, 0.2 mM each dNTP, 19 PCR buffer, 1.5 mM MgCl 2 , 0.75 unit Taq polymerase, and approximately 100 ng template DNA under the following conditions: one cycle of denaturation at 94°C for 4 min; 30 cycles of 30 s at 94°C, 50 s at a primer-specific annealing temperature (Table 1), and 50 s at 72°C. As a final step, products were extended for seven min at 72°C.…”

Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora)

“…Microsatellite markers are important tool to assess genetic diversity and to develop molecular breeding technique in fish because of their high level of polymorphism and co-dominant Mendelian inheritance (Chen et al 2005). However, there are no molecular markers such as microsatellite isolated and application in this species reported to date, in spite of many molecular markers including AFLP, SSR have been developed and used broadly in other fish species (Liu et al 2003;Chen et al 2007). Lack of sufficient and polymorphic microsatellite markers limited the development of genetic diversity researches and conservation for this species.…”