Isolation and Characterization of Pseudomonas sp. Cr13 and Its Application in Removal of Heavy Metal Chromium

Gong, Dafei; Ye, Fengcai; Pang, Caoping; Lu, Zujun; Shang, Changhua

doi:10.1007/s00284-020-02162-5

Cited by 15 publications

(6 citation statements)

References 52 publications

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“…Pseudomonas genus is versatile at many aspects such as plastics decomposing(Wilkes and Aristilde 2017), Cr 6+ absorbing(Gong et al . 2020), and Pseudomonas sp. Q18 , previously isolated from rotten wood in China, was used to degrade alkali lignin and raw lignocellulosic material(Yang et al .…”

Section: Resultsmentioning

confidence: 99%

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

Yang

Feng

et al. 2021

Letters Applied Microbiology

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In our study, we used 16SrRNA and ITS to investigate the microbial community composition and the effect of compound bacterial agent on the microbial community composition in the aerobic composting process of food waste (FW). At the bacterial level, the main phyla of Group A (compost naturally) were Proteobacteria and Firmicutes, and the main species were Pseudomonas_sp._GR7, Bacillus licheniformis and Pediococcus acidilactici. The main phyla of Group B (compost with compound bacterial agent) were Proteobacteria, Firmicutes and Streptophyta, and the main species were Klebsiella pneumoniae, Cronobacter sakazakii, Macrococcus caseolyticus, Enterococcus faecalis, Citrobacter freundii and Bacillus velezensis. It is worth noting that M. caseolyticus may be able to improve the effect of odour which is an important sensory index during aerobic composting. At the fungal level, the main phylum of both Groups A and B was Ascomycota, and the main species of Group A were Paecilomyces variotii, Byssochlamys spectabilis and Aspergillus fumigatus. The main species of Group B were Ogataea polymorpha and Millerozyma farinosa. Finally, the degradation rate of Group B was 81% that was about 15% higher than that of Group A, indicating that the compound bacterial agent could effectively improve the degradation rate and the composting process, while the low abundance of the compound bacterial agent in the composting process might be due to the small initial addition or the inhibition of other bacteria or fungi in the composting process.

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Section: Resultsmentioning

confidence: 99%

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

Yang

Feng

et al. 2021

Letters Applied Microbiology

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“…The Pseudomonas genus, Gram negative bacteria, are widely distributed in the nature, and the strain Pseudomonas sp. Cr13 can resist high concentration of Cr 6+ (up to 250 mg/L) and Cd 2+ (50 mg/L) [24]. Numerous studies have shown that Pseudomonas significantly alleviated Cr 6+ toxicity and enhanced the growth and nutrient uptake of plant as bionic bacteria [44].…”

Section: Discussionmentioning

confidence: 99%

“…Our laboratory had isolated a Pseudomonas sp. Cr13 strain with high Cr(VI) removal efficiency from Dachang town, Nandan county, Hechi city of Guangxi autonomous region [24]. LB medium (pH 7.0) was used in this study, which contained 10 g/L tryptone, 10 g/L NaCl, 5 g/L yeast extract [25].…”

Section: Heavy Metal Treatmentmentioning

confidence: 99%

“…In previous study, we isolated a strain Cr13 with significant Cr(VI) tolerance and Cr(VI) removal ability from mine-contaminated soils in Dachang town, Hechi city of Guangxi autonomous region, and this strain was identified as Pseudomonas genus by 16S rDNA sequencing [24]. We also used Biolog GEN III MicroStation to further identify this strain, but there was no result.…”

Section: Introductionmentioning

confidence: 99%

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Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

Pang

Zhang

et al. 2022

PLoS ONE

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Over exploitation of mineral resources has increasingly caused serious heavy metal contamination such as chromium (Cr). Cr(VI), the pathogenicity factor, is one of common environmental contaminants and widely known health hazards to living organisms. Therefore, it is urgent to control the polluted soil. Up to now, little is known about the regulatory mechanisms of Cr response in Pseudomonas sp. Cr13. In this study, transcriptome and differentially expressed genes in Pseudomonas sp. Cr13 strain was characterized by a comparison between Cr(VI)-treated sample and control sample using transcriptome sequencing approach. In total, 2974 genes were annotated, including 1245 (1154 down-regulated genes and 91 up-regulated genes) differentially expressed genes (DEGs). All DEGs could be assigned to 29 pathways, of which pathways related to amino acid metabolism, carbohydrate metabolism, energy metabolism and signal transduction mechanism were significantly enriched in Pseudomonas sp. Cr13. A possible mechanism for Cr toxicity response might be an active efflux which utilized a heavy metal translocating P-type ATPase to lower the intracellular Cr concentration. The down-regulated genes related to the antioxidant defense system had a key role in Cr reduction, such as SodA, Gst, osmC, BtuE, KatE, csdA and AhpC. The proteins that were visibly up-regulated, were likely to involve in alleviating Cr(VI) stress, and the significantly down-regulated genes such as MarR, Lrp, FhlA, GntR, HrcA, LysR family genes, were likely to reduce Cr(VI) induced oxidative stress. In addition, real-time quantitative PCR was used to analyze the expression patterns of some Cr responsive genes. This study reported the first identification of Cr responsive genes, and inferred the underlying regulatory mechanisms of response to Cr(VI) stress in Pseudomonas sp. Cr13.

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“…Pseudomonas sp. Cr13 can tolerate high concentrations of Cr(VI) and thus it partially removes Cr(VI) [134,135]. Recently, Acinetobacter sp.…”

Section: Chromium Remediationmentioning

confidence: 99%

The Double Face of Metals: The Intriguing Case of Chromium

et al. 2021

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Chromium (Cr) is a common element in the Earth’s crust. It may exist in different oxidation states, Cr(0), Cr(III) and Cr(VI), with Cr(III) and Cr(VI) being relatively stable and largely predominant. Chromium’s peculiarity is that its behavior relies on its valence state. Cr(III) is a trace element in humans and plays a major role in glucose and fat metabolism. The beneficial effects of Cr(III) in obesity and types 2 diabetes are known. It has been long considered an essential element, but now it has been reclassified as a nutritional supplement. On the other hand, Cr(VI) is a human carcinogen and exposure to it occurs both in occupational and environmental contexts. It induces also epigenetic effects on DNA, histone tails and microRNA; its toxicity seems to be related to its higher mobility in soil and swifter penetration through cell membranes than Cr(III). The microorganisms Acinetobacter sp. Cr1 and Pseudomonas sp. Cr13 have been suggested as a promising agent for bioremediation of Cr(VI). This review intends to underline the important role of Cr(III) for human health and the dangerousness of Cr(VI) as a toxic element. The dual and opposing roles of this metal make it particularly interesting. An overview of the recent literature is reported in support.

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Isolation and Characterization of Pseudomonas sp. Cr13 and Its Application in Removal of Heavy Metal Chromium

Cited by 15 publications

References 52 publications

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

Identification of differentially expressed genes for Pseudomonas sp. Cr13 stimulated by hexavalent chromium

The Double Face of Metals: The Intriguing Case of Chromium

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