Isolation and Characterization of Pseudomonas Fluorescens and It's Siderophores Production

Abstract

Production of the crop is affected by deficiency of fertilizers and low number of plant growth promoting rhizobacteria in soil. Pseudomonas fluorescens isolated from soil characterized and evaluated in Arachis hypogaea Pannapatti Salem,Tamil Nadu.Nigella sativa (Kolinji) Periyar University Salem,Tamil Nadu. were isolated and the isolates was named as (PSO1and PSO2)Bio chemical characterized showed that the isolates were acid procedures and positive to catalase and nitrate reductase production and do not have hydrogen sulphide production .The isolates were the amount of phosphate solubilization andsiderophores production by Pseudomonas fluorescens compared to isolates (PS01 and PS02).Maximum activity shows in PS01.The isolates were identified by biochemical test confirmed with genomic DNA identification. Arachis hypogaea seed with Pseudomonas fluorescens PS01 for plant growth promoting activities in green house showed a positive result. This isolates increased Arachis hypogaea leaves number, branches number, root length, and shoot length number of nuts also increased. In conclusion that the use of Pseudomonas fluorescensPS01 isolates could increase the Arachis hypogaea growth and yield performance. This isolates can used as potential bio fertilizers and plant growth promoter.