Isolation and Characterization of Root-Associated Bacterial Endophytes and Their Biocontrol Potential against Major Fungal Phytopathogens of Rice (Oryza sativa L.)

Khaskheli, Maqsood Ahmed; Wu, Lijuan; Chen, Guoqing; Chen, Long; Hussain, Sajid; Song, Dandan; Liu, Sihui

doi:10.3390/pathogens9030172

Cited by 37 publications

(28 citation statements)

References 70 publications

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“…Most of the bacteria isolated in our study from maize roots are known from the literature as rhizobacteria, many with a record as biocontrol agents on various crops including maize (e.g. Dardanelli et al 2012, Figueroa-López et al 2016, Shafi et al 2017, Khaskheli et al 2020. In addition to P. chlororaphis MA 342, Streptomyces antimycoticus FZB53-an agent with known anti-Fusarium activity (Koch and Löffler 2009)-and the model strain B. velezensis FZB42 (Fan et al 2018) were included as reference.…”

Section: Discussionmentioning

confidence: 99%

Selection of bacteria and fungi for control of soilborne seedling diseases of maize

et al. 2021

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Plant-based screening experiments were conducted with the aim of identifying biocontrol bacteria and fungi for seed treatment of maize. Candidate microorganisms were evaluated for their protective effects against soilborne infections by species of Fusarium, Globisporangium (syn. Pythium) and Rhizoctonia. The microorganisms tested were bacteria and fungi from maize roots or other sources, including some active microbial components of commercial biocontrol products. Due to the method of isolation chosen, the majority of bacteria from maize roots were spore formers, most of them species of the genera Bacillus,Brevibacillus and Paenibacillus. In pot tests with the potting substrate inoculated with F. culmorum, the level of control provided by seed treatment with the most efficacious bacterial and fungal isolates was comparable or close to the chemical reference seed treatment thiram. The most effective bacteria were species of Pseudomonas, Burkholderia and Streptomyces. Among a subset of approx. 100 bacteria studied, the in vivo and in vitro activities against F.culmorum were only weakly correlated, although some strains deviated from this pattern. The most effective fungi were two strains of Clonostachys rosea and isolates of Trichoderma. The latter and a strain of Gliocladium virens provided also protection against R.solani. Activity against Globisporangium ultimum was recorded for one isolate of Trichoderma and the two strains of C. rosea. A reduction in the impact of seedborne F. culmorum was also observed after seed treatment with two strains of F. oxysporum f. sp. strigae. The results are discussed in relation to previous reports on rhizosphere bacteria of maize and their use in biocontrol of plant pathogens or for plant growth promotion.

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Section: Discussionmentioning

confidence: 99%

Selection of bacteria and fungi for control of soilborne seedling diseases of maize

et al. 2021

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“…Previous studies show that Pseudomonas are known to increase plant resistance to stress and pathogens [ 51 , 85 , 86 , 87 ]; however, to our knowledge, no studies on trees or with P. tremulae have been conducted thus far, though several older studies demonstrated the biocontrol capabilities of fungi against this pathogen [ 14 , 22 , 23 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes

et al. 2021

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Aspens (Populus tremula and its hybrids), economically and ecologically important fast-growing trees, are often damaged by Phellinus tremulae, a rot-causing fungus. Plant-associated bacteria can be used to increase plant growth and resistance; however, no systematic studies relating the activity of symbiotic bacteria to aspen resistance against Phellinus tremulae have been conducted so far. The present pioneer study investigated the responses of two Populus tremula and two P. tremula × P. tremuloides genotypes to in vitro inoculations with, first, either Pseudomonas sp. or Paenibacillus sp. bacteria (isolated originally from hybrid aspen tissue cultures and being most closely related to Pseudomonas oryzihabitans and Paenibacillus tundrae, respectively) and, in the subsequent stage, with Phellinus tremulae. Both morphological parameters of in vitro-grown plants and biochemical content of their leaves, including photosynthesis pigments and secondary metabolites, were analyzed. It was found that both Populus tremula × P. tremuloides genotypes, whose development in vitro was significantly damaged by Phellinus tremulae, were characterized by certain responses to the studied bacteria: decreased shoot development by both Paenibacillus sp. and Pseudomonas sp. and increased phenol content by Pseudomonas sp. In turn, these responses were lacking in both Populus tremula genotypes that showed in vitro resistance to the fungus. Moreover, these genotypes showed positive long-term growth responses to bacterial inoculation, even synergistic with the subsequent fungal inoculation. Hence, the studied bacteria were demonstrated as a potential tool for the improved in vitro propagation of fungus-resistant aspen genotypes.

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“…Additionally, despite not being the focus of the present review, the potential of bacterial endophytes is noteworthy. Similar to what has been discussed, Khaskheli et al [ 141 ] addressed the importance of root-associated bacterial endophytes from rice plants to control its major diseases. They followed a similar approach and we recommend their work for additional material.…”

Section: Endophytes As Biocontrol Agentsmentioning

confidence: 93%

Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses

et al. 2021

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Plant diseases cause losses of approximately 16% globally. Thus, management measures must be implemented to mitigate losses and guarantee food production. In addition to traditional management measures, induced resistance and biological control have gained ground in agriculture due to their enormous potential. Endophytic fungi internally colonize plant tissues and have the potential to act as control agents, such as biological agents or elicitors in the process of induced resistance and in attenuating abiotic stresses. In this review, we list the mode of action of this group of microorganisms which can act in controlling plant diseases and describe several examples in which endophytes were able to reduce the damage caused by pathogens and adverse conditions. This is due to their arsenal of molecules generated during the interaction by which they form a kind of biological shield in the plant. Furthermore, considering that endophytic fungi can be an important tool in managing for biotic and abiotic stresses due to the large amount of biologically active substances produced, bioprospecting this class of microorganisms is tending to increase and generate valuable products for agriculture.

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Isolation and Characterization of Root-Associated Bacterial Endophytes and Their Biocontrol Potential against Major Fungal Phytopathogens of Rice (Oryza sativa L.)

Cited by 37 publications

References 70 publications

Selection of bacteria and fungi for control of soilborne seedling diseases of maize

Selection of bacteria and fungi for control of soilborne seedling diseases of maize

Impact of Plant-Associated Bacteria on the In Vitro Growth and Pathogenic Resistance against Phellinus tremulae of Different Aspen (Populus) Genotypes

Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses

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