Isolation and characterization of rubisco small subunit gene promoter from common wheat (<i>Triticum aestivum</i>L.)

Mukherjee, Shalini; Stasolla, Claudio; Brûlé‐Babel, Anita L.; Ayele, Belay T.

doi:10.4161/15592324.2014.989033

Cited by 22 publications

(20 citation statements)

References 34 publications

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“…Biotechnology programs are at the forefront of agricultural research and adopting techniques such as genetic engineering and genome editing for endogenous genes manipulation [227][228][229] is key to tackle these issues. To generate plants with sustainable increases in yields and nutritional quality, and with the ability to adapt successfully to changing environmental conditions, will require a multi-targeted approach touching upon multiple aspects of carbon assimilation, light adaptation and nutrient biosynthesis and will require new tools including vectors for multiple gene insertion [230][231][232][233][234] and tissue-specific promoters [235][236][237][238][239][240]. If the promise of these biotechnology programs is to be realized, public perception of genetic modification and genome editing technologies will need to be addressed.…”

Section: Future Prospects and Conclusionmentioning

confidence: 99%

Genetic Engineering for Global Food Security: Photosynthesis and Biofortification

Simkin

2019

Plants

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Increasing demands for food and resources are challenging existing markets, driving a need to continually investigate and establish crop varieties with improved yields and health benefits. By the later part of the century, current estimates indicate that a >50% increase in the yield of most of the important food crops including wheat, rice and barley will be needed to maintain food supplies and improve nutritional quality to tackle what has become known as ‘hidden hunger’. Improving the nutritional quality of crops has become a target for providing the micronutrients required in remote communities where dietary variation is often limited. A number of methods to achieve this have been investigated over recent years, from improving photosynthesis through genetic engineering, to breeding new higher yielding varieties. Recent research has shown that growing plants under elevated [CO2] can lead to an increase in Vitamin C due to changes in gene expression, demonstrating one potential route for plant biofortification. In this review, we discuss the current research being undertaken to improve photosynthesis and biofortify key crops to secure future food supplies and the potential links between improved photosynthesis and nutritional quality.

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Section: Future Prospects and Conclusionmentioning

confidence: 99%

Genetic Engineering for Global Food Security: Photosynthesis and Biofortification

Simkin

2019

Plants

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“…Additionally, the semi-constitutive rice tungro virus promoter [ 37 , 38 ] has been used to express genes in the aerial parts of the wheat plant [ 9 ]. More recently, the rubisco small subunit (Rubisco) gene promoter from wheat was shown to direct expression in immature wheat embryos and tobacco leaves in transient assays [ 39 ]. Although this demonstrates the possibility that wheat promoters can be used to target transcripts to photosynthetic tissues, further work is needed to demonstrate that the wheat Rubisco promoter can function in leaves in stable transgenic wheat plants.…”

Section: Introductionmentioning

confidence: 99%

Identification of Leaf Promoters for Use in Transgenic Wheat

Alotaibi

Sparks

Parry

et al. 2018

Plants

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Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields.

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“…In higher plants, it is composed of 8 large subunits and 8 small subunits both arranged in 4 dimers. The large subunits of RuBisCO (rbcL) are encoded in the chloroplast whereas the small subunits (rbcS) are encoded in the nucleus (ELLIS, 1979;MUKHERJEE et al, 2015). In addition to attracting interest because of its importance in photosynthesis, studies of RuBisCO continue to make major contributions in our understanding of plant diversity, chloroplast biogenesis, the coordination and regulation of gene expression in the chloroplast and nucleus, and chloroplast import and processing of nuclearencoded proteins (PORTIS, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and analysis of RuBisCO large subunit proteins in Morus L. (Moraceae) species

Sevindik

2018

Genetika

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In this study, we performed sequence, physicochemical, phylogenetic, and comparative 3D (three-dimensional) analyses of RuBisCO large subunit proteins of the Morus species using various bioinformatics tools. The sequence lengths of the RuBisCO proteins varied between 140 and 475 amino acids with molecular weights (MW) ranging from 15463.40 to 52667.95 Da. The most acidic protein sequences were in M. australis, M. rotundiloba, M. serrata, and M. cathayana (pI=4.77). The extinction coefficients of the proteins at 280 nm ranged from 21430 to 69830 M-1 cm-1 , the instability index (II) values ranged from 26.06 to 42.17, and the GRAVY values ranged from-0.355 to-0.215. The most abundant amino acid of RuBisCO proteins was Gly (9.5%) while the least abundant ones were Cys and Trp (1.5%). Based on the phylogenetic analysis, the tree constructed using RuBisCO proteins is composed of three main clades. A RAMPAGE analysis revealed that 96.1%-98.5% of residues were located in the favored region in RuBisCO proteins. To predict the three dimensional (3D) structures of the RuBisCO proteins PyMOL was used. The highest number of Gly residues was detected in M.mongolica, M. indica, M.notabilis, M. alba var. atropurpurea and M. alba var. multicaulis, while the least Gly residues were detected in M. australis, M.rotundiloba, M. serrata and M.cathayana. The results of our study provide insights into fundamental characteristic of RuBisCO proteins in Morus species.

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Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivumL.)

Cited by 22 publications

References 34 publications

Genetic Engineering for Global Food Security: Photosynthesis and Biofortification

Genetic Engineering for Global Food Security: Photosynthesis and Biofortification

Identification of Leaf Promoters for Use in Transgenic Wheat

Genome-wide identification and analysis of RuBisCO large subunit proteins in Morus L. (Moraceae) species

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