Isolation and Characterization of Salt Tolerant Bacteria from Saline Areas of Khyber Pakhtunkhwa

Abstract

The survival of microbes in various severe condition leads to developments in the medical, agricultural, and bioenergy sectors. In this study we isolate, identify and characterize salt tolerant bacteria from saline areas. Sample was taken from hyper saline areas of KP. Four bacterial isolates were isolated from sample and different techniques were applied. All the bacterial isolates were able to grow on agar media in the presence of salt this shows the strict halotolerant nature of isolates. Serial dilution was performed to check the salt concentration at which minimum and maximum growth occurs. The growth of isolates was inversely proportional to salt concentration in media as the concentration of salt in media was increases the growth of isolates were decreases and vice versa. Only one isolate was able to show resistance to 2.5M salt in media and 3 isolates were able to show resistance to 2M salt in media. Gram staining was performed to check that the isolates are gram positive or gram negative; all of the isolates were gram positive. For molecular analysis genomic DNA was extracted and checks the result on gel.

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