Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3

Moriguchi, Mitsuaki; Sakai, Kenji; Tateyama, Ryoji; Furuta, Yoichi; Wakayama, Mamoru

doi:10.1016/0922-338x(94)90143-0

Cited by 71 publications

(39 citation statements)

References 16 publications

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“…Marine microorganisms have the important characteristic of salt tolerance (4), which renders use of them or their enzymes important in industrial processes that require high salt environments. Recently, we reported the production of salt-tolerant L-glutaminase by the marine fungus Beauveria bassiana BTMF S-10 under solid-state fermentation on polystyrene as an inert support (5).…”

Section: Introductionmentioning

confidence: 99%

Continuous Production of Extracellular L-Glutaminase by Ca-Alginate-Immobilized Marine Beauveria bassiana BTMF S-10 in Packed-Bed Reactor

Sabu

Kumar

Chandrasekaran

2002

ABAB

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L-Glutamine amidohydrolase (L-glutaminase, EC 3.5.1.2) is a therapeutically and industrially important enzyme. Because it is a potent antileukemic agent and a flavor-enhancing agent used in the food industry, many researchers have focused their attention on L-glutaminase. In this article, we report the continuous production of extracellular L-glutaminase by the marine fungus Beauveria bassiana BTMF S-10 in a packed-bed reactor. Parameters influencing bead production and performance under batch mode were optimized in the order-support (Na-alginate) concentration, concentration of CaCl 2 for bead preparation, curing time of beads, spore inoculum concentration, activation time, initial pH of enzyme production medium, temperature of incubation, and retention time. Parameters optimized under batch mode for L-glutaminase production were incorporated into the continuous production studies. Beads with 12 × 10 8 spores/g of beads were activated in a solution of 1% glutamine in seawater for 15 h, and the activated beads were packed into a packed-bed reactor. Enzyme production medium (pH 9.0) was pumped through the bed, and the effluent was collected from the top of the column. The effect of flow rate of the medium, substrate concentration, aeration, and bed height on continuous production of L-glutaminase was studied. Production was monitored for 5 h in each case, and the volumetric productivity was calculated. Under the optimized conditions for continuous production, the reactor gave a volumetric productivity of 4.048 U/(mL·h), which indicates that continuous production of the enzyme by Ca-alginate-immobilized

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Section: Introductionmentioning

confidence: 99%

Continuous Production of Extracellular L-Glutaminase by Ca-Alginate-Immobilized Marine Beauveria bassiana BTMF S-10 in Packed-Bed Reactor

Sabu

Kumar

Chandrasekaran

2002

ABAB

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“…It retained 90% of activity at 10% NaCl concentration and 75% activity upto 15% NaCl, compared to the glutaminase -I from marine Micrococcus sp. which recorded 100% activity at 10-16% NaCl (Moriguchi et al, 1994). Whereas glutaminase from non-marine microbes were found to be inactivated at high salt concentrations, limiting their industrial potential.…”

Section: Resultsmentioning

confidence: 99%

Purification and characterization of an anti-cancer enzyme produced by marine Vibrio Costicola under a novel solid state fermentation process

Prabhu

Chandrasekaran

1999

Braz. arch. biol. technol.

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“…An intracellular glutaminase from Cryptococcus albidus preferred an optimal pH of 5.5-8.5 20 .Whereas, glutaminase 1 and 11 isolated from marine Micrococcus luteus were active at alkaline pH values of 8.0 and 8.5 respectively 35 .…”

Section: Effect Of Phmentioning

confidence: 99%

“…Glutaminase I&II from Micrococcus luteus had a temperature optima of 50°C and the presence of NaCl (10%) increased the 16 thermo stability 35 . The optimum temperature for activity of both intra and extracellular glutaminases from A. oryzae was 45°C while they became inactive at 55°C 9 .Glutaminase isolated from Penicillium brevicompactum NRC 829 showed its maximal activity at 50 0 C, further increase in temperature at 70 0 C retained its activity indicates its thermostable nature 57.…”

Section: Effect Of Phmentioning

confidence: 99%

Untitled

2014

IJPSR

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ABSTRACT:One of the prime candidates in the treatment of debilitating human cancers includes a family of enzymes referred to as L-glutaminases. The antitumor activity of these enzymes found effective in countering Acute Lymphoblastic Leukemia (ALL a commonly diagnosed pediatric cancer.). .Apart from therapeutic use, it is having a number of other applications like in food industry, analysis and in production of fine chemicals. This review, hence, mainly focuses on the biochemical aspects of L-Glutaminase production, aiming to comprehend the physiochemical characteristics, application of LGlutaminase and its properties. Processes central to these biochemical aspects, including submerged fermentation and Solid state fermentation of Lglutaminase producing organisms are also discussed.

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Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3

Cited by 71 publications

References 16 publications

Continuous Production of Extracellular L-Glutaminase by Ca-Alginate-Immobilized Marine Beauveria bassiana BTMF S-10 in Packed-Bed Reactor

Continuous Production of Extracellular L-Glutaminase by Ca-Alginate-Immobilized Marine Beauveria bassiana BTMF S-10 in Packed-Bed Reactor

Purification and characterization of an anti-cancer enzyme produced by marine Vibrio Costicola under a novel solid state fermentation process

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