Isolation and characterization of SGF3, a novel Microviridae phage infecting Shigella flexneri

Lu, Haiquan; Xiong, Wenbin; Zong, Li; Yan, Peihan; Liu, Ruyin; Liu, Xinchun

doi:10.1007/s00438-022-01883-5

Cited by 5 publications

(5 citation statements)

References 43 publications

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“…Members of the the Gokushovirinae only infect Chlamydia, Bdellovibrio and Spiroplasma(13, 72). However, an increasing number of studies indicate that microviruses can infect a broader range of bacterial hosts, including Vibrio parahaemolyticus(73, 74), Salmonella(75), Shigella flexneri(76) and other bacteria. To address the question of infecting hosts, this study employed two new host prediction methods.…”

Section: Discussionmentioning

confidence: 99%

Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse

Xie,

Lin,

Zhu

et al. 2024

Preprint

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Microviridae is a family of phages with circular ssDNA genomes that are widely found in various environments and organisms. In this study, Virome techniques were employed to explore potential members of Microviridae in poultry slaughterhouse, leading to the identification of 98 novel and complete microvirus genomes. Using a similarity clustering network classification approach, these viruses were found to belong to at least 6 new subfamilies within Microviridae and 3 higher-level taxonomic units. Analysis of their genomes found that the genome size, GC content and genomic structure of these new taxa showed obvious regularities, validating the rationality of our classification method. Compared with the 19 families classified by previous researchers for microviruses dataset, our method can divide microviruses into about 45 more detailed clusters, which may serve as a new standard for classifying Microviridae members. Furthermore, addressing the scarcity of host information for microviruses, this study significantly broadened their host range and discovered over 20 possible new hosts, including important pathogenic bacteria such as Helicobacter pylori and Vibrio cholerae, as well as different taxa demonstrated distinct host specificity. The findings of this study effectively expand the diversity of the Microviridae, providing new insights for their classification and identification. Additionally, it offers a fresh perspective for monitoring and controlling pathogenic microorganisms in poultry slaughterhouse environments.

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Section: Discussionmentioning

confidence: 99%

Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse

Xie,

Lin,

Zhu

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Members of the Gokushovirinae only infect Chlamydia , Bdellovibrio and Spiroplasma ( Brentlinger et al, 2002 ; King et al, 2012 ). However, an increasing number of studies indicate that microviruses can infect a broader range of bacterial hosts, including Vibrio parahaemolyticus ( Guo et al, 2022 ; Xu et al, 2022 ), Salmonella ( Li et al, 2021 ), Shigella flexneri ( Lu et al, 2022 ) and other bacteria. Predicting the hosts of these viruses is challenging because many cellular organisms lack genomic data or have not been extensively studied for their viruses.…”

Section: Discussionmentioning

confidence: 99%

Identification and classification of the genomes of novel microviruses in poultry slaughterhouse

Xie,

Lin,

Sun

et al. 2024

Front. Microbiol.

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Microviridae is a family of phages with circular ssDNA genomes and they are widely found in various environments and organisms. In this study, virome techniques were employed to explore potential members of Microviridae in a poultry slaughterhouse, leading to the identification of 98 novel and complete microvirus genomes. Using a similarity clustering network classification approach, these viruses were found to belong to at least 6 new subfamilies within Microviridae and 3 higher-level taxonomic units. Genome size, GC content and genome structure of these new taxa showed evident regularities, validating the rationality of our classification method. Our method can divide microviruses into about 45 additional detailed clusters, which may serve as a new standard for classifying Microviridae members. Furthermore, by addressing the scarcity of host information for microviruses, the current study significantly broadened their host range and discovered over 20 possible new hosts, including important pathogenic bacteria such as Helicobacter pylori and Vibrio cholerae, as well as different taxa demonstrated different host specificities. The findings of this study effectively expand the diversity of the Microviridae family, providing new insights for their classification and identification. Additionally, it offers a novel perspective for monitoring and controlling pathogenic microorganisms in poultry slaughterhouse environments.

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“…Phages SSE1, SGF2, and SGF3 were isolated from S. dysenteriae 1.1869, S. flexneri 1.1868, and S. flexneri 1.10599, respectively [ 22 – 24 ]. They were all stored in -80℃ mixed with glycerol.…”

Section: Methodsmentioning

confidence: 99%

“…Similar to phage cocktails, phage lytic proteins combine with other antimicrobials, such as antibiotics, to target different receptors. Three phages, SSE1, SGF2, and SGF3, obtained in a previous study [ 22 – 24 ] all had cleavage activity against Shigella spp . The encoded lytic proteins LysSSE1 and HolSSE1 were annotated as lysozyme and holin, respectively, based on the NCBI database alignment.…”

Section: Introductionmentioning

confidence: 99%

Synergistic action of phages and lytic proteins with antibiotics: a combination strategy to target bacteria and biofilms

Zong

El-Baz

2023

BMC Microbiol

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Background Multidrug-resistant bacteria continue to emerge owing to the abuse of antibiotics and have a considerable negative impact on people and the environment. Bacteria can easily form biofilms to improve their survival, which reduces the efficacy of antibacterial drugs. Proteins such as endolysins and holins have been shown to have good antibacterial activity and effectively removal bacterial biofilms and reduce the production of drug-resistant bacteria. Recently, phages and their encoded lytic proteins have attracted attention as potential alternative antimicrobial agents. The aim of the present study was to investigate the sterilising efficacy of phages (SSE1, SGF2, and SGF3) and their encoded lytic proteins (lysozyme and holin), and to further explore their potential in combination with antibiotics. To the ultimate aim is to reduce or replace the use of antibiotics and provide more materials and options for sterilisation. Results Phages and their encoded lytic proteins were confirmed to have great advantages in sterilisation, and all exhibited significant potential for reducing bacterial resistance. Previous studies on the host spectrum demonstrated the bactericidal efficacy of three Shigella phages (SSE1, SGF2, and SGF3) and two lytic proteins (LysSSE1 and HolSSE1). In this study, we investigated the bactericidal effects on planktonic bacteria and bacterial biofilms. A combined sterilisation application of antibiotics, phages, and lytic proteins was performed. The results showed that phages and lytic proteins had better sterilisation effects than antibiotics with 1/2 minimum inhibitory concentrations (MIC) and their effect was further improved when used together with antibiotics. The best synergy was shown when combined with β- lactam antibiotics, which might be related to their mechanism of sterilising action. This approach ensures a bactericidal effect at low antibiotic concentrations. Conclusions This study strengthens the idea that phages and lytic proteins can significantly sterilise bacteria in vitro and achieve synergistic sterilisation effects with specific antibiotics. Therefore, a suitable combination strategy may decrease the risk of drug resistance.

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Isolation and characterization of SGF3, a novel Microviridae phage infecting Shigella flexneri

Cited by 5 publications

References 43 publications

Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse

Identification and classification of the genomes of novel Microviruses in poultry slaughterhouse

Identification and classification of the genomes of novel microviruses in poultry slaughterhouse

Synergistic action of phages and lytic proteins with antibiotics: a combination strategy to target bacteria and biofilms

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