Isolation and Characterization of Soil Bacteria from an Abandoned Coal Mine in Southeast Kansas

Bechtold, Rachel; Sharma, N. K.; Vega, Michael; Datta, Saugata; Arruda, Joseph A.; Ghosh, Anuradha

doi:10.1660/062.123.0305

Transactions of the Kansas Academy of Science

2020

DOI: 10.1660/062.123.0305

|View full text |Cite

Isolation and Characterization of Soil Bacteria from an Abandoned Coal Mine in Southeast Kansas

Rachel Bechtold

N. K. Sharma

Michael Vega

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2023

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Bacterial succession in an abandoned circum-neutral opencast coal mine in India

Banerjee,

Misra,

Sar

et al. 2023

Soil Res.

View full text Add to dashboard Cite

Context Abandoned mines provide an ideal opportunity to study the succession of microbial communities, which is crucial for the development and stability of the soil. Aims We examined the diversity and temporal succession of the microbial community in an abandoned coal mine. Methods We investigated soil geochemical parameters and microbial succession using high-throughput 16S rRNA gene amplicon sequences over a 4-year period in a derelict opencast circum-neutral coal mine in Tasra colliery, Jharkhand, India. Results Soil pH (4.46–6.13), organic carbon (0.86–4.53%), available nitrogen (0.001–0.003%) and total cultivable heterotrophic microbial load increased, and concentrations of chloride (24 865–15 067 ppm), sulfate (26 417–417 ppm) and heavy metals reduced over the period of 4 years. The 16S rRNA gene sequences identified Proteobacteria as the dominant phylum across samples except for the fourth year, where Actinobacteria was predominant. Chlorobi, WPS-2 and Spirochaetes were exclusively identified in the first year. Twelve phyla present in the first year were completely absent by the end. In the fourth year, an abundance of nitrogen-fixing members such as Burkholderiales, Rhizobiales, Roseiflexales and Actinomycetales was noted and semi-quantitative PCR suggested that nitrogenases were predominately vanadium dependent (relative to molybdenum). Gradual shifts from purportedly chemolithotrophic to heterotrophic metabolic strategies were also observed. Conclusion This work establishes that soil improvement in abandoned coal mines involves the dynamic interplay of physicochemical parameters and shifts in microbial communities and dominant metabolic guilds. This knowledge can be used to plan and monitor remediation measures in such sites.

show abstract

Bacterial succession in an abandoned circum-neutral opencast coal mine in India

Banerjee,

Misra,

Sar

et al. 2023

Soil Res.

View full text Add to dashboard Cite

show abstract

Isolation and Characterization of Micro-organisms found in Lignite Coal in Bharuch Mines of Gujarat

Udeet Ranjan Das,

Meghna Goswami,

Priyanka Patel

2023

IJSRST

View full text Add to dashboard Cite

Gujarat provides a rich source of high-grade lignite coal accomplishing the fuel needs of the national economy, under Gujarat Mineral Development Corporation Ltd. However, the lignite mines in Bharuch district of Gujarat state are reservoirs to a huge microbial diversity, which might be important for various industrial applications. The present study focuses on the isolation and characterization of microorganisms found in the lignite coal from the Bharuch mines of Gujarat. The samples were resuspended in different diluents – water and Phosphate Buffered Saline and cultured on Nutrient Agar. Morphological characterization was done for the isolated strains followed by biochemical characterization for microbial identification. Based on the observations, two species were identified probably as Streptococcus sp. and Staphylococcus sp. on biochemical characterizations. The microbial isolates were further screened for their ability to produce lipase enzyme by qualitative screening tests for lipase production by Phenol Red Olive Oil Agar. All the isolates were tested positive for Lipase enzyme production were subjected to Tween-80 Hydrolysis tests for confirmation. This marks their potential to be studied for development of bioremediation strategies for crude oil contamination in soil or water bodies. This was followed by reviewing the use of such isolates for other applications including antioxidant studies, development of biodegradation strategies, etc.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Isolation and Characterization of Soil Bacteria from an Abandoned Coal Mine in Southeast Kansas

Cited by 2 publications

References 36 publications

Bacterial succession in an abandoned circum-neutral opencast coal mine in India

Bacterial succession in an abandoned circum-neutral opencast coal mine in India

Isolation and Characterization of Micro-organisms found in Lignite Coal in Bharuch Mines of Gujarat

Contact Info

Product

Resources

About