Isolation and characterization of starch degrading bacteria from disparate soil samples

Srivathsan, Vidhyutha; Bhandari, Mahima; Swaminathan, Priya

doi:10.7324/jabb.2022.100524

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…The PCR started with 5 min denaturation at 95°C, followed by 35 cycles of 30 s denaturation at 94°C, 30 sec annealing at 60°C, 1 min extension at 72°C, and 10 min final extension at 72°C [17]. A total of 5 µL of the product were analyzed using 1% agarose gel dissolved in 1X TAE buffer, and the gel electrophoresis was carried out for 25 min at 90 V. The presence of the DNA band was visualized and observed under UV light using a gel documentation system [18]. Each isolate's PCR product was then compared to a 1 kb DNA Ladder (Promega).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Rachmawati¹,

Asarina²,

Kennardi³

et al. 2022

J App Biol Biotech

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Antimicrobial peptides (AMPs) are ribosomal synthesized peptides produced by microorganisms, including thermophilic bacteria isolated from hot springs. The protein has a broad spectrum against Gram-positive and Gramnegative bacteria, making it a candidate for alternative antibiotics. This study aims to identify and characterize antimicrobial peptide coding genes of thermophilic bacteria isolated from Mount Kamojang and Tangkuban Perahu crater hot springs in West Java. Six samples of crater water were collected from different locations around Mount Kamojang and Tangkuban Perahu. All six isolates were identified using 16S rRNA gene sequencing and were identified as Geobacillus sp. The antagonist test of all isolates against Streptococcus mutans was carried out using a disk test, and it was seen that there was an inhibition zone formation in the culture of all strains. The largest inhibition zone was formed in the culture of Geobacillus strain TM6Sp1 which was 7.96 mm. The identification of AMP encoding genes was carried out by entering the identified genome sequence of samples into the BAGELA4 web server, and the NCBI database was used for sequence analysis and alignment. The results showed that all of the samples have the same alignment lanthipeptide Class A, which has a 100% similarity to the lantibiotic precursor. This indicates that thermophilic bacteria isolated from crater hot springs in the mountains surrounding West Java have antimicrobial activity against S. mutans due to their ability to produce AMPs of lanthipeptide Class A with an amplicon size of 230 base pairs.

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Section: Molecular Characterizationmentioning

confidence: 99%

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Rachmawati¹,

Asarina²,

Kennardi³

et al. 2022

J App Biol Biotech

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Isolation and Identification of Soil-Derived Amylase-Producing Bacteria

H.S,

G.L

et al. 2024

Curr Agri Res Jour

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Soil is a vital ecosystem for various life forms, including bacteria. Bacteria are the main group of soil microbes, mostly because of their ability to produce various extracellular enzymes. Amylases are the enzymes that break down starch, which are essential in biotechnology industries such as food, fermentation, textiles, and paper manufacturing. The demand for amylase is often high since many enterprises do not have access to local supplies of the enzyme. The objective of the current investigation is to isolate and identify bacterial isolates that can produce the enzyme amylase from soil samples. Soil samples were collected from the unexplored Biligiriranga hills (11.9988° N, 77.1398° E) in south-western Karnataka Bacterial isolates were isolated from soil samples and were primarily screened on starch agar medium to determine the amylase production. Three out of fifteen bacterial isolates that produced amylase were selected based on the highest clear zone around the bacterial colony. These three bacterial isolates were identified both microscopically and biochemically. Gram staining demonstrated that the isolates are rod-shaped and gram-positive organisms. Biochemical studies indicated that the isolates were positive for the catalase and starch hydrolysis tests, whereas negative for the potassium hydroxide and methyl red tests. Besides, 16S rRNA gene amplification and sequencing confirmed the isolate as Bacillus subtilis. The 16S rRNA sequence of B. subtilis strain B has been deposited in the NCBI GenBank. The optimal growth conditions observed in B. Subtilis. The optimal growth conditions observed in B. Subtilis were 37° C temperature at pH 7 and incubation period for 37h. Thus, the isolation and identification of soil-derived amylase-producing bacteria are crucial for understanding their potential applications in various industrial processes, such as bioremediation, waste management, and the production of enzymes for food, pharmaceuticals, and biofuel industries.

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Isolation of α-Amylase Producing Microorganisms from Soil of Kachchh, Gujarat

Morbia,

Pandey,

Mahla

et al. 2024

J. Pure Appl. Microbiol.

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The purpose of this study is to explore the soil of the Gujarat, Kachchh region to identify amylase-producing bacteria and characterize them using molecular methods. The unique ecological characteristics of the Kachchh region may facilitate the isolation of these bacteria. Samples were collected from multiple locations within the Kachchh District, including Gandhidham, Rapar, Bhuj, Nakhatrana, Mandvi, and Mundra Talukas. These samples were then screened to isolate amylase-producing bacteria. A total of 27 different types of colonies were identified, out of which 16 exhibited amylase production (M1-M16). Out of 27 colonies identified, 16 showed amylase production. Strains M2, M7, and M13 exhibited high amylase activity, with M2 showing a consistent increase over 72 hours, making it a strong candidate for amylase production. Further identification of M2 stain identified M2 as a Gram-positive, spore-forming, capsulated, and motile bacillus, specifically Bacillus licheniformis. This was confirmed through DNA sequencing and analysis in the NCBI database, which showed a 99.15% similarity with Bacillus licheniformis. The study concludes that Soil in Kachchh is rich with microorganisms that produce amylase, an enzyme with diverse industrial applications. These organisms are valuable for sectors like food, textiles, paper, detergents, pharmaceuticals, and biofuel production.

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Isolation and characterization of starch degrading bacteria from disparate soil samples

Cited by 3 publications

References 16 publications

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Antimicrobial peptide coding gene of thermophilic bacteria isolated from crater hot spring in mountains around West Java

Isolation and Identification of Soil-Derived Amylase-Producing Bacteria

Isolation of α-Amylase Producing Microorganisms from Soil of Kachchh, Gujarat

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