Isolation and characterization of starch-utilizing mutants of Escherichia coli.

Shibuya, Ichiro; Iimura, Yuzuru; Ishikawa, Takahiro; Ouchi, Kazuo; Matsuyama, Asahi; Yamamoto, Tetsuro; Morikawa, Minoru; Nishiya, Takamichi

doi:10.1271/bbb1961.50.875

Cited by 5 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shibuya et al [ 19 ] reported similar growth profiles for other adapted strains as well as extracellular amylolytic activity from the adapted strains. However, in the present work, the adapted strains did not show amylolytic activity because no starch degradation was observed (Figure 1 ).…”

Section: Resultsmentioning

confidence: 90%

Engineering Escherichia coli K12 MG1655 to use starch

Rosales-Colunga

Martı́nez-Antonio

2014

Microb Cell Fact

View full text Add to dashboard Cite

BackgroundTo attain a sustainable bioeconomy, fuel, or valuable product, production must use biomass as substrate. Starch is one of the most abundant biomass resources and is present as waste or as a food and agroindustry by-product. Unfortunately, Escherichia coli, one of the most widely used microorganisms in biotechnological processes, cannot use starch as a carbon source.ResultsWe engineered an E. coli strain capable of using starch as a substrate. The genetic design employed the native capability of the bacterium to use maltodextrins as a carbon source plus expression and secretion of its endogenous α-amylase, AmyA, in an adapted background. Biomass production improved using 35% dissolved oxygen and pH 7.2 in a controlled bioreactor.ConclusionThe engineered E. coli strain can use starch from the milieu and open the possibility of optimize the process to use agroindustrial wastes to produce biofuels and other valuable chemicals.

show abstract

Section: Resultsmentioning

confidence: 90%

Engineering Escherichia coli K12 MG1655 to use starch

Rosales-Colunga

Martı́nez-Antonio

2014

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…Total of 247 isolates were tested for -amylase production on agar plates containing soluble starch (Shibuya et al, 1986). Among them, total 231 amylase positive isolates were selected after application of iodine vapour.…”

Section: Resultsmentioning

confidence: 99%

“…(Lennete et al, 1985;Hamilton et al, 1999). The isolated strains were monitored for amylase production on agar plate composed of Na 2 HPO 4 6 g, KH 2 PO 4 3 g, NaCl 0.5 g, MgSO 4 0.24 g, CaCl 2 0.01 g, peptone 3 g, 1% (w/v) soluble starch (Merck), and Agar 15 g (Shibuya et al, 1986). The starting pH of the medium was 9.5 arranged with 10% Na 2 CO 3 after sterilization.…”

Section: Organisms and Cultivation Conditionsmentioning

confidence: 99%

Production and characterization of alkaliphilic alpha-amylase from Bacillus subtilis A10 isolated from soils of Kahramanmaras, Turkey

Aygan¹,

Sarıtürk²,

Köstekci³

et al. 2014

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Bacillus subtilis A10 isolated from orchard soil, Kahramanmaras, Turkey. The enzyme synthesis was observed between pH 7.0-11.0, with an optimum 37°C. The amylase was purified by fractional ammonium sulfate precipitation and sephadex G-100 column. Analysis of the enzyme with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a single band which was estimated as 46.9 kDa. The enzyme presented an optimum activity at pH 8.5 and 45°C. Its thermal stability between 20-50°C was about 89.5% for 30 min. The pH stability was observed between pH 7.0 and 10.0 with an average of 84.9% of retaining activity for 15min. The activity of the enzyme was inhibited by SDS and EDTA by 48.4 and 75.9%, respectively. On the other hand, Na 2 SO 3 and -Mercaptoethanol did not effect the enzyme activity. This alkaliphilic amylase is suitable for waste-paper, starch and bioethanol industries.

show abstract

“…Unfortunately, there has been little information concerning the genetic and physiological mechanism of carbon nutrient utilization in the heterotrophic marine bacterium, A. espejiana Bal-31. The wild type strain of E. coli could not grow in medium with added starch, whilst the starch-utilizing mutants were well adapted and were induced to synthesize intracellular a-amylase in maltose as well as starch-supplemented media (Shibuya et al, 1986). On the other hand, A. espejiana Bal-31 grew well and extracellular a-amylase was induced in starch-supplemented medium, whilst the S2a mutant grew well both in maltose and starch added media; however, induction of extracellular aamylase occurred in maltose rather than in starch-supplemented medium.…”

Section: Discussionmentioning

confidence: 99%

Maltose adaptive mutant of heterotrophic marine bacterium, Alteromonas espejiana Bal-31: Changes in the growth and the induction of extracellular .ALPHA.-amylase

Lee

Chou

et al. 2005

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Growth of the heterotrophic marine bacterium, Alteromonas espejiana Bal-31 was inhibited in the presence of sucrose, maltose and even glucose, but not with starch. Extracellular a a-amylase was induced with a lag phase of 2 h in the presence of starch. In contrast, cell growth of the S2a mutant was not affected by the addition of maltose, and starch was ineffective in the induction of extracellular a a-amylase in this mutant. Activity of extracellular a a-amylase was induced from the S2a mutant with a 4-h lag phase in the presence of maltose, and the high level of enzyme activity was maintained for at least 24 h. Activity of a a-amylase induced by both wild type starch and S2a mutant maltose cultures were mainly observed in extracellular locations. This activity could be stopped by tetracycline treatment, indicating that enzyme induction was dependant on gene expression and not on enzyme protein secretory mechanisms. Our results showed that the mutation in S2a changed the growth and the modulation of the specific a a-amylase in response to carbon nutrients.

show abstract

Isolation and characterization of starch-utilizing mutants of Escherichia coli.

Cited by 5 publications

References 2 publications

Engineering Escherichia coli K12 MG1655 to use starch

Engineering Escherichia coli K12 MG1655 to use starch

Production and characterization of alkaliphilic alpha-amylase from Bacillus subtilis A10 isolated from soils of Kahramanmaras, Turkey

Maltose adaptive mutant of heterotrophic marine bacterium, Alteromonas espejiana Bal-31: Changes in the growth and the induction of extracellular .ALPHA.-amylase

Contact Info

Product

Resources

About