Isolation and characterization of T cells from semen

Olivier, Abraham J.; Liebenberg, Lenine J.; Coetzee, David; Williamson, Anna‐Lise; Passmore, Jo‐Ann S.; Burgers, Wendy A.

doi:10.1016/j.jim.2011.11.001

Cited by 12 publications

(13 citation statements)

References 29 publications

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“…Semen CD4+ T cells have a profile typical of resident mucosal T cells [16]; they are mostly central memory cells (CD95-+CD28+), and a large proportion express CD69, an activation marker highly expressed at all stages of infection. This phenotype is very close to the one described for T cells present in human semen [17], which also strongly express CD69 and CD38. The differentiation and activation status makes these cells particularly well equipped to replicate the virus.…”

Section: Leukocytes In the Semen Of Siv-infected Macaquessupporting

confidence: 83%

Nonhuman Primate Models for Cell-Associated Simian Immunodeficiency Virus Transmission: The Need to Better Understand the Complexity of HIV Mucosal Transmission

Bernard-Stoecklin

Gommet

Cavarelli

et al. 2014

Journal of Infectious Diseases

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Nonhuman primates are extensively used to assess strategies to prevent infection from sexual exposure to human immunodeficiency virus (HIV) and to study mechanisms of mucosal transmission. However, although semen represents one of the most important vehicles for the virus, the vast majority of preclinical challenge studies have used cell-free simian immunodeficiency virus (SIV) or simian/human immunodeficiency virus (SHIV) viral particles inoculated as diluted culture supernatants. Semen is a complex body fluid containing many factors that may facilitate or decrease HIV infectiousness. The virus in semen is present in different forms: as free virus particles or as cell-associated virus (ie, within infected leukocytes). Although cell-to-cell transmission of HIV is highly efficient, the role of cell-associated virus in semen has been surprisingly poorly investigated in nonhuman primate models. Mucosal exposure of macaques to cell-associated SIV by using infected peripheral blood mononuclear cells or spleen cells has been shown to be an efficient means of infection; however, it has yet to be shown that SIV- or SHIV-infected seminal leukocytes can transmit infection in vivo. Improvement of animal models to better recapitulate the complex microenvironment at portals of HIV entry is needed for testing candidate antiretrovirals, microbicides, and vaccines.

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Section: Leukocytes In the Semen Of Siv-infected Macaquessupporting

confidence: 83%

Nonhuman Primate Models for Cell-Associated Simian Immunodeficiency Virus Transmission: The Need to Better Understand the Complexity of HIV Mucosal Transmission

Bernard-Stoecklin

Gommet

Cavarelli

et al. 2014

Journal of Infectious Diseases

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“…We demonstrated that the biological role of leukocytes in semen is not only antimicrobial;282930 rather, leukocytes have other important biological actions. For example, another significant effect of their presence in the semen is the elimination of immature germ cells via an endogenous anti-inflammatory response 313233.…”

Section: Discussionmentioning

confidence: 85%

Male accessory gland inflammation prevalence in type 2 diabetic patients with symptoms possibly reflecting autonomic neuropathy

et al. 2014

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Male accessory gland inflammation or infection (MAGI) is a potentially underdiagnosed complication of type 2 diabetes (DM2); specifically, we reported in a recent study that the frequency of MAGI was 43% among DM2 patients. In previous studies, we have demonstrated that diabetic autonomic neuropathy (DAN) is associated with a peculiar ultrasound characterization of the seminal vesicles (SVs) in DM2 patients. The aim of the present study was to evaluate the frequency of MAGI in two different categories of DM2 patients (i.e. patients with and without symptoms that possibly reflect DAN) and the respective ultrasound characterizations. Sixty DM2 patients with a mean (± s.e.m.) age of 42.0 ± 6.0 years (range: 34–47 years) were classified according to the presence or the absence of symptoms that could possibly reflect DAN (group A: DM2 with symptoms possibly reflecting DAN, n = 28 patients and group B: DM2 without symptoms possibly reflecting DAN, n = 32 patients). The patients in Group A exhibited a significantly higher frequency of MAGI compared with those in group B patients (P < 0.05); moreover, the Group A patients exhibited a significantly higher frequency of ultrasound signs suggestive of vesiculitis (P < 0.05). Finally, the concentrations of lymphocytes but not the concentrations of the leukocytes in the semen were significantly higher (P < 0.05) in group A compared with group B.

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“…However, this method is only able to detect granulocyte (16). Conversely, flow cytometry allows the identification of all leukocyte sub-populations present in the seminal fluid (17,18). Inparticular, granulocytes represent the predominant component (from 50% to 60%), followed by macrophages (from 20% to 30%), T lymphocytes (from 2% to 5%) (16) and B lymphocytes (18).…”

Section: Discussionmentioning

confidence: 99%

Increased Lymphocyte Concentration in the Semen of Patients with Reduced Testicular Volume

et al. 2013

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Low testicular volume (TV) is associated with a decreased testicular function. Several studies explored the conventional sperm parameters and the endocrine function in patients with low TV. No other parameters have been examined. On the basis of these premises, the aim of this study was to evaluate a non-conventional seminal parameter: seminal lymphocyte characterisation in men with low TV compared with that of subjects with normal TV. A further comparison was made between fertile men and infertile patients with low or normal TV (78 patients). The testis was considered normal in size when it had a volume between 15 and 25 crrr', low-normal with a volume between 10 and 12 em" and hypotrophic when the volume was <10 cnr'. Statistically significant differences were observed in the following sperm parameters: percentage of immature germ elements, peroxidase-positive leukocyte concentration and CD45pos leukocytes (p<0.05). A correlation analysis showed the presence of a positive linear relationship between CD45pos leukocytes and the percentage of immature germ elements (r=0.88; p<0.05) and between CD45pos leukocytes and the percentage of spermatozoa with phosphatidylserine externalisation (r=0.90; p<0.05) as well as a negative linear relationship between the percentage of spermatozoa with normal morphology and the seminal CD45pos leukocyte concentration (r=-0.75; p<0.05). The results of this study showed that patients with low testicular volume «10 em') have a significantly increased CD45pos concentration that is associated with increased percentages of immature germ elements, spermatozoa with signs of early apoptosis, and spermatozoa with abnormal morphology.The testis is an oval-shaped organ that, in adult men, weighs approximately 20 g, has an average volume (TV) of 18.6±4.8 em' and dimensions ranging from 3.6 to 5.5 em in length and 2.1 to 3.5 ern in width. Within the testis, there are approximately 400-600 seminiferous tubules that are 70-80 em long and have a diameter of 0.12-0.13 mm, representing 80-90% of the total testicular volume (1). The testis consists of two structurally distinct compartments: the interstitial cell or Leydig cell compartment and the seminiferous tubule compartment, which contains germ cells and Sertoli cells. These two compartments are responsible for the two main testicular actions: the endocrine and the exocrine function (production of androgens and spermatozoa). In the clinical practice, TV can be estimated by a physical examination using an orchidometer, but it can be more objectively assessed by an ultrasound examination, which is less affected by factors such as scrotal effusions, varicose veins, intra-scrotal masses, testicular pain, and distinction with the adjacent anatomical structures (2). It has

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Isolation and characterization of T cells from semen

Cited by 12 publications

References 29 publications

Nonhuman Primate Models for Cell-Associated Simian Immunodeficiency Virus Transmission: The Need to Better Understand the Complexity of HIV Mucosal Transmission

Nonhuman Primate Models for Cell-Associated Simian Immunodeficiency Virus Transmission: The Need to Better Understand the Complexity of HIV Mucosal Transmission

Male accessory gland inflammation prevalence in type 2 diabetic patients with symptoms possibly reflecting autonomic neuropathy

Increased Lymphocyte Concentration in the Semen of Patients with Reduced Testicular Volume

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