Isolation and characterization of ten microsatellite loci for the Chong’an moustache toad Leptobrachium liui (Anura: Megophryidae)

Hu, Lujun; Xia, Yun; Zeng, Xiaomao

doi:10.1007/s12686-012-9663-0

Cited by 4 publications

(4 citation statements)

References 12 publications

(11 reference statements)

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“…The microsatellite loci included VIB‐B4, VIB‐C10, VIB‐D5, CHA1, CHA6, CHA9, LSMT6, LSMT8, CHA4, CHA7, CHA8, CHA11, LSMT4, and LSMT9 (Bi, Deng, Crosby, & Fu, ; Hu, Xia, Zheng, & Zeng, ; Wang et al., ). The PCR fragments containing primers fluorescently labeled with FAM, TAMRA, or HEX were evaluated along with the GS500 size standard using an ABI 3730 analyzer (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Examining the interglacial high‐elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui

Zeng

2018

Ecology and Evolution

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The effects of Quaternary climatic oscillations on the distributions of organisms in different parts of the world are not equally well understood, limiting the ability to understand the determinants of biodiversity. Compared with the mountain regions in southern Europe and southwestern North America, such effects on high‐elevation species in the East Asian subtropical mountain systems located in southern and southeastern China have seldom been addressed. In this study, using Leptobrachium liui (Megophryidae), we made one of the earliest attempts to examine the interglacial high‐elevation refugia scenario in these Asian mountains. Based on our current understanding of the study system, we formulated a hypothesis that these frogs of western origin were distributed more widely and continuously during glacial phases, allowing eastward dispersal, and that they are currently isolated in interglacial refugia at higher elevations. Microsatellite data and mitochondrial and nuclear sequence data were obtained with extensive sampling followed by the synthesis of phylogeographic and population genetic analyses and modeling of the species distribution. The analyses revealed a sequential eastward divergence of microsatellite clusters and gene lineages accompanied by a decline in genetic diversity. Molecular dating estimates revealed divergence events in the Pleistocene, and a reduction in local populations was inferred to have occurred at a time comparable to the end of the last glacial. Strong genetic isolation by distance reflecting a more continuous historical distribution was detected. Furthermore, environmental niche models inferred a wide planar distribution during the last glacial maximum, providing further support for the hypothesis.

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Section: Methodsmentioning

confidence: 99%

Examining the interglacial high‐elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui

Zeng

2018

Ecology and Evolution

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“…For microsatellites amplification, we used eight loci (LSMT1, LSMT8, LSMT9, CHA1, CHA4, CHA9, VIB-C5 and VIB-D5) that could be amplified in three species (Bi et al, 2009;Wang et al, 2011;Hu et al, 2012). The PCR was conducted in 10 lL volume.…”

Section: Laboratory Protocolsmentioning

confidence: 99%

Reflection of paleoclimate oscillations and tectonic events in the phylogeography of moustache toads in southern China

Wei

et al. 2018

Journal of Zoology

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Current genetic variation patterns across different geographic areas are mainly the result of various historical processes, including paleoclimate oscillations and tectonic events. Southern China, characterized by a complex paleoclimate and topography, presents an opportunity to examine such genetic variation patterns. Herein, we selected three species of moustache toads (Leptobrachium boringii, Leptobrachium liui and Leptobrachium leishanense) distributed across southern China to elucidate historical processes that shaped their current phylogeographic patterns based on two mitochondrial genes and eight microsatellite loci. L. boringii and L. liui are estimated to have genetically diverged from the late Miocene to the Pleistocene, which were largely associated with paleoclimate oscillations and geological barriers generated by complex mountain systems in southern China. Within L. liui, a recent split was caused by the rising of the Wuyi Mountains during the early Pleistocene. Demographic analyses revealed that complex topography might play buffering roles in Leptobrachium species during the last glacial maximum. These findings not only highlight the combined effects of climatic oscillations and tectonic events on driving genetic divergences, but also contribute to the general understanding of the phylogeography in this biodiversity hotspot.

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“…In addition, dinucleotide microsatellites are considered less efficient and more unreliable than tetranucleotides because of their minimal PCR stutter [ 25 ]. Moreover, the traditional methods of microsatellite isolation and characterization are quite involved, costly, and time-consuming [ 26 ]. With the publication of a number of genomes, we can obtain sufficient numbers of different types of useful microsatellite loci more efficiently [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Dynamics of Leptobrachium leishanense (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome

Cai

et al. 2021

Animals

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Persisting declination of amphibians around the world has resulted in the public attaching importance to the conservation of their biodiversity. Genetic data can be greatly helpful in conservation planning and management, especially in species that are small in size and hard to observe. It is essential to perform genetic assessments for the conservation of Leptobrachium leishanense, an endangered toad and receiving secondary protection on the list of state-protected wildlife in China. However, current molecular markers with low reliability and efficiency hinder studies. Here, we sampled 120 adult toes from the population in the Leishan Mountain, 23 of which were used to develop tetranucleotide microsatellite markers based on one reference L. leishanense genome. After primer optimization, stability detection, and polymorphism detection, we obtained 12 satisfactory microsatellite loci. Then, we used these loci to evaluate the genetic diversity and population dynamics of the 120 individuals. Our results show that there is a low degree of inbreeding in the population, and it has a high genetic diversity. Recently, the population has not experienced population bottlenecks, and the estimated effective population size was 424.3. Accordingly, stabilizing genetic diversity will be key to population sustainability. Recovering its habitat and avoiding intentional human use will be useful for conservation of this species.

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Isolation and characterization of ten microsatellite loci for the Chong’an moustache toad Leptobrachium liui (Anura: Megophryidae)

Cited by 4 publications

References 12 publications

Examining the interglacial high‐elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui

Examining the interglacial high‐elevation refugia scenario in East Asian subtropical mountain systems with the frog species Leptobrachium liui

Reflection of paleoclimate oscillations and tectonic events in the phylogeography of moustache toads in southern China

Genetic Diversity and Population Dynamics of Leptobrachium leishanense (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome

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