Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

Yang, Lixia; Liang, Jianhua; Zhou, Weizheng; Su, Liangchen; Zhang, Biyu; Li, Ling

doi:10.1007/s11105-010-0259-0

Cited by 6 publications

(6 citation statements)

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“…Likewise, as reported in this work for soybean, higher expression of the drought-responsive endogenous genes GmAREB1, GmPP2C, GmSnRK2, and GmAAO3 was also reported in Arabidopsis genetically modified with AtNCED3 (Iuchi et al, 2001). Considering genes from the ABA pathway, an increase in aldehyde oxidase genes under WD was reported in Arabidopsis (Koiwai et al, 2004), pea (Zdunek-Zastocka andSobczak, 2013) and peanut (Yang et al, 2011). In A. thaliana, AAO3 transcripts presented a strong increase in response to water deficit (Koiwai et al, 2004).…”

Section: Discussionsupporting

confidence: 83%

“…In Arachis hypogaea L., a dominant expression of the AhAO2 gene was observed in leaves. The overexpression of AhAO2 in Arabidopsis headed to an increase in ABA levels and improved drought tolerance (Yang et al, 2011). In pea (Pisum sativum), during a progressively induced drought treatment, levels of PsAO3 transcripts increased expressively in roots and leaves, while ABA accumulation was identified only in leaves, complemented by the induction of the PsNCED3 gene expression (Zdunek-Zastocka and Sobczak, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of AtNCED3 gene improved drought tolerance in soybean in greenhouse and field conditions

et al. 2020

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Water deficit is an important climatic problem that can impair agriculture yield and economy. Genetically modified soybean plants containing the AtNCED3 gene were obtained aiming drought-tolerance improvement. The NCED3 gene encodes a 9-cis-epoxycarotenoid dioxygenase (NCED, EC 1.13.11.51), an important enzyme in abscisic acid biosynthesis. ABA activates the expression of drought-responsive genes, in water-deficit conditions, targeting defense mechanisms and enabling plants to survive under low water availability. Results from greenhouse experiments showed that the transgene AtNCED3 and the endogenous genes GmAREB1, GmPP2C, GmSnRK2 and GmAAO3 presented higher expression under water deficit (WD) in the event 2Ha11 than in WT-plants. No significant correlation was observed between the plant materials and WD conditions for growth parameters; however, gas exchange measurements decreased in the GM event, which also showed 80% higher intrinsic water use when compared to WT plants. In crop season 2015/16, event 2Ha11 showed higher total number of pods, higher number of pods with seeds and yield than WT plants. ABA concentration was also higher in GM plants under WD. These results obtained in field screenings suggest that AtNCED3 soybean plants might outperform under drought, reducing economic and yield losses, thus being a good candidate line to be incorporated in the soybean-breeding program to develop droughttolerant cultivars.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Overexpression of AtNCED3 gene improved drought tolerance in soybean in greenhouse and field conditions

et al. 2020

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“…Among four abscisic aldehyde oxidases (AtAAO1 to 4) in Arabidopsis , AtAAO3 was reported to actively utilize abscisic aldehyde as a substrate, most probably the only one AAO involved in ABA biosynthesis [11]. Here our previously characterized two peanut AAO genes, AhAAO1 [52] and AhAAO2 [53], were also screened from the constructed drought-induced transcriptome of peanut leaves. AhAAO1 protein was predicted to localize in the cytosol by the WoLF PSORT tool, and AhAAO2 was predicted by the iPSORT algorithm as not having any of signal, mitochondrial targeting, or chloroplast transit peptides.…”

Section: Resultsmentioning

confidence: 99%

An abscisic acid (ABA) homeostasis regulated by its production, catabolism and transport in peanut leaves in response to drought stress

et al. 2019

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ABA is an important messenger that acts as a signaling mediator for regulating the adaptive response of plants to drought stress. Two production pathways, de novo biosynthesis and hydrolysis of glucose-conjugated ABA by β-glucosidase (BG), increase cellular ABA levels in plants. ABA catabolism via hydroxylation by 8’-hydroxylase (CYP707A), or conjugation by uridine diphosphate glucosyltransferase (UGT), decreases cellular ABA levels. The transport of ABA through ATP-binding cassette (ABC)-containing transporter proteins, members of ABC transporter G family (ABCG), across plasma membrane (PM) is another important pathway to regulate cellular ABA levels. In this study, based on our previously constructed transcriptome of peanut leaves in response to drought stress, fourteen candidate genes involved in ABA production (including AhZEP , AhNCED1 and AhNCED3 , AhABA2 , AhAAO1 and AhAAO2 , AhABA3 , AhBG11 and AhBG24 ), catabolism (including AhCYP707A3 , AhUGT71K1 and AhUGT73B4 ) and transport (including AhABCG22-1 and AhABCG22-2 ), were identified homologously and phylogenetically, and further analyzed at the transcriptional level by real-time RT-PCR, simultaneously determining ABA levels in peanut leaves in response to drought. The high sequence identity and very similar subcellular localization of the proteins deduced from 14 identified genes involved in ABA production, catabolism and transport with the reported corresponding enzymes in databases suggest their similar roles in regulating cellular ABA levels. The expression analysis showed that the transcripts of AhZEP , AhNCED1 , AhAAO2 and AhABA3 instead of AhABA2 , AhNCED3 and AhAAO1 in peanut leaves increased significantly in response to drought stress; and that the AhBG11 and AhBG24 mRNA levels were rapidly and significantly up-regulated, with a 4.83- and 4.58-fold increase, respectively at 2-h of drought stress. The genes involved in ABA catabolism AhCYP707A3 , AhUGT71K1 instead of AhUGT73B4 were significantly induced in response to drought stress. The expression of two closely related peanut ABCG genes, AhABCG22 . 1 and AhABCG22 . 2 , was significantly up-regulated in response to drought stress. The ABA levels rapidly began to accumulate within 2 h (a 56.6-fold increase) from the start of drought stress, and peaked at 10 h of the stre...

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“…Among four abscisic aldehyde oxidases (AtAAO1 to 4) in Arabidopsis , AtAAO3 was reported to actively utilize abscisic aldehyde as a substrate, most probably the only one AAO involved in ABA biosynthesis [11]. Here our previously characterized two peanut AAO genes, AhAAO1 [51] and AhAAO2 [52], were also screened from the constructed drought-induced transcriptome of peanut leaves. AhAAO1 protein was predicted to localize in the cytosol by the WoLF PSORT tool, and AhAAO2 was predicted by the iPSORT algorithm as not having any of signal, mitochondrial targeting, or chloroplast transit peptides.…”

Section: Resultsmentioning

confidence: 99%

An abscisic acid (ABA) homeostasis regulated by its production, catabolism and transport in peanut leaves in response to drought stress

Long

Zheng

Zhang

et al. 2019

Preprint

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26ABA is an important messenger that acts as the signaling mediator for regulating the adaptive response of 27 plants to drought stress. Two production pathways, de novo biosynthesis and hydrolysis of 28 glucose-conjugated ABA by β-glucosidase (BG), increase cellular ABA levels in plants. ABA catabolism 29 via hydroxylation by 8'-hydroxylase (CYP707A), or conjugation by uridine diphosphate 30 glucosyltransferase (UGT), decreases cellular ABA levels. The transport of ABA through ATP-binding 31 cassette (ABC)-containing transporter proteins, members of ABC transporter G family (ABCG), across 32 plasma membrane (PM) is another important pathway to regulate cellular ABA levels. In this study, 33 based on our previously constructed transcriptome of peanut leaves in response to drought stress, fourteen 34 candidate genes involved in ABA production (including AhZEP, AhNCED1 and AhNCED3, AhABA2, 35 AhAAO1 and AhAAO2, AhABA3, AhBG11 and AhBG24), catabolism (including AhCYP707A3, 36 AhUGT71K1 and AhUGT73B4) and transport (including AhABCG22-1 and AhABCG22-2), were 37 identified homologously and phylogenetically, and further analyzed at the transcriptional level by 38 real-time RT-PCR, simultaneously determining ABA levels in peanut leaves in response to drought. The 39 high sequence identity and very similar subcellular localization of the proteins deduced from 14 identified 40 genes involved in ABA production, catabolism and transport with the reported corresponding enzymes in 41 databases suggest their similar roles in regulating cellular ABA levels. In response to drought stress, ABA 42 accumulation levels in peanut leaves agree very well with the up-regulated expressions of 43 ABA-producing genes (AhZEP, AhNCED1, AhAAO2, AhABA3, AhBG11 and AhBG24) and PM-localized 44 ABA importer genes (AhABCG22-1 and AhABCG22-2), although the expression of ABA catabolic genes 45 (AhCYP707A3 and AhUGT71K1) was also up-regulated. It is likely that drought-responsive induction of 46 catabolic genes helps not only to maintain ABA levels within a permissible range, but also to prepare the 3 47 plant for degradation of ABA after removal of the stress. These results suggest that ABA homeostasis in 48 peanut leaves in response to drought may be coordinated by a master regulatory circuit that involves 49 production, catabolism, and as well as transport. 50 73 stomatal closure, seed dormancy, growth and various abiotic stress responses [1,2]. ABA is mainly 74 produced by the de novo biosynthetic pathway through the oxidative cleavage of carotenoids [3]. In this 75 pathway, zeaxanthin epoxidase (ZEP/ABA1) catalyzes the formation of all transviolaxthin from 76 zeaxanthin [4]. Nine cis-epoxycarotenoid dioxygenase (NCED) cleaves carotenoids to form xanthoxin 77 [5,6]. Xanthoxin is assumed to be transported from the plastids to the cytosol, although the precise 78 mechanism that mediates this transport is not yet known [2]. The short-chain alcohol 79 dehydrogenase/reductase (SDR/ABA2) converts xanthoxin derived from cleavage of carot...

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Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

Cited by 6 publications

References 46 publications

Overexpression of AtNCED3 gene improved drought tolerance in soybean in greenhouse and field conditions

Overexpression of AtNCED3 gene improved drought tolerance in soybean in greenhouse and field conditions

An abscisic acid (ABA) homeostasis regulated by its production, catabolism and transport in peanut leaves in response to drought stress

An abscisic acid (ABA) homeostasis regulated by its production, catabolism and transport in peanut leaves in response to drought stress

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