“…A variety of techniques have been employed in attempts to isolate piscine Ig with varying results (Acton 1971, Bradshaw et al 1971, Clerx et al 1980, Lobb 1981a, b, Ourth 1986, Phillips & Ourth 1986, Havarstein et al 1988, Smith et al 1993a). Smith et al (1993a) injected fish with goat IgG without a n adjuvant to produce polyclonal anti-goat Ig in the fish.…”