Isolation and characterization of the novel bacteriophage vB_SmaS_BUCT626 against Stenotrophomonas maltophilia

Characterization and genome analysis of phage vB_KpnS_SXFY507 against Klebsiella pneumoniae and efficacy assessment in Galleria mellonella larvae

“…The untreated sewage sample was collected from a public hospital in Taiyuan, China. Phage isolation and purification were performed as previously described ( Feng et al, 2021 ; Li F. et al, 2022 ). Briefly, the sewage sample was centrifuged, and then the supernatant was filtered through a sterile 0.22 μm filter (Millipore, PES membrane).…”

“…The multiplicity of infection (MOI) determination was carried out as previously described ( Li F. et al, 2022 ). Phage vB_KpnS_SXFY507 was incubated with the log-phase culture of K. pneumoniae SXFY507 at various proportions (10, 1, 0.1, 0.01, and 0.001) followed by incubation at 37°C 160 rpm for 5 h. The mixture was centrifuged to remove bacterial cells, and the supernatant was filtered by a 0.22 μm filter.…”

“…The one-step growth curve was determined to measure the incubation period and the burst size of the phage ( Li F. et al, 2022 ). Phage vB_KpnS_SXFY507 was incubated with K. pneumoniae SXFY507 at an MOI of 0.001.…”

“…The host range of phage vB_KpnS_SXFY507 was determined by a spot test using 27 K. pneumoniae strains (25 clinical strains, ATCC BAA-2146, and, ATCC BAA-1705; Li F. et al, 2022 ). Briefly, 100 μl of log-phase cultures of each strain was mixed with 5 ml of top agar (BHI with 0.75% agar), and then poured onto individual plates containing solid medium to form a double-layered plate.…”

“…The genomic DNA of phage vB_KpnS_SXFY507 was extracted using the protease K/SDS method as previously described ( Li F. et al, 2022 ). The NEBNext Ultra II FS DNA Library Prep Kit (NEB) for Illumina was used for library preparation.…”