Isolation and characterization of the exopolysaccharide produced by Streptococcus thermophilus SFi20

Navarini, Luciano; Abatangelo, Anna; Bertocchi, Claudia; Conti, Elena; Bosco, Marcella; Picotti, Fabrizio

doi:10.1016/s0141-8130(01)00118-0

Cited by 42 publications

(27 citation statements)

References 26 publications

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“…The repeating units most often contain a combination of D-glucose, D-galactose, and L-rhamnose, albeit in different ratios. In a few cases, L-fucose, acetylated amino sugars (e.g., N-acetyl-D-galactosamine), D-ribose, D-glucuronic acid, and D-nononic acid, as well as noncarbohydrate constituents such as glycerol, phosphate, pyruvyl, and acetyl groups, are present (15,21,26,35,41,50,51,68,69).…”

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confidence: 99%

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Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Vaningelgem

Zamfir

Mozzi

et al. 2004

Appl Environ Microbiol

193

163

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Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared with their milk clotting behaviors. The majority of the strains grew better when yeast extract and peptone were added to the milk medium, although the presence of interfering glucomannans was shown, making this medium unsuitable for EPS screening. EPS production was found to be strain dependent, with the majority of the strains producing between 20 and 100 mg of polymer dry mass per liter of fermented milk medium. Furthermore, no straightforward relationship between the apparent viscosity and EPS production could be detected in fermented milk medium. An analysis of the molecular masses of the isolated EPS by gel permeation chromatography revealed a large variety, ranging from 10 to >2,000 kDa. A distinction could be made between highmolecular-mass EPS (>1,000 kDa) and low-molecular-mass EPS (<1,000 kDa). Based on the molecular size of the EPS, three groups of EPS-producing strains were distinguished. Monomer analysis of the EPS by highperformance anion-exchange chromatography with amperometric detection was demonstrated to be a fast and simple method. All of the EPS from the S. thermophilus strains tested were classified into six groups according to their monomer compositions. Apart from galactose and glucose, other monomers, such as (N-acetyl)galactosamine, (N-acetyl)glucosamine, and rhamnose, were also found as repeating unit constituents. Three strains were found to produce EPS containing (N-acetyl)glucosamine, which to our knowledge was never found before in an EPS from S. thermophilus. Furthermore, within each group, differences in monomer ratios were observed, indicating possible novel EPS structures. Finally, large differences between the consistencies of EPS solutions from five different strains were assigned to differences in their molecular masses and structures.

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confidence: 99%

“…The molecular masses of the EPS from LAB range from 10 kDa (71) and over 200 kDa (1) to Ͼ1,000 kDa (9,26,32,41). Importantly, both structure and molecular mass influence the rheological properties of a polysaccharide (18,61).…”

mentioning

confidence: 99%

Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Vaningelgem

Zamfir

Mozzi

et al. 2004

Appl Environ Microbiol

193

163

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“…In this regard, spin diffusion technique, a complement to the hetero nuclear multiple bond correlation, has provided positive results on the determination of linkage pattern of exopolysaccharide monomers (Vincent and Zwahlen, 2000). Recently suggestions have been made on the use of deuterium-induced differential isotope shifts to analyze the linkage pattern of exopolysaccharides monomers which can be a good substitute to twodimensional nuclear magnetic resonance technique and shaped pulses since these techniques result in the deletion of methylation step, leading to direct inspection of nuclear magnetic resonance of polysaccharide complex molecules (Navarini et al, 2001;Bendiak, 2002). In general, analysis and differentiation of different exopolysaccharides in various taxa of different groups of lactic acid bacteria by traditional and conventional methods assess the number of monosaccharides present in the repeating backbone units of oligosaccharide molecules which reflect the sugar residual numbers in the main oligosaccharide chain.…”

Section: Molecular Characterization Of Exopolysaccharidementioning

confidence: 99%

Exopolysaccharide and lactic acid bacteria: Perception, functionality and prospects

Bajpai

Rather

Majumder

et al. 2015

Bangladesh J Pharmacol

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Lactic acid bacteria exhibit the most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits. This review provides an update on multiple uses and production of exopolysaccharides with major emphasis on their chemical properties, characterization, and some other molecular strategies adopted for their genetics and biological tailoring to better understand the process of exopolysaccharide production along with their antiviral efficacy with multiple modes of action. Additionally, microbiological, biochemical, nutritional and biotechnological aspects of exopolysaccharide production have also been discussed. Moreover, appro-priate suggestions have been made on lactic acid bacteria improvements, leading to enhanced production with advanced modification and production process that may contribute to the economic soundness of applications in food and pharmacological industries with this promising group of biomolecules.

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“…Furthermore, N-acetylgalactosamine, fucose, and ribose are also components of EPS (Figure 3) [39][40][41][42][43][44][45][46][47][48][49]. Galactose includes not only galactopyranose, but also galactofuranose.…”

Section: Ctures Of Eps In S Thermophilusmentioning

confidence: 99%

Biofilm Formation by Streptococcus mutans and Related Bacteria

Nishimura¹,

Saito²,

Yoneyama³

et al. 2012

AiM

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Isolation and characterization of the exopolysaccharide produced by Streptococcus thermophilus SFi20

Cited by 42 publications

References 26 publications

Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Exopolysaccharide and lactic acid bacteria: Perception, functionality and prospects

Biofilm Formation by <i>Streptococcus mutans</i> and Related Bacteria

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Isolation and characterization of the exopolysaccharide produced by Streptococcus thermophilus SFi20

Cited by 42 publications

References 26 publications

Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Biodiversity of Exopolysaccharides Produced by Streptococcus thermophilus Strains Is Reflected in Their Production and Their Molecular and Functional Characteristics

Exopolysaccharide and lactic acid bacteria: Perception, functionality and prospects

Biofilm Formation by &lt;i&gt;Streptococcus mutans&lt;/i&gt; and Related Bacteria

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Biofilm Formation by <i>Streptococcus mutans</i> and Related Bacteria