Isolation and Characterization of the Bovine Microsatellite Loci

Abstract

Microsatellite loci were isolated using five repetitive probes for Korean native cattle. Eleven microsatellite loci were developed based on a biotin hybrid capture method, and enrichment of the genomic libraries (AAAT, TG, AG, T, and TGC repeats) was performed using Sau3AI adapters. The isolated markers were tested in two half-sib Korean cattle families and four imported breeds (Angus, Limousine, Holstein, and Shorthorn). Nine informative microsatellite loci were observed, and two microsatellite loci were revealed as monomorphic in Korean cattle. In the imported breeds, however, all of the markers were informative. In total, 213 alleles were obtained at the 11 loci across five breeds, and the average number of alleles found per locus, considering all populations, was 4.26. Heterozygosity was 0.71 (expected) and 0.57 (observed). The range of the polymorphic information content for the markers in all cattle populations was 0.43-0.69. Eleven percent of genetic variation was attributed to differentiation between populations as determined by the mean F (ST) values. The remaining 89% corresponded to differences among individuals. The isolated markers may be used to identify and classify the local breeds on a molecular basis.

Forensic Characterization of Four New Bovine Tri-nucleotide Microsatellite Markers in Korean Cattle (Hanwoo)

“…P-values at each locus were 0.673 (B17S0808), 0.153 (B15S6253), 0.104 (B8S7996), and 0.405 (B17S4998), all of which were in Hardy-Weinberg equilibrium (P > 0.05). Many other bovine microsatellite markers including ISAG-recommended markers showed significant deviations from Hardy-Weinberg equilibrium in southwestern European breeds (Beja-Pereira et al, 2003), Brazilian Nellore cattle (Cerviniet al, 2006), and many Asian cattle breeds including Korean native breed (Chung et al, 2006;Sun et al, 2008). Deviations from Hardy-Weinberg equilibrium could change allele frequencies of the loci from generation to generation.…”