Isolation  and  characterization  of  the  &lt;em&gt;Staphylococcus  aureus&lt;/em&gt; bacteriophage vB_SauS_SA2

Wang, Jia; Zhao, Feiyang; Sun, Huzhi; Wang, Qian; Zhang, Can; Liu, Wenhua; Zou, Ling; Pan, Qinxue; Ren, Huiying

doi:10.3934/microbiol.2019.3.285

Cited by 26 publications

(19 citation statements)

References 49 publications

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“…It can withstand the raised temperature till 45ºC but became inactivated at 65 ºC. These results were similar to phages SA2 and SLPW where high temperature progressively inactivated their activity [32]. It exhibit good pH stability at wide range of pH (5-9), and optimum activity at neutral pH.…”

Section: Discussionsupporting

confidence: 63%

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

Tabassum¹,

Alvi²,

Asif³

et al. 2021

Preprint

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Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing nosocomial pathogen that causes a large number of diseases in healthcare and community settings. The MRSA causes infections in different tissues of immunocompromised individuals leading to increased morbidity and mortality. It possess various virulence mechanisms to show resistance against to a lot of beta-lactam antibiotics. To tackle this emerging issue of MRSA, there is an urgent need of antibiotic alternatives and utilizing lytic bacteriophages is one of the best promising therapeutic approach. In the present study, a lytic bacteriophage TSP was isolated from hospital wastewater against MRSA. Its morphology, physiology, host specificity, burst size and lytic spectrum were determined and complete genome sequence was analyzed. TSP phage efficiently inhibit bacterial growth for up to 12 hours. TSP phage showed broad lytic spectrum against clinical isolates of MRSA (78%) and MSSA (37%). It showed stability at varying temperatures (25ºC, 37ºC) and pH (5–9), while its maximum storage stability was observed at 4ºC. It had short latent period (20min) and high burst size (103 PFU/ infected cell). TSP genome sequence and restriction analysis revealed that its genome is linear having 17,987 bp in length with an average GC content of 29.7%. The TSP genome showed 98% similarity to S aureus phages SCH1, SCH11 and vB SauP-436A1. According to comparative genomic analysis and phylogenetic tree analysis, TSP phage can be considered as a member of genus “P68viruses”. The strong lytic activity, broad host range and short latent period along with absence of any lysogenic and toxic genes make TSP a very good candidate for phage therapy against MRSA infections if prove safe during in vivo studies.

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Section: Discussionsupporting

confidence: 63%

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

Tabassum¹,

Alvi²,

Asif³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…TSP showed longer survival ability and performance at physiological temperature (37 °C), which favors its therapeutic application against MRSA infections. However, it became inactive at 65 °C, which agreed with decrease in stability of vB_SauS_SA2 and SLPW phages at elevated temperature 42 . TSP exhibits stability over a wide range of pHvalues (5–9) and optimum activity at neutral pH, which agreed with previously reported studies 7 , 27 , 43 .…”

Section: Discussionsupporting

confidence: 59%

TSP, a virulent Podovirus, can control the growth of Staphylococcus aureus for 12 h

Tabassum

Basit

Alvi

et al. 2022

Sci Rep

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Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing nosocomial pathogen that is increasingly isolated in community settings. It shows resistance against all beta-lactam drugs and has acquired mechanisms to resist other groups of antibiotics. To tackle this emerging issue of MRSA, there is an urgent need for antibiotic alternatives, and utilizing lytic bacteriophages is one of the most promising therapeutic approaches. In the present study, a lytic bacteriophage TSP was isolated from hospital wastewater against MRSA. The phage efficiently inhibited bacterial growth for up to 12 h at MOI of 1 and 10. TSP phage showed activity against various isolates of MRSA and MSSA, isolated from different clinical samples, with variable antibiotic susceptibility patterns. The bacteriophage TSP showed stability at varying temperatures (25 °C, 37 °C) and pH values (5–9), while its maximum storage stability was observed at 4 °C. It had a short latent period (20 min) and burst size of 103 ± 5pfu/infected cells. TSP genome sequence and restriction analysis revealed that its genome has a linear confirmation and length of 17,987 bp with an average GC content of 29.7%. According to comparative genomic analysis and phylogenetic tree,TSP phage can be considered a member of genus “P68viruses”. The strong lytic activity and short latent period in addition to its lytic nature makes it a good candidate for phage therapy against MRSA infections, if it proves to be effective in in-vivo studies.

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“…Bacterial killing assays were conducted for the phages as previously described [69]. Briefly, A. hydrophila NJ-35 and XY-16 suspensions ( CFU/mL) in exponential period were mixed with an equal volume of phages at different MOIs of 0.01, 0.1, 1, and 10, respectively, followed by incubation at 28°C for 24 h. A. hydrophila cultures mixed with LB broth served as positive controls.…”

Section: Phage Killing Assay In Vitromentioning

confidence: 99%

Isolation and characterization of bacteriaophages against virulent Aeromonas hydrophila

Jin

Gao

Dong

et al. 2020

Preprint

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Background: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. Results: Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. Conclusions: Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy.

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Isolation and characterization of the <em>Staphylococcus aureus</em> bacteriophage vB_SauS_SA2

Cited by 26 publications

References 49 publications

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

TSP, a virulent Podovirus, can control the growth of Staphylococcus aureus for 12 h

Isolation and characterization of bacteriaophages against virulent Aeromonas hydrophila

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