Isolation and characterization of the<i>Serratia entomophila</i>antifeeding prophage

Hurst, Mark R. H.; Beard, Sam S.; Jackson, Trevor A.; Jones, Scott

doi:10.1111/j.1574-6968.2007.00645.x

Cited by 78 publications

(82 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sixteen of these genes encode proteins that could form a defective prophage (called Afp for antifeeding prophage) 42 . Expression of the afp genes in E. coli allows the synthesis of particles that resemble phage tail-like bacteriocins 43 . These particles might function as a delivery device for the putative toxins (or effectors) that are encoded by the last two genes.…”

Section: Pathogenicmentioning

confidence: 99%

Bacterial strategies to overcome insect defences

2008

View full text Add to dashboard Cite

| Recent genetic and molecular analyses have revealed how several strategies enable bacteria to persist and overcome insect immune defences. Genetic and genomic tools that can be used with Drosophila melanogaster have enabled the characterization of the pathways that are used by insects to detect bacterial invaders and combat infection. Conservation of bacterial virulence factors and insect immune repertoires indicates that there are common strategies of host invasion and pathogen eradication. Long-term interactions of bacteria with insects might ensure efficient dissemination of pathogens to other hosts, including humans. R E V I E W S302 | APRIL 2008 | voLUME 6 www.nature.com/reviews/micro

show abstract

Section: Pathogenicmentioning

confidence: 99%

Bacterial strategies to overcome insect defences

2008

View full text Add to dashboard Cite

show abstract

“…Amber disease caused by S. entomophila A1MO2 is a multifactorial event, and multiple virulence factors might be involved; therefore, multiple virulence factors are hypothesized for S. entomophila Mor4.1. A recent study of S. entomophila A1MO2 has shown the induction of a phage tail-like bacteriocin that causes AFE, amber coloration, and mortality of C. zealandica larvae (15). The production and active role in disease of similar structures or the presence of homologues to the sepABC genes or homologues to the insecticidal toxin complex genes by S. entomophila Mor4.1 is not discarded, but the fact that S. entomophila Mor4.1 does not cause amber coloration in bioassays, which seems to be associated with the SepABC toxins (19), suggests also that different virulence factors might be implicated in S. entomophila Mor4.1 pathogenicity.…”

Section: Vol 74 2008 S Entomophila Strain Pathogenic To Scarabaeidmentioning

confidence: 99%

“…Products of the sepABC genes are considered homologues of the insecticidal toxin complex (Tc) (18) produced by the nematode-associated bacterium Photorhabdus luminescens (4). It is thought that the defective prophage might form a virus-like structure able to cause the AFE and mortality in C. zealandica larvae (15).…”

mentioning

confidence: 99%

Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

Nuñez-Valdez

Calderón

Aranda

et al. 2008

Appl Environ Microbiol

View full text Add to dashboard Cite

The larvae of scarab beetles, known as "white grubs" and belonging to the genera Phyllophaga and Anomala (Coleoptera: Scarabaeidae), are regarded as soil-dwelling pests in Mexico. During a survey conducted to find pathogenic bacteria with the potential to control scarab larvae, a native Serratia sp. (strain Mor4.1) was isolated from a dead third-instar Phyllophaga blanchardi larva collected from a cornfield in Tres Marías, Morelos, Mexico. Oral bioassays using healthy P. blanchardi larvae fed with the Mor4.1 isolate showed that this strain was able to cause an antifeeding effect and a significant loss of weight. Mortality was observed for P. blanchardi, P. trichodes, and P. obsoleta in a multidose experiment. The Mor4.1 isolate also caused 100% mortality 24 h after intracoelomic inoculation of the larvae of P. blanchardi, P. ravida, Anomala donovani and the lepidopteran insect Manduca sexta. Oral and injection bioassays were performed with concentrated culture broths of the Mor4.1 isolate to search for disease symptoms and mortality caused by extracellular proteins. The results have shown that Mor4.1 broths produce significant antifeeding effects and mortality. Mor4.1 broths treated with proteinase K lost the ability to cause disease symptoms and mortality, in both the oral and the injection bioassays, suggesting the involvement of toxic proteins in the disease. The Mor4.1 isolate was identified as a putative Serratia entomophila Mor4.1 strain based on numerical taxonomy and phylogenetic analyses done with the 16S rRNA gene sequence. The potential of S. entomophila Mor4.1 and its toxins to be used in an integrated pest management program is discussed.

show abstract

“…The Afp particle has a central tube surrounded by a contractile sheath and a baseplate with associated fibers (6). Similar particles with toxin-delivery functions are encoded by the Photorhabdus virulence cassettes (4, 7), which in turn resemble several R-type pyocins (8,9).…”

mentioning

confidence: 99%

Three-dimensional Structure of the Toxin-delivery Particle Antifeeding Prophage of Serratia entomophila

Heymann

Bartho

Rybakova

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Antifeeding prophage (Afp) is a toxin-delivery bacteriophage tail-like particle. Results: The syringe-like three-dimensional structure, composed of a helical sheath formed by 10 disks, a baseplate, and a central tube displays 6-fold symmetry. Conclusion: Although similar to other type VI secretion systems, Afp possesses unique features. Significance: This is the first insight into the three-dimensional structure of a tailocin.

show abstract

Isolation and characterization of theSerratia entomophilaantifeeding prophage

Cited by 78 publications

References 27 publications

Bacterial strategies to overcome insect defences

Bacterial strategies to overcome insect defences

Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

Three-dimensional Structure of the Toxin-delivery Particle Antifeeding Prophage of Serratia entomophila

Contact Info

Product

Resources

About