Isolation and characterization of the porcine c-myc proto-oncogene and chromosomal assignment to SSC 4p13

Reiner, Gerald; Hecht, Werner; Leeb, Tosso; Brenig, Bertram; Robic, Annie; Dzapo, V.

doi:10.1046/j.1365-2052.1999.00447.x

Cited by 7 publications

(8 citation statements)

References 14 publications

(13 reference statements)

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“…They found out a higher level of carcass value in animals with AA genotype in C-MYC gene in Polish pig breeds. Reiner et al (1999) stated that there was a direct relation between C-MYC genotypes and carcass value. In comparison with these results Resová (2001), who studied associations between C-MYC genes of pigs, found out that pigs with AB Tables 5 and 6.…”

Section: Resultsmentioning

confidence: 99%

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The analysis of carcass value in pigs of different genotypes

Čechová¹,

Mikule²

2004

CZECH J ANIM SCI

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The ability of exact determination of genotype of individual pigs in a chosen locus brings about a possibility of studying possible associations between determined genotype and expected production traits. Different stations in the world deal with this issue. In many cases genetic researches are aimed at detection of loci (position, a part of chromosome) that have a statistically significant effect on some of the parameters of efficiency (Dvořák, 1999). The results of their research are mentioned by Kahánková et al. (1996), Fiedler et al. (1999), Cieslak et al. (2000.In addition to relations to reproductive traits a lot of authors specialize in analyses of results of carcass value traits. Aspiration of scientific workstations is an exploitation of determined results in subsequent selection work in individual pig populations.Besides the most frequently studied gene RYR1 there is an effort to use other genes for determination of possible relation with carcass value of pigs. One of them is MYF4 gene, a member of MyoD family, which is responsible for differentiation of proliferous myoblasts and its genetic variability (which is probably related with the number of myofibrils) ranks it among candidate genes. Pietruzska et al. (2002) studied the relation of myogenin to meatiness. In a set of 118 tested pigs they identified 98 animals with AA genotype and 20 pigs with AB genotype. In the set no animal with BB genotype was detected. Meatiness of slaughter pigs with AA genotype reached 53% and it was by 2.3% higher in comparison with AB heterozygotes. Kaniak and Jasek (1999) reported significantly higher meatiness of AA homozygotes in comparison with AB heterozygotes and slight dominance of AA homozygotes over pigs with BB genotype. Kahánková and Dvořák (1998) stated that animals with recessive allele B showed lower meatiness in comparison with AA homozygotes. Kolaříková et al. (2002) studied the influence of myogenin gene polymorphism on meat efficiency in Czech Large White pigs. They found out no significant differences in muscle depth in pigs with BB genotype in comparison with pigs with AA and AB genotype. They did not determine any relation with backfat thickness. In this breed statistically significant differences (P ≤ 0.05) were detected between the genotypes AA and AB in these traits: MLLT area, percentage of main lean cuts and weight of main lean cuts in favour of genotype BB of MYF4 gene. We did not find any significant differences in the measured levels of carcass value traits between different genotypes of MYF4 gene in L breed.

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Section: Resultsmentioning

confidence: 99%

“…In Polish pig breeds they found a higher level of chosen carcass traits in pigs with genotype AA. Reiner et al (1999) found a direct relation between C-MYC genotypes and carcass value.…”

mentioning

confidence: 94%

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The analysis of carcass value in pigs of different genotypes

Čechová¹,

Mikule²

2004

CZECH J ANIM SCI

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“…These technologies include the identi®cation and isolation of speci®c cells and cell products which play a major role in living organisms (hormones, growth factors, immune products, cellular toxins) , Reiner et al 1999, Grigorescu et al 2000, Nurdin et al 2000, with a particular therapy of encapsulation, involving the immunoprotection of mammalian cells (Rowley et al 1999). Current pre-clinical applications include the bioarti®cial liver for hepatic failure (Roger et al 1998) and diabetes therapy (Shapiro et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…A number of new technologies have been developed, and re®ned, over the past two decades to provide a means for cell transplantation in the treatment of human diseases , Reiner et al 1999, Rowley et al 1999, Grigorescu et al 2000, Nurdin et al 2000. These technologies include the identi®cation and isolation of speci®c cells and cell products which play a major role in living organisms (hormones, growth factors, immune products, cellular toxins) , Reiner et al 1999, Grigorescu et al 2000, Nurdin et al 2000, with a particular therapy of encapsulation, involving the immunoprotection of mammalian cells (Rowley et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Polyvinylamine hydrochloride-based microcapsules: polymer synthesis, permeability and mechanical properties

Grigorescu

Rehor

Hunkeler

2002

Journal of Microencapsulation

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Mechanically stable microcapsules, with sizes of 0.4-1.5 mm, have been produced with permeabilities appropriate for applications involving living cells and controlled delivery. Polyvinylamine hydrochloride was employed alone, in miXtures with poly(methylene-co-guanidine) hydrochloride, or as a coating material for pre-formed capsules. The influence of polymer molar mass, the ratio between the two polycations, the coating time, and the capsule size on the properties of the capsules were analysed. The competitive displacement of one polycation with another in the polysaccharide matrix was also documented. The properties of the capsules vary remarkably, depending of the polyelectrolyte combinations used for their preparation. Specifically, capsules could withstand compressive loads of between 0.09-1.67 N, while the permeability varied from 10-120 kDa. Both are within the ranges required for clinical immunosuppressive therapies.

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Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease

et al. 2013

Transgenic Res

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After several decades of research, autosomal dominant polycystic kidney disease (ADPKD) is still incurable and imposes enormous physical, psychological, and economic burdens on patients and their families. Murine models of ADPKD represent invaluable tools for studying this disease. These murine forms of ADPKD can arise spontaneously, or they can be induced via chemical or genetic manipulations. Although these models have improved our understanding of the etiology and pathogenesis of ADPKD, they have not led to effective treatment strategies. The mini-pig represents an effective biomedical model for studying human diseases, as the pig's human-like physiological processes help to understand disease mechanisms and to develop novel therapies. Here, we tried to generate a transgenic model of ADPKD in pigs by overexpressing c-Myc in kidney tissue. Western-blot analysis showed that c-Myc was overexpressed in the kidney, brain, heart, and liver of transgenic pigs. Immunohistochemical staining of kidney tissue showed that exogenous c-Myc predominantly localized to renal tubules. Slightly elevated blood urea nitrogen levels were observed in transgenic pigs 1 month after birth, but no obvious abnormalities were detected after that time. In the future, we plan to subject this model to renal injury in an effort to promote ADPKD progression.

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Isolation and characterization of the porcine c-myc proto-oncogene and chromosomal assignment to SSC 4p13

Cited by 7 publications

References 14 publications

The analysis of carcass value in pigs of different genotypes

The analysis of carcass value in pigs of different genotypes

Polyvinylamine hydrochloride-based microcapsules: polymer synthesis, permeability and mechanical properties

Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease

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