Isolation and characterization of Thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum “Chloroflexi”, isolated from a deep hot aquifer in the Aquitaine Basin

Grégoire, Philippe; Fardeau, Marie Laure; Joseph, Manon; Guasco, Sophie; Hamaide, Francette; Biasutti, Sandra; Michotey, Valérie; Bonin, Patricia; Ollivier, Bernard

doi:10.1016/j.syapm.2011.02.004

Cited by 72 publications

(30 citation statements)

References 20 publications

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“…Species (bands 7, 19, and 20) belonging to class Anaerolineae (phyla Chloroflexi) were mainly located in the upper layer, which was similar to the distribution pattern of microbial diversity observed at the phylum level in a full-scale UASB reactor for treatment of domestic wastewater (de Lucena et al 2011). This was likely due to the strictly anaerobic environment in the upper layer of the EGSB (Gregoire et al 2011) with the lower ORP value (−503 mV) (Table 1). Furthermore, species (bands 13 and 15) belonging to Firmicutes were associated with biogas production of hydrogen and carbon dioxide in the EGSB reactor, ending in reduction of acetate (Table 2).…”

Section: Phylogenetic Diversity Of Microorganisms In Egsb Reactorsupporting

confidence: 75%

Microbial community analysis in a combined anaerobic and aerobic digestion system for treatment of cellulosic ethanol production wastewater

Shan

Zhu

et al. 2015

Environ Sci Pollut Res

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This study investigated the microbial diversity established in a combined system composed of a continuous stirred tank reactor (CSTR), expanded granular sludge bed (EGSB) reactor, and sequencing batch reactor (SBR) for treatment of cellulosic ethanol production wastewater. Excellent wastewater treatment performance was obtained in the combined system, which showed a high chemical oxygen demand removal efficiency of 95.8% and completely eliminated most complex organics revealed by gas chromatography-mass spectrometry (GC-MS). Denaturing gradient gel electrophoresis (DGGE) analysis revealed differences in the microbial community structures of the three reactors. Further identification of the microbial populations suggested that the presence of Lactobacillus and Prevotella in CSTR played an active role in the production of volatile fatty acids (VFAs). The most diverse microorganisms with analogous distribution patterns of different layers were observed in the EGSB reactor, and bacteria affiliated with Firmicutes, Synergistetes, and Thermotogae were associated with production of acetate and carbon dioxide/hydrogen, while all acetoclastic methanogens identified belonged to Methanosaetaceae. Overall, microorganisms associated with the ability to degrade cellulose, hemicellulose, and other biomass-derived organic carbons were observed in the combined system. The results presented herein will facilitate the development of an improved cellulosic ethanol production wastewater treatment system.

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Section: Phylogenetic Diversity Of Microorganisms In Egsb Reactorsupporting

confidence: 75%

Microbial community analysis in a combined anaerobic and aerobic digestion system for treatment of cellulosic ethanol production wastewater

Shan

Zhu

et al. 2015

Environ Sci Pollut Res

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“…This indicates that Fervidobacterium works as a major hydrogen producer in this community. Additionally, F. pennavorans (clone NKB_H66_39) (3), T. daxensis (clone NKB_H66_24) (5), D. turgidum (clone NKB_H66_40) (42) and I. album (clone NKB_H66_03) (17) are possible candidates as hydrogen producers by means of their fermentative metabolism.…”

Section: Discussionmentioning

confidence: 99%

Production and Consumption of Hydrogen in Hot Spring Microbial Mats Dominated by a Filamentous Anoxygenic Photosynthetic Bacterium

et al. 2012

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Microbial mats containing the filamentous anoxygenic photosynthetic bacterium Chloroflexus aggregans develop at Nakabusa hot spring in Japan. Under anaerobic conditions in these mats, interspecies interaction between sulfate-reducing bacteria as sulfide producers and C. aggregans as a sulfide consumer has been proposed to constitute a sulfur cycle; however, the electron donor utilized for microbial sulfide production at Nakabusa remains to be identified. In order to determine this electron donor and its source, ex situ experimental incubation of mats was explored. In the presence of molybdate, which inhibits biological sulfate reduction, hydrogen gas was released from mat samples, indicating that this hydrogen is normally consumed as an electron donor by sulfate-reducing bacteria. Hydrogen production decreased under illumination, indicating that C. aggregans also functions as a hydrogen consumer. Small amounts of hydrogen may have also been consumed for sulfur reduction. Clone library analysis of 16S rRNA genes amplified from the mats indicated the existence of several species of hydrogen-producing fermentative bacteria. Among them, the most dominant fermenter, Fervidobacterium sp., was successfully isolated. This isolate produced hydrogen through the fermentation of organic carbon. Dispersion of microbial cells in the mats resulted in hydrogen production without the addition of molybdate, suggesting that simultaneous production and consumption of hydrogen in the mats requires dense packing of cells. We propose a cyclic electron flow within the microbial mats, i.e. , electron flow occurs through three elements: S (elemental sulfur, sulfide, sulfate), C (carbon dioxide, organic carbon) and H (di-hydrogen, protons).

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“…In this decade, 9 species, Anaerolinea thermophila , Anaerolinea thermolimosa , Levilinea saccharolytica , Leptolinea tardivitalis , Bellilinea caldifistulae , Longilinea arvoryzae , Thermanaerothrix daxensis and Ornatilinea apprima , have been isolated (6, 21, 32). The members of A. thermophila , A. thermolimosa , L. saccharolytica , L. tardivitalis and B. caldifistulae were isolated from methanogenic sludges under mesophilic or thermophilic conditions.…”

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confidence: 99%

“…The members of A. thermophila , A. thermolimosa , L. saccharolytica , L. tardivitalis and B. caldifistulae were isolated from methanogenic sludges under mesophilic or thermophilic conditions. In contrast to these species, L. arvoryzae was obtained from a rice field soil, and two species of T. daxensis and O. apprima were derived from terrestrial subsurface hot aquifers (6, 21, 31). Furthermore, isolation of two marine strains from an enrichment reactor of subseafloor sediments was reported recently (10).…”

mentioning

confidence: 99%

“…These species have similar physiological characteristics, such as being obligately anaerobic, mesophilic to moderately thermophilic, and multicellular filamentous heterotrophs utilizing carbohydrates and amino acids (or peptides). All the Anaerolineae species except for O. apprima are slowly growing microorganisms with generation times of 10–100 hours under the optimum growth conditions (6, 21, 32). …”

mentioning

confidence: 99%

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Isolation and Characterization of a Thermophilic, Obligately Anaerobic and Heterotrophic Marine Chloroflexi Bacterium from a Chloroflexi-dominated Microbial Community Associated with a Japanese Shallow Hydrothermal System, and Proposal for Thermomarinilinea lacunofontalis gen. nov., sp. nov.

et al. 2013

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A novel marine thermophilic and heterotrophic Anaerolineae bacterium in the phylum Chloroflexi, strain SW7T, was isolated from an in situ colonization system deployed in the main hydrothermal vent of the Taketomi submarine hot spring field located on the southern part of Yaeyama Archipelago, Japan. The microbial community associated with the hydrothermal vent was predominated by thermophilic heterotrophs such as Thermococcaceae and Anaerolineae, and the next dominant population was thermophilic sulfur oxidizers. Both aerobic and anaerobic hydrogenotrophs including methanogens were detected as minor populations. During the culture-dependent viable count analysis in this study, an Anaerolineae strain SW7T was isolated from an enrichment culture at a high dilution rate. Strain SW7T was an obligately anaerobic heterotroph that grew with fermentation and had non-motile thin rods 3.5–16.5 μm in length and 0.2 μm in width constituting multicellular filaments. Growth was observed between 37–65°C (optimum 60°C), pH 5.5–7.3 (optimum pH 6.0), and 0.5–3.5% (w/v) NaCl concentration (optimum 1.0%). Based on the physiological and phylogenetic features of a new isolate, we propose a new species representing a novel genus Thermomarinilinea: the type strain of Thermomarinilinea lacunofontalis sp. nov., is SW7T (=JCM15506T=KCTC5908T).

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Isolation and characterization of Thermanaerothrix daxensis gen. nov., sp. nov., a thermophilic anaerobic bacterium pertaining to the phylum “Chloroflexi”, isolated from a deep hot aquifer in the Aquitaine Basin

Cited by 72 publications

References 20 publications

Microbial community analysis in a combined anaerobic and aerobic digestion system for treatment of cellulosic ethanol production wastewater

Microbial community analysis in a combined anaerobic and aerobic digestion system for treatment of cellulosic ethanol production wastewater

Production and Consumption of Hydrogen in Hot Spring Microbial Mats Dominated by a Filamentous Anoxygenic Photosynthetic Bacterium

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