Isolation and characterization of thermophilic cellulose and hemicellulose degrading bacterium, Thermoanaerobacterium sp. R63 from tropical dry deciduous forest soil

Abstract

Thermophilic microorganisms and their enzymes have been utilized in various industrial applications. In this work, we isolated and characterized thermophilic anaerobic bacteria with the cellulose and hemicellulose degrading activities from a tropical dry deciduous forest in northern Thailand. Out of 502 isolated thermophilic anaerobic soil bacteria, 6 isolates, identified as Thermoanaerobacterium sp., displayed an ability to utilize a wide range of oligosaccharides and lignocellulosic substrates. The isolates exhibited significant cellulase and xylanase activities at high temperature (65˚C). Among all isolates, Thermoanaerobacterium sp. strain R63 exhibited remarkable hydrolytic properties with the highest cellulase and xylanase activities at 1.15 U/mg and 6.17 U/mg, respectively. Extracellular extract of Thermoanaerobacterium sp. strain R63 was thermostable with an optimal temperature at 65˚C and could exhibit enzymatic activities on pH range 5.0-9.0. Our findings suggest promising applications of these thermoanaerobic bacteria and their potent enzymes for industrial purposes.

Screening, cloning, enzymatic properties of a novel thermostable cellulase enzyme, and its potential application on water hyacinth utilization

“…The optimal temperature of Cel1029 is slightly higher than that of Lp-egl-1 from Lyrodus pedicellatus (Hiroshi et al 2008) and PersiCel1 from camel rumen metagenome ). On the basis of hot activity, Cel1029 outperforms PersiCel2 with about 10% activity at 80°C , but is worse than R63 from Thermoanaerobacterium sp with about 80% activity at 75°C, (Harnvoravongchai et al 2020) and PW2 from Bacillus sp. with 100% activity at 80°C (Divyanshi et al 2019).…”

Isolation and characterization of potential multiple extracellular enzyme-producing bacteria from waste dumping area in Addis Ababa

“…Therefore, the observed isolates are thermophilic bacteria ( Simandjuntak and Mokosuli, 2018 ). Furthermore, the soil waste sample from which thermophilic bacteria was isolated was high and consistedof organic materials; thus, bacteria isolated from such places may have better potential to produce critical industrial compounds under adverse conditions ( Harnvoravongchai et al, 2020 ).…”

“…Determining the hydrolysis capacity (H.C.) value is more feasible for screening the degrading bacteria in large sample volumes than an enzymatic assay ( Harnvoravongchai et al, 2020 ). The clear zones on the different mediums were used to conduct qualitative testing of extracellular enzyme-producing bacteria.…”

Biomass Valorization: Sustainable Methods for the Production of Hemicellulolytic Catalysts from Thermoanaerobacterium thermostercoris strain BUFF

“…Furthermore, the crude enzymes of strain BUFF exhibited up to 100-fold greater xylanase activity than that reported in T. xylanolyticum strain B6A-RI (0.065 U/mg) and T. brockii strain HTD4 (0.003 U/ mg) [38]. In the genus Thermoanaerobacterium, strains equipped with multi-enzymatic systems capable of digesting lignocellulose are also described; a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1 when grown on xylan showed a multienzyme complex consisting of xylanase, β-xylosidase, α-L-arabinofuranosidase, β-glucosidase and cellobiohydrolase [40,41].…”