Isolation and characterization of thirteen polymorphic microsatellite loci in the A‐genome perennial group of the legume genus <i>Glycine</i>

Bronski, Michael J.; Straub, Shannon C. K.; Bogdanowicz, Steven M.; Doyle, Jane L.; Brown, A. H. D.; Doyle, Jeff J.

doi:10.1111/j.1755-0998.2009.02712.x

Cited by 2 publications

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References 12 publications

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“…Results from G. clandestina for 19 loci on eight individuals showed polymorphism that correlated with two morphotypes associated with different habitats highlighted by a previous study (Pfeil et al, 2001). Smaller numbers of SSR loci have been developed for A‐ and B‐genome perennial species (Bronski et al, 2009); although they appear useful in identifying taxa within these groups (S. Gunner and J. J. Doyle, unpublished data; N. Gengel and J. J. Doyle, unpublished data), next‐generation sequencing approaches hold more promise for producing markers for genome‐wide comparisons (Bombarely et al, 2014).…”

Section: Comparisons Between the Annual And Perennial Glycine Speciesmentioning

confidence: 99%

The wild side of a major crop: Soybean's perennial cousins from Down Under

Sherman-Broyles

Bombarely

Powell

et al. 2014

American J of Botany

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The accumulation of over 30 years of basic research on the biology, genetic variation, and evolution of the wild perennial relatives of soybean (Glycine max) provides a foundation to improve cultivated soybean. The cultivated soybean and its wild progenitor, G. soja, have a center of origin in eastern Asia and are the only two species in the annual subgenus Soja. Systematic and evolutionary studies of the ca. 30 perennial species of subgenus Glycine, native to Australia, have benefited from the availability of the G. max genomic sequence. The perennial species harbor many traits of interest to soybean breeders, among them resistance to major soybean pathogens such as cyst nematode and leaf rust. New species in the Australian subgenus continue to be described, due to the collection of new material and to insights gleaned through systematic studies of accessions in germplasm collections. Ongoing studies in perennial species focus on genomic regions that contain genes for key traits relevant to soybean breeding. These comparisons also include the homoeologous regions that are the result of polyploidy in the common ancestor of all Glycine species. Subgenus Glycine includes a complex of recently formed allopolyploids that are the focus of studies aimed at elucidating genomic, transcriptomic, physiological, taxonomic, morphological, developmental, and ecological processes related to polyploid evolution. Here we review what has been learned over the past 30 years and outline ongoing work on photosynthesis, nitrogen fixation, and floral biology, much of it drawing on new technologies and resources.

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Section: Comparisons Between the Annual And Perennial Glycine Speciesmentioning

confidence: 99%

The wild side of a major crop: Soybean's perennial cousins from Down Under

Sherman-Broyles

Bombarely

Powell

et al. 2014

American J of Botany

Self Cite

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Identification of high-quality single-nucleotide polymorphisms in Glycine latifolia using a heterologous reference genome sequence

et al. 2013

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Like many widely cultivated crops, soybean [Glycine max (L.) Merr.] has a relatively narrow genetic base, while its perennial distant relatives in the subgenus Glycine Willd. are more genetically diverse and display desirable traits not present in cultivated soybean. To identify single-nucleotide polymorphisms (SNPs) between a pair of G. latifolia accessions that were resistant or susceptible to Sclerotinia sclerotiorum (Lib.) de Bary, reduced-representations of DNAs from each accession were sequenced. Approximately 30 % of the 36 million 100-nt reads produced from each of the two G. latifolia accessions aligned primarily to gene-rich euchromatic regions on the distal arms of G. max chromosomes. Because a genome sequence was not available for G. latifolia, the G. max genome sequence was used as a reference to identify 9,303 G. latifolia SNPs that aligned to unique positions in the G. max genome with at least 98 % identity and no insertions and deletions. To validate a subset of the SNPs, nine TaqMan and 384 GoldenGate allele-specific G. latifolia SNP assays were designed and analyzed in F2 G. latifolia populations derived from G. latifolia plant introductions (PI) 559298 and 559300. All nine TaqMan markers and 91 % of the 291 polymorphic GoldenGate markers segregated in a 1:2:1 ratio. Genetic linkage maps were assembled for G. latifolia, nine of which were uninterrupted and nearly collinear with the homoeologous G. max chromosomes. These results made use of a heterologous reference genome sequence to identify more than 9,000 informative high-quality SNPs for G. latifolia, a subset of which was used to generate the first genetic maps for any perennial Glycine species.

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Isolation and characterization of thirteen polymorphic microsatellite loci in the A‐genome perennial group of the legume genus Glycine

Cited by 2 publications

References 12 publications

The wild side of a major crop: Soybean's perennial cousins from Down Under

The wild side of a major crop: Soybean's perennial cousins from Down Under

Identification of high-quality single-nucleotide polymorphisms in Glycine latifolia using a heterologous reference genome sequence

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