Isolation and characterization of thirteen microsatellite markers for the rabbitfish, Siganus fuscescens

Ravago-Gotanco, Rachel; Lumibao, Candice Y.; Pante, Ma. Josefa R.

doi:10.1007/s12686-009-9148-y

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…Genomic DNA was extracted using DNeasy Blood and Tissue kits (QIAGEN, Valencia, CA, USA). Samples were genotyped at five microsatellite loci originally designed for the S. spinus congener S. fuscescens (Ravago‐Gotanco et al 2010). The loci used in this study were Sfus‐8, Sfus‐9, Sfus‐56, Sfus‐98, and Sfus‐113 .…”

Section: Methodsmentioning

confidence: 99%

Evidence of stable genetic structure across a remote island archipelago through self‐recruitment in a widely dispersed coral reef fish

Priest¹,

Halford²,

McIlwain³

2012

Ecology and Evolution

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We used microsatellite markers to assess the population genetic structure of the scribbled rabbitfish Siganus spinus in the western Pacific. This species is a culturally important food fish in the Mariana Archipelago and subject to high fishing pressure. Our primary hypothesis was to test whether the individuals resident in the southern Mariana Island chain were genetically distinct and hence should be managed as discrete stocks. In addition to spatial sampling of adults, newly-settled individuals were sampled on Guam over four recruitment events to assess the temporal stability of the observed spatial patterns, and evidence of self-recruitment. We found significant genetic structure in S. spinus across the western Pacific, with Bayesian analyses revealing three genetically distinct clusters: the southern Mariana Islands, east Micronesia, and the west Pacific; with the southern Mariana Islands being more strongly differentiated from the rest of the region. Analyses of temporal samples from Guam indicated the southern Mariana cluster was stable over time, with no genetic differentiation between adults versus recruits, or between samples collected across four separate recruitment events spanning 11 months. Subsequent assignment tests indicated seven recruits had self-recruited from within the Southern Mariana Islands population. Our results confirm the relative isolation of the southern Mariana Islands population and highlight how local processes can act to isolate populations that, by virtue of their broad-scale distribution, have been subject to traditionally high gene flows. Our results add to a growing consensus that self-recruitment is a highly significant influence on the population dynamics of tropical reef fish.

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Section: Methodsmentioning

confidence: 99%

Evidence of stable genetic structure across a remote island archipelago through self‐recruitment in a widely dispersed coral reef fish

Priest¹,

Halford²,

McIlwain³

2012

Ecology and Evolution

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“…Individuals were genotyped at 12 microsatellite markers previously developed for S . fuscescens : Sfus6 , Sfus8 , Sfus9 , Sfus21 , Sfus22 , Sfus56 , Sfus76 , Sfus95 , Sfus97 , Sfus98 , Sfus113 , and Sfus167 [ 24 ]. Microsatellite DNA fragments were PCR-amplified in four separate multiplex reactions.…”

Section: Methodsmentioning

confidence: 99%

“…Amplification conditions consisted of 94°C for 90 s, 38 cycles of 94°C for 30 s, locus-specific annealing temperatures for 30 s, 72°C for 60 s and a final extension at 72°C for 25 minutes. Annealing temperatures, fluorescent label-primer combinations, and pooling of amplified microsatellite loci for fragment analysis have been described elsewhere [ 24 ]. Amplified fragments were combined with an internal size standard, GeneScan TM 500 LIZ TM , Hi-Di TM formamide (Applied Biosystems Inc., Carlsbad CA) and deionized water to a final volume of 10 μL.…”

Section: Methodsmentioning

confidence: 99%

Cryptic genetic diversity in the mottled rabbitfish Siganus fuscescens with mitochondrial introgression at a contact zone in the South China Sea

et al. 2018

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The taxonomy of the mottled rabbitfish Siganus fuscescens species complex has long been challenging. In this study, we analyzed microsatellite genotypes, mitochondrial lineages, and morphometric data from 373 S. fuscescens individuals sampled from the northern Philippines and Hong Kong (South China Sea, Philippine Sea and Sulu Sea basins), to examine putative species boundaries in samples comprising three co-occurring mitochondrial lineages previously reported to characterize S. fuscescens (Clade A and Clade B) or S. canaliculatus (Clade C). We report the existence of two cryptic species within S. fuscescens in the northeast region of the South China Sea and northern Philippine Sea, supported by genetic and morphological differences. Individual-based assignment methods recovered concordant groupings of individuals into two nuclear genotype clusters (Cluster 1, Cluster 2) with (1) limited gene flow, if any, between them (FST = 0.241; P < 0.001); (2) low frequency of later-generation hybrids; (3) significant association with mitochondrial Clade A and Clade B, respectively; and (4) subtle yet significant body shape differences as inferred from geometric morphometric analysis. The divergence between mitochondrial Clade C and the two other clades was not matched by genetic differences at microsatellite marker loci. The occurrence of discordant mitonuclear combinations (20.5% of the total number of individuals) is thought to result from mitochondrial introgression, consistent with a scenario of demographic, and presumably spatial, post-Pleistocene expansion of populations from northern regions into a secondary contact zone in the South China Sea. Mitonuclear discordance due to introgression obscures phylogenetic relationships for recently-diverged lineages, and cautions against the use of mitochondrial markers alone for species identification within the mottled rabbitfish species complex in the South China Sea region.

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Genetic divergence and phylogenetic relationship of the rabbitfish Siganus rivulatus inferred from microsatellite and mitochondrial markers

Hashem

Abdelsalam

Gaber

et al. 2022

Journal of King Saud University - Science

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Isolation and characterization of thirteen microsatellite markers for the rabbitfish, Siganus fuscescens

Cited by 3 publications

References 8 publications

Evidence of stable genetic structure across a remote island archipelago through self‐recruitment in a widely dispersed coral reef fish

Evidence of stable genetic structure across a remote island archipelago through self‐recruitment in a widely dispersed coral reef fish

Cryptic genetic diversity in the mottled rabbitfish Siganus fuscescens with mitochondrial introgression at a contact zone in the South China Sea

Genetic divergence and phylogenetic relationship of the rabbitfish Siganus rivulatus inferred from microsatellite and mitochondrial markers

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