Isolation and characterization of Toxoplasma gondii strains from stray cats revealed a single genotype in Beijing, China

Qian, Weifeng; Wang, Hui; Su, Chunlei; Shan, Dan; Cui, Xiuhong; Yang, Na; Lv, Chaochao; Liu, Qun

doi:10.1016/j.vetpar.2012.01.026

Cited by 51 publications

(35 citation statements)

References 24 publications

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“…In this investigation, the overall seroprevalence of T. gondii in the examined cats was 19.34 % (70/362), which was lower than that in cats in other places of China (Dubey et al 2007;Qian et al 2012) but similar to a previous study reporting an overall 21.3 % seroprevalence of T. gondii among cats in Lanzhou (Wu et al 2011). However, the various methodologies used and greatly different sample sizes and sample populations in the countries surveyed may contribute to these difference so that it is difficult to compare reported seroprevalence.…”

Section: Discussionsupporting

confidence: 84%

“…In this study, ToxoDB#9 was identified to be prevalent in this region. In previous studies, this same genotype was frequently identified in cats in many regions in China (Dubey et al 2007;Chen et al 2011;Qian et al 2012;Wang et al 2013;Tian et al 2014), and it was also found in humans, pigs, goats, and other species Wang et al 2012;Cong et al 2015;Miao et al 2015). Hence, ToxoDB#9 is considered to be a preponderant lineage prevalent in China.…”

Section: Discussionmentioning

confidence: 70%

“…Epidemiological data are necessary to define preventive, management, and therapeutical measures for stray, feral, and domestic cats. Although several studies have reported the T. gondii prevalence in cats in various regions in China (Dubey et al 2007;Chen et al 2011;Qian et al 2012;Wang et al 2013;Tian et al 2014), including Lanzhou (Wu et al 2011), little is known of the genotypes of T. gondii in cats in northwest China. Here, we investigated the genotypes of T. gondii in cats in northwest China for the first time.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available. The overall prevalence of T. gondii, FIV, FeLV, and D. immitis was 19.34, 9.12, 11.33, and 3.04 %, respectively. For the genetic characterization of T. gondii genotypes in cats, genomic DNA was extracted from the seropositive cats and the T. gondii B1 gene was amplified using a semi-nested PCR. DNA samples giving positive B1 amplification were then genotyped using multilocus PCR-RFLP. Two T. gondii genotypes (ToxoDB#9 and ToxoDB#1) were identified. Results of the multivariate logistic regression analysis showed that older cats are more likely to be seropositive than juveniles for T. gondii, FIV, FeLV, and D. immitis. This is the first report of T. gondii genotypes in cats in northwest China. Moreover, the present study is the first study of retrovirus and D. immitis seroprevalence in cats in China. The results revealed that T. gondii, FIV, and FeLV infections are common in stray and pet cats in northwest China.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

et al. 2015

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“…However, the KO mice are very costly. To overcome this, Qian et al (2012) immunosuppressed outbred mice by dexamethasone medication.…”

Section: Discussionmentioning

confidence: 99%

Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China

Yang

Ying

Verma

et al. 2015

Veterinary Parasitology

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“…gondii is distributed worldwide and factors such as geographical, environmental and cultural habits have been known to influence the prevalence of the parasite especially in tropical and subtropical regions of the world (Dubey, 2010). Serological studies carried out worldwide indicate varying prevalence rates of T. gondii infection with 7% in Nigeria (Alayande et al, 2012), 57.8% in China (Qian et al, 2012), 63% in Albania (Silaghi et al, 2014) and 10% -87% in Brazil Cerro et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Serologic survey of Toxoplasma gondii antibodies in cats (Felis catus) sold at live animal markets in Southwestern Nigeria

Ayinmode¹,

Oluwayelu²,

Babalola³

et al. 2017

BJVM

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Toxoplasma gondii is a zoonotic parasite causing infection in humans and a wide range of mammals, with cats being the final and only host that excrete T. gondii-resistant oocysts to the environment. This study was designed to investigate the seroprevalence of antibodies to T. gondii in cats sold at Live Animal Markets (LAMs) in Ibadan, Osogbo and Offa, Southwestern Nigeria. Blood samples were randomly collected from 226 cats at these markets and separated sera were tested for the presence of antibodies (IgG) to T. gondii, using two-fold dilutions from 1:20 to 1:320 by the Modified Agglutination Test (MAT). Attributes were analysed using Chi-square and Fisher's exact tests at P<0.05. Median age of cats was 4.0 months (range: 1-36 months). Of the 226 samples tested, only 10 (4.4%) were positive for anti-T. gondii IgG antibodies at the cut-off titre of 1:20. Nine (4.0%) gave a titre of 1:20; one (0.4%) gave 1:80 while none was positive at 1:40, 1:160 and 1:320 dilutions. Age and gender of cats was not significantly associated with T. gondii infection. Our results showed low prevalence of T. gondii infection in cats sold at LAMs in studied area and suggests confinement as an efficient way of limiting exposure of cats to infection sources.

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Isolation and characterization of Toxoplasma gondii strains from stray cats revealed a single genotype in Beijing, China

Cited by 51 publications

References 24 publications

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections in stray and pet cats (Felis catus) in northwest China: co-infections and risk factors

Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China

Serologic survey of Toxoplasma gondii antibodies in cats (Felis catus) sold at live animal markets in Southwestern Nigeria

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