Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos

Suasnavas, Edison; Heywood, Sierra; Ward, Anika; Cox, Lindsay; O'Grady, Merecedes; Zhao, Yuanfeng; Gilbert, Lacey; Isom, S. Clay

doi:10.1016/j.anireprosci.2015.01.012

Cited by 10 publications

(6 citation statements)

References 35 publications

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“…Thus, a narrow range of SOX2 expression is needed to prevent differentiation (Kopp et al, ). Indeed, cessation of SOX2 expression is observed in the in vivo‐derived trophectdoderm of pre‐implantation porcine blastocysts compared to the epiblast and hypoblast tissue (du Puy et al, ; Fujii et al, ; Gao et al, ), while porcine trophectoderm stem cell lines displayed no SOX2 expression (Suasnavas et al, ). Similarly, SOX2 expression is down‐regulated in the trophectoderm of elongating bovine blastocysts (Degrelle et al, ).…”

Section: Discussionmentioning

confidence: 99%

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

et al. 2017

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Thirteen independent induced bovine trophectroderm (iBT) cell lines were established by reprogramming bovine fetal liver-derived fibroblasts after viral-vector transduction with either six or eight factors, including POU5F1 (OCT4), KLF4, SOX2, MYC, NANOG, LIN28, SV40 large T antigen, and hTERT. Light- and electron-microscopy analysis showed that the iBT cells had epithelial cell morphology typical of bovine trophectoderm cells. Reverse-transcription-PCR assays indicated that all of the cell lines expressed interferon-tau (IFNT) at passages 1 or 2. At later passages (≥ passage 8), however, immunoblot and antiviral activity assays revealed that more than half of the iBT cell lines had stopped expressing IFNT. Messenger RNAs specific to trophectoderm differentiation and function were found in the iBT cell lines, and 2-dimensional-gel analysis for cellular proteins showed an expression pattern similar to that of trophectoderm cell lines derived from bovine blastocysts. Integration of some of the human reprogramming factors, including POU5F1, KLF4, SOX2, MYC, NANOG, and LIN28, were detected by PCR, but their transcription was mostly absent in the iBT cell lines. Gene expression assessment of endogenous bovine reprogramming factor orthologs revealed endogenous bLIN28 and bMYC transcripts in all; bSOX2 and bNANOG in none; and bKLF4 and bPOU5F1 in less than half of the iBT cell lines. These results demonstrate that bovine trophectoderm can be induced via reprogramming factor expression from bovine liver-derived fibroblasts, although other fibroblast populations-e.g., derived from fetal thigh tissue-may produce similar results, albeit at lower frequencies.

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Section: Discussionmentioning

confidence: 99%

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

et al. 2017

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“…As previously described by McGill et al (2016) , RNA was isolated from muscle samples using Trizol Reagent (Life Technologies, Carlsbad, CA, USA), and, after RNA isolation, cDNA was created using Superscript III Reverse Transcriptase and random primers (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions. Gene expression analysis was performed using a 96.96 Dynamic Array Integrated Fluidic Circuit (Fluidigm, San Francisco, CA, USA) as previously described by Suasnavas et al (2015) . Supplemental Table S1 lists the 24 targeted genes used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Heiderscheit

Hansen

2022

Journal of Animal Science

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Fifty-four Angus-cross steers (297 kg ± 12) were stratified by body weight (BW) to pens (6 steers per pen) to determine effects of supplemental Zn on post-transit growth performance and blood and muscle metabolites. Dietary treatments started 25 d before trucking: control (CON; analyzed 54 mg Zn/kg DM), industry (IND; CON + 70 mg supplemental Zn/kg DM), and supranutritional Zn (SUPZN; CON + 120 mg supplemental Zn/kg DM). Supplemental Zn was bis-glycinate bound Zn (Plexomin Zn; Phytobiotics North America, Cary, NC). On d 0, steers were loaded onto a commercial trailer and transported 18 h (1,822 km). Individual BW were recorded on d -26, -25, -1, 0 (pre-transit), 1 (post-transit), 6, 27, and 28. Blood was collected on d -1, 1, 6, and 27. Longissimus thoracis biopsies were collected on d -1, 1, and 28. Daily individual feed disappearance was recorded via GrowSafe bunks. Data were analyzed using Proc Mixed of SAS with fixed effect of diet and steer as the experimental unit (growth performance, blood: n = 18 steers per treatment; muscle: n = 12 steers per treatment). Individual initial BW was used as a covariate in BW analysis. Contrast statements to test linear, quadratic, and Zn effects were used to analyze performance and blood parameters. Repeated measures analysis was used for post-transit DMI recovery and weekly post-transit DMI and Zn intake with the repeated effect of time. MetaboAnalyst 5.0 was utilized for statistical analysis of d 1 (off truck) muscle metabolites. Plasma Zn linearly increased due to Zn on d 1, 6, and 27 (P = 0.01), and off-truck (d 1) serum lactate increased over d -1 by 20, 0, and 20% in CON, IND, and SUPZN, respectively (Quadratic: P = 0.01). Muscle lactate tended to increase post-transit in CON and IND (P ≤ 0.07) but not SUPZN. Muscle metabolites relating to amino acid and nitrogen metabolism were increased in all treatments post-transit (P ≤ 0.02), and alanine-glucose cycle metabolites tended to increase in CON and IND (P ≤ 0.07). Steers supplemented with Zn recovered pre-transit DMI quicker than CON (by d 2: P = 0.01), while IND had greater overall post-transit DMI than CON with SUPZN intermediate (P = 0.04), and Zn-fed steers had greater ADG post-transit (P = 0.04). Zinc supplementation mitigated muscle or serum lactate increases due to transit and increased post-transit ADG.

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“…). Primer sets (see S1 Table for primer sequences) had been comprehensively validated to have similar amplification efficiencies previously [25].…”

Section: Cyp17a1 Elf5 Hand1 Hsd17b1 Krt8 Tead4mentioning

confidence: 99%

Effects of electrical biostimulation and silver ions on porcine fibroblast cells

2021

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The medical applications of electrical biostimulation and silver ions have been evaluated in laboratory experiments and clinical studies for more than two decades. Their effects on preventing infection and promoting wound healing have been described. However, little is known about the role of electrical biostimulation and/or silver ion on changes in cellular transcriptome dynamics. To our knowledge, few studies have been conducted to investigate the potential of electrical biostimulation and silver ions in cell reprogramming. Besides, it is essential to assess any possible adverse effects or potential benefits of the silver ions on mammalian cells to address its safety concerns and to improve silver medical products. In this study, we investigated transcriptomic changes in porcine fibroblast cells in response to electrical biostimulation in the presence of silver ions. Exposed cells presented distinct morphological changes after treatment, which was mainly due to the exposure of silver ions rather than the electrical current itself. Gene expression analyses suggested that electrical biostimulation and silver ions did not increase the expression of pluripotency genes. Interestingly, a set of genes related to cellular metabolic processes were differentially expressed after cells were exposed to electrically generated silver ions for 21 hours. We found that 2.00 mg/L of electrically generated silver ion caused an increase of ATP generation and an increase of the total pool of NAD+ and NADH, while ROS production did not change. Aside from toxic effects, the results reported herein demonstrate the alternative effects of silver ions on mammalian cells, especially an oxidative phosphorylation burst. To our knowledge, this response of mammalian cells to silver ions has not been described previously. Although the function of this burst is not understood, it may lead to alterations in cellular activities such as metabolic remodeling and cell reprogramming, and/or serve an as-yet unknown function in neutralization or detoxification of the silver ions within the cells.

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Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos

Cited by 10 publications

References 35 publications

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Effects of electrical biostimulation and silver ions on porcine fibroblast cells

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