Isolation and Characterization of Two Viruses with Large Genome Size Infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae)

Sandaa, Ruth‐Anne; Heldal, Mikal; Castberg, Tonje; Thyrhaug, Runar; Bratbak, Gunnar

doi:10.1006/viro.2001.1161

Cited by 137 publications

(131 citation statements)

References 47 publications

Supporting

Mentioning

127

Contrasting

Order By: Relevance

“…However, we found that prasinovirus-like phylotypes (and KBvp-8 in particular) dominated both the winter 2006 sample, which was prefiltered, and the summer samples, which were not. Some PCR bias does seem likely, as it has been reported that the AVS primers do not amplify the DNA polymerase gene of several representative members of genera within the family (Sandaa et al, 2001;Nagasaki et al, 2005). Different approaches, such as quantitative PCR assays targeting specific viral groups, will be needed to determine the actual in situ abundance of prasino-like viruses relative to other genera within the family Phycodnaviridae.…”

Section: Discussionmentioning

confidence: 99%

Detection of inteins among diverse DNA polymerase genes of uncultivated members of the Phycodnaviridae

2008

View full text Add to dashboard Cite

Viruses in the family Phycodnaviridae infect autotrophic protists in aquatic environments. Application of a PCR assay targeting the DNA polymerase of viruses in this family has revealed that phycodnaviruses are quite diverse and appear to be widespread, but a limited number of environments have been examined so far. In this study, we examined the sequence diversity among viral DNA pol genes amplified by PCR from subtropical coastal waters of O'ahu, Hawai'i. A total of 18 novel prasinovirus-like sequences were detected along with two other divergent sequences that differ at the genus-level relative to other sequences in the family. Of the 20 new sequence types reported here, three were serendipitously found to contain protein introns, or inteins. Sequence analysis of the inteins suggested that all three have self-splicing domains and are apparently capable of removing themselves from the translated polymerase protein. Two of the three also appear to be 'active', meaning they encode all the motifs necessary for a complete dodecapeptide homing endonuclease, and are therefore capable of horizontal transfer. A subsequent PCR survey of our samples with intein-specific primers suggested that intein-containing phycodnaviruses are common in this environment. A search for similar sequences in metagenomic data sets from other oceans indicated that viral inteins are also widespread, but how these genetic parasites might be influencing the ecology and evolution of phycodnaviruses remains unclear.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of inteins among diverse DNA polymerase genes of uncultivated members of the Phycodnaviridae

2008

View full text Add to dashboard Cite

show abstract

“…To investigate the presence of Mimivirus-like mutS gene in other giruses, we have undertaken a genomic sequencing survey of four giruses previously isolated from marine environments. The four giruses investigated are Pyramimonas orientalis virus (PoV-01B, 560-kb genome), Phaeocystis pouchetii virus (PpV-01B, 485-kb genome), Chrysochromulina ericina virus (CeV-01B, 510-kb genome) and Heterocapsa circularisquama virus (HcDNAV, 356-kb genome) (Jacobsen et al, 1996;Sandaa et al, 2001;Tarutani et al, 2001). The hosts of these viruses are phylogenetically distant and ecologically distinct unicellular marine algae.…”

Section: Introductionmentioning

confidence: 99%

Two new subfamilies of DNA mismatch repair proteins (MutS) specifically abundant in the marine environment

et al. 2011

Self Cite

View full text Add to dashboard Cite

MutS proteins are ubiquitous in cellular organisms and have important roles in DNA mismatch repair or recombination. In the virus world, the amoeba-infecting Mimivirus, as well as the recently sequenced Cafeteria roenbergensis virus are known to encode a MutS related to the homologs found in octocorals and e-proteobacteria. To explore the presence of MutS proteins in other viral genomes, we performed a genomic survey of four giant viruses ('giruses') (Pyramimonas orientalis virus (PoV), Phaeocystis pouchetii virus (PpV), Chrysochromulina ericina virus (CeV) and Heterocapsa circularisquama DNA virus (HcDNAV)) that infect unicellular marine algae. Our analysis revealed the presence of a close homolog of Mimivirus MutS in all the analyzed giruses. These viral homologs possess a specific domain structure, including a C-terminal HNH-endonuclease domain, defining the new MutS7 subfamily. We confirmed the presence of conserved mismatch recognition residues in all members of the MutS7 subfamily, suggesting their role in DNA mismatch repair rather than DNA recombination. PoV and PpV were found to contain an additional type of MutS, which we propose to call MutS8. The MutS8 proteins in PoV and PpV were found to be closely related to homologs from 'Candidatus Amoebophilus asiaticus', an obligate intracellular amoeba-symbiont belonging to the Bacteroidetes. Furthermore, our analysis revealed that MutS7 and MutS8 are abundant in marine microbial metagenomes and that a vast majority of these environmental sequences are likely of girus origin. Giruses thus seem to represent a major source of the underexplored diversity of the MutS family in the microbial world.

show abstract

“…Therefore they play important roles in nutrient (Wilhelm and Suttle 1999) and biogeochemical (Fuhrmann 1999) cycling, and influence structure and diversity of microbial and phytoplankton communities (Fuhrmann 1999, Wommack andColwell 2000). Viruses have also been observed to infect a wide range of aquatic algae (Van Etten et al, 1991, Van Etten andMeints 1999), including bloom-forming marine phytoplankton (Nagasaki et al, 1994a, 1994b, Jacobsen et al, 1996, Nagasaki and Yamaguchi 1997, Sandaa et al, 2001.…”

Section: Introductionmentioning

confidence: 99%

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Kegel

Allen

Metfies

et al. 2007

Gene

View full text Add to dashboard Cite

Blooms of the coccolithophorid Emiliania huxleyi can be infected by viruses, which can lead to bloom-termination. This pilot study used an expressed sequence tag (EST) approach to get a first view of gene-expression changes that occur during viral infection of E. huxleyi. cDNA libraries were constructed from uninfected cultures and 6, 12, and 24 h after infection with E. huxleyi-specific virus 86 (EhV-86). From each library 60-90 ESTs were randomly selected and annotated manually with PhyloGena. Viral genes were identified using BLAST-Search of the known viral genome. The data of this study show, that 6 h after viral infection the algal transcriptome changed significantly although few viral transcripts were present. At this point, changes mainly concerned transcripts related to photosynthesis and protein metabolism. However, after 24 h viral transcripts were most abundant. Viral transcripts found at this stage of viral infection encode proteins involved in protein degradation, nucleic acid degradation, transcription and replication.

show abstract

Isolation and Characterization of Two Viruses with Large Genome Size Infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae)

Cited by 137 publications

References 47 publications

Detection of inteins among diverse DNA polymerase genes of uncultivated members of the Phycodnaviridae

Detection of inteins among diverse DNA polymerase genes of uncultivated members of the Phycodnaviridae

Two new subfamilies of DNA mismatch repair proteins (MutS) specifically abundant in the marine environment

Pilot study of an EST approach of the coccolithophorid Emiliania huxleyi during a virus infection

Contact Info

Product

Resources

About